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Proteome analysis of mouse brain exposed to chronic hypoxia

Kambiz Gilany, Maarten Aerts UGent, Sylvia Dewilde, Bart Devreese UGent, Luc Moens and Mohtaram Vafakhah (2011) YAKHTEH. 12(4). p.503-510
abstract
Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% 02 for 48 hours. Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca(2+) signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GeLC-MS/MS, FATTY-ACID SYNTHASE, Proteomics, Mouse, Brain, Chronic Hypoxia, GENE-EXPRESSION, METABOLIC ONCOGENE, CANCER-CELLS, PROTEINS, STRESS, OXYGEN, TOOL, REOXYGENATION, PEPTIDES
journal title
YAKHTEH
Yakhteh
volume
12
issue
4
pages
503 - 510
Web of Science type
Article
Web of Science id
000288889200012
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
0.364 (2011)
JCR rank
99/109 (2011)
JCR quartile
4 (2011)
ISSN
1561-4921
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2040377
handle
http://hdl.handle.net/1854/LU-2040377
date created
2012-02-22 12:46:11
date last changed
2012-03-30 15:11:36
@article{2040377,
  abstract     = {Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain.
Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7\% 02 for 48 hours.
Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression.
Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca(2+) signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.},
  author       = {Gilany, Kambiz and Aerts, Maarten and Dewilde, Sylvia and Devreese, Bart and Moens, Luc and Vafakhah, Mohtaram},
  issn         = {1561-4921},
  journal      = {YAKHTEH},
  keyword      = {GeLC-MS/MS,FATTY-ACID SYNTHASE,Proteomics,Mouse,Brain,Chronic Hypoxia,GENE-EXPRESSION,METABOLIC ONCOGENE,CANCER-CELLS,PROTEINS,STRESS,OXYGEN,TOOL,REOXYGENATION,PEPTIDES},
  language     = {eng},
  number       = {4},
  pages        = {503--510},
  title        = {Proteome analysis of mouse brain exposed to chronic hypoxia},
  volume       = {12},
  year         = {2011},
}

Chicago
Gilany, Kambiz, Maarten Aerts, Sylvia Dewilde, Bart Devreese, Luc Moens, and Mohtaram Vafakhah. 2011. “Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia.” Yakhteh 12 (4): 503–510.
APA
Gilany, K., Aerts, M., Dewilde, S., Devreese, B., Moens, L., & Vafakhah, M. (2011). Proteome analysis of mouse brain exposed to chronic hypoxia. YAKHTEH, 12(4), 503–510.
Vancouver
1.
Gilany K, Aerts M, Dewilde S, Devreese B, Moens L, Vafakhah M. Proteome analysis of mouse brain exposed to chronic hypoxia. YAKHTEH. 2011;12(4):503–10.
MLA
Gilany, Kambiz, Maarten Aerts, Sylvia Dewilde, et al. “Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia.” YAKHTEH 12.4 (2011): 503–510. Print.