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Proteome analysis of mouse brain exposed to chronic hypoxia

(2011) YAKHTEH. 12(4). p.503-510
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Abstract
Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% 02 for 48 hours. Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca(2+) signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.
Keywords
GeLC-MS/MS, FATTY-ACID SYNTHASE, Proteomics, Mouse, Brain, Chronic Hypoxia, GENE-EXPRESSION, METABOLIC ONCOGENE, CANCER-CELLS, PROTEINS, STRESS, OXYGEN, TOOL, REOXYGENATION, PEPTIDES

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Citation

Please use this url to cite or link to this publication:

Chicago
Gilany, Kambiz, Maarten Aerts, Sylvia Dewilde, Bart Devreese, Luc Moens, and Mohtaram Vafakhah. 2011. “Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia.” Yakhteh 12 (4): 503–510.
APA
Gilany, K., Aerts, M., Dewilde, S., Devreese, B., Moens, L., & Vafakhah, M. (2011). Proteome analysis of mouse brain exposed to chronic hypoxia. YAKHTEH, 12(4), 503–510.
Vancouver
1.
Gilany K, Aerts M, Dewilde S, Devreese B, Moens L, Vafakhah M. Proteome analysis of mouse brain exposed to chronic hypoxia. YAKHTEH. 2011;12(4):503–10.
MLA
Gilany, Kambiz, Maarten Aerts, Sylvia Dewilde, et al. “Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia.” YAKHTEH 12.4 (2011): 503–510. Print.
@article{2040377,
  abstract     = {Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain.
Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7\% 02 for 48 hours.
Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression.
Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca(2+) signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.},
  author       = {Gilany, Kambiz and Aerts, Maarten and Dewilde, Sylvia and Devreese, Bart and Moens, Luc and Vafakhah, Mohtaram},
  issn         = {1561-4921},
  journal      = {YAKHTEH},
  keyword      = {GeLC-MS/MS,FATTY-ACID SYNTHASE,Proteomics,Mouse,Brain,Chronic Hypoxia,GENE-EXPRESSION,METABOLIC ONCOGENE,CANCER-CELLS,PROTEINS,STRESS,OXYGEN,TOOL,REOXYGENATION,PEPTIDES},
  language     = {eng},
  number       = {4},
  pages        = {503--510},
  title        = {Proteome analysis of mouse brain exposed to chronic hypoxia},
  volume       = {12},
  year         = {2011},
}

Web of Science
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