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A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

Julio E Celis, Peder Madsen, Hanne H Rasmussen, Henrik Leffers, Bent Honoré, Borbala Gesser, Kurt Dejgaard, Eydfinnur Olsen, Nils Magnusson, Jette Kiil, et al. (1991) ELECTROPHORESIS. 12(11). p.802-872
abstract
A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [S-35]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer-aided 2-D gel electrophoresis. The protein numbers in this database differ from those reported in an earlier version due to changes in the scanning hardware (Celis et al., Electrophoresis 1990, 11, 242-254). Annotation categories reported include: "protein name" (listing 207 known proteins in alphabetical order), "basal cell markers", "differentiation markers", "proteins highly up-regulated in psoriatic skin", "microsequenced proteins" and "human autoantigens". For reference, we have also included 2-D gel (isoelectric focusing) patterns of cultured normal and psoriatic keratinocytes, melanocytes, fibroblasts, dermal microvascular endothelial cells, peripheral blood mononuclear cells and sweat duct cells. The keratinocyte 2-D gel protein database will be updated yearly in the November issue of Electrophoresis.
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author
organization
alternative title
A comprehensive 2-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes : towards an integrated approach to the study of cell proliferation, differentiation and skin diseases
year
type
journalArticle (original)
publication status
published
subject
keyword
EXTRACELLULAR-MATRIX, TERMINAL DIFFERENTIATION, POLYACRYLAMIDE GELS, SURFACE RECEPTORS, BASAL CELLS, ELECTROPHORESIS, CULTURE, GROWTH, EXPRESSION, INVITRO
journal title
ELECTROPHORESIS
Electrophoresis
volume
12
issue
11
pages
802 - 872
Web of Science type
Article
ISSN
0173-0835
DOI
10.1002/elps.1150121105
language
English
UGent publication?
yes
classification
A1
id
204159
handle
http://hdl.handle.net/1854/LU-204159
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:38:31
@article{204159,
  abstract     = {A two-dimensional (2-D) gel database of cellular proteins from noncultured, unfractionated normal human epidermal keratinocytes has been established. A total of 2651 [S-35]methionine-labeled cellular proteins (1868 isoelectric focusing, 783 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer-aided 2-D gel electrophoresis. The protein numbers in this database differ from those reported in an earlier version due to changes in the scanning hardware (Celis et al., Electrophoresis 1990, 11, 242-254). Annotation categories reported include: {\textacutedbl}protein name{\textacutedbl} (listing 207 known proteins in alphabetical order), {\textacutedbl}basal cell markers{\textacutedbl}, {\textacutedbl}differentiation markers{\textacutedbl}, {\textacutedbl}proteins highly up-regulated in psoriatic skin{\textacutedbl}, {\textacutedbl}microsequenced proteins{\textacutedbl} and {\textacutedbl}human autoantigens{\textacutedbl}. For reference, we have also included 2-D gel (isoelectric focusing) patterns of cultured normal and psoriatic keratinocytes, melanocytes, fibroblasts, dermal microvascular endothelial cells, peripheral blood mononuclear cells and sweat duct cells. The keratinocyte 2-D gel protein database will be updated yearly in the November issue of Electrophoresis.},
  author       = {Celis, Julio E and Madsen, Peder and Rasmussen, Hanne H and Leffers, Henrik and Honor{\'e}, Bent and Gesser, Borbala and Dejgaard, Kurt and Olsen, Eydfinnur and Magnusson, Nils and Kiil, Jette and Celis, Ariana and Lauridsen, Jette B and Basse, Bodil and Ratz, Gitte P and Andersen, Annette H and Walbum, Else and Brandstrup, Birgitte and Pedersen, Peter S and Brandt, Niels J and Puype, Magda and Van Damme, Jozef and Vandekerckhove, Jo{\"e}l},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {EXTRACELLULAR-MATRIX,TERMINAL DIFFERENTIATION,POLYACRYLAMIDE GELS,SURFACE RECEPTORS,BASAL CELLS,ELECTROPHORESIS,CULTURE,GROWTH,EXPRESSION,INVITRO},
  language     = {eng},
  number       = {11},
  pages        = {802--872},
  title        = {A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases},
  url          = {http://dx.doi.org/10.1002/elps.1150121105},
  volume       = {12},
  year         = {1991},
}

Chicago
Celis, Julio E, Peder Madsen, Hanne H Rasmussen, Henrik Leffers, Bent Honoré, Borbala Gesser, Kurt Dejgaard, et al. 1991. “A Comprehensive Two-dimensional Gel Protein Database of Noncultured Unfractionated Normal Human Epidermal Keratinocytes: Towards an Integrated Approach to the Study of Cell Proliferation, Differentiation and Skin Diseases.” Electrophoresis 12 (11): 802–872.
APA
Celis, J. E., Madsen, P., Rasmussen, H. H., Leffers, H., Honoré, B., Gesser, B., Dejgaard, K., et al. (1991). A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases. ELECTROPHORESIS, 12(11), 802–872.
Vancouver
1.
Celis JE, Madsen P, Rasmussen HH, Leffers H, Honoré B, Gesser B, et al. A comprehensive two-dimensional gel protein database of noncultured unfractionated normal human epidermal keratinocytes: towards an integrated approach to the study of cell proliferation, differentiation and skin diseases. ELECTROPHORESIS. 1991;12(11):802–72.
MLA
Celis, Julio E, Peder Madsen, Hanne H Rasmussen, et al. “A Comprehensive Two-dimensional Gel Protein Database of Noncultured Unfractionated Normal Human Epidermal Keratinocytes: Towards an Integrated Approach to the Study of Cell Proliferation, Differentiation and Skin Diseases.” ELECTROPHORESIS 12.11 (1991): 802–872. Print.