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Plasma serotonin and surgical small intestinal colic in horses

Sara Torfs UGent, An Maes UGent, Catherine Delesalle UGent, Siska Croubels UGent and Piet Deprez UGent (2012) European Veterinary Conference : Voorjaarsdagen, Abstracts. p.295-296
abstract
Introduction: Serotonin (5-hydroxytryptamine) is an important signaling molecule in the body and a major regulator of gastrointestinal motility. It is released in increased quantities in several conditions, including intestinal ischemia-reperfusion and platelet activation. In surgical colic cases, changes in free plasma serotonin could hypothetically contribute to the development of equine postoperative ileus. Therefore plasma serotonin concentrations were compared in healthy horses and horses undergoing laparotomy for small intestinal colic. Materials and Methods: a total of 164 EDTA plasma samples were collected from 24 control horses and 34 colic horses. Samples from the colic horses were collected at admission, immediately after surgery and at least the first morning postoperatively. Platelet stabilizing agents aspirin (1 mM), clomipramine (1 µM) and phenelzine (10 µM) were added and samples were centrifuged to produce platelet poor plasma. Serotonin concentrations were determined with a validated liquid chromatography tandem mass spectrometry method (LC-MS/MS). Serotonin differences were analyzed using logistic regression. Results: Small intestinal surgical colic cases had significantly lower plasma serotonin concentrations than healthy horses (n = 55; p < 0.001; OR 0.96; 95% CI 0.94-0.98) at admission, with a trend towards lower concentrations postoperatively (n = 55; p = 0.08) and the next morning (n = 55; p = 0.08). Within the group of colic horses, there was no difference in plasma serotonin at admission between horses that developed ileus and horses with a normal intestinal recovery (n = 27; p = 0.91), nor between horses that survived to discharge and non-survivors (n = 31; p = 0.89). Conclusions: Plasma serotonin concentrations were lower in surgical small intestinal colic horses. There was no relation to development of ileus or postoperative survival. Variable results have been published concerning serotonin values in healthy and diseased horses. More studies using standardized sampling protocols and reliable measurement methods are needed.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
colic, serotonin, horse, ELISA, LC-MS/MS
in
European Veterinary Conference : Voorjaarsdagen, Abstracts
pages
295 - 296
conference name
2012 European Veterinary Conference : Voorjaarsdagen
conference location
Amsterdam, The Netherlands
conference start
2012-04-05
conference end
2012-04-07
language
English
UGent publication?
yes
classification
C3
copyright statement
I have transferred the copyright for this publication to the publisher
id
2039089
handle
http://hdl.handle.net/1854/LU-2039089
date created
2012-02-21 14:21:12
date last changed
2012-06-12 10:56:01
@inproceedings{2039089,
  abstract     = {Introduction: Serotonin (5-hydroxytryptamine) is an important signaling molecule in the body and a major regulator of gastrointestinal motility. It is released in increased quantities in several conditions, including intestinal ischemia-reperfusion and platelet activation. In surgical colic cases, changes in free plasma serotonin could hypothetically contribute to the development of equine postoperative ileus. Therefore plasma serotonin concentrations were compared in healthy horses and horses undergoing laparotomy for small intestinal colic.
Materials and Methods: a total of 164 EDTA plasma samples were collected from 24 control horses and 34 colic horses. Samples from the colic horses were collected at admission, immediately after surgery and at least the first morning postoperatively. Platelet stabilizing agents aspirin (1 mM), clomipramine (1 {\textmu}M) and phenelzine (10 {\textmu}M) were added and samples were centrifuged to produce platelet poor plasma. Serotonin concentrations were determined with a validated liquid chromatography tandem mass spectrometry method (LC-MS/MS). Serotonin differences were analyzed using logistic regression.
Results: Small intestinal surgical colic cases had significantly lower plasma serotonin concentrations than healthy horses (n = 55; p {\textlangle} 0.001; OR 0.96; 95\% CI 0.94-0.98) at admission, with a trend towards lower concentrations postoperatively (n = 55; p = 0.08) and the next morning (n = 55; p = 0.08). Within the group of colic horses, there was no difference in plasma serotonin at admission between horses that developed ileus and horses with a normal intestinal recovery (n = 27; p = 0.91), nor between horses that survived to discharge and non-survivors (n = 31; p = 0.89). 
Conclusions: Plasma serotonin concentrations were lower in surgical small intestinal colic horses. There was no relation to development of ileus or postoperative survival. Variable results have been published concerning serotonin values in healthy and diseased horses. More studies using standardized sampling protocols and reliable measurement methods are needed.},
  author       = {Torfs, Sara and Maes, An and Delesalle, Catherine and Croubels, Siska and Deprez, Piet},
  booktitle    = {European Veterinary Conference : Voorjaarsdagen, Abstracts},
  keyword      = {colic,serotonin,horse,ELISA,LC-MS/MS},
  language     = {eng},
  location     = {Amsterdam, The Netherlands},
  pages        = {295--296},
  title        = {Plasma serotonin and surgical small intestinal colic in horses},
  year         = {2012},
}

Chicago
Torfs, Sara, An Maes, Catherine Delesalle, Siska Croubels, and Piet Deprez. 2012. “Plasma Serotonin and Surgical Small Intestinal Colic in Horses.” In European Veterinary Conference : Voorjaarsdagen, Abstracts, 295–296.
APA
Torfs, S., Maes, A., Delesalle, C., Croubels, S., & Deprez, P. (2012). Plasma serotonin and surgical small intestinal colic in horses. European Veterinary Conference : Voorjaarsdagen, Abstracts (pp. 295–296). Presented at the 2012 European Veterinary Conference : Voorjaarsdagen.
Vancouver
1.
Torfs S, Maes A, Delesalle C, Croubels S, Deprez P. Plasma serotonin and surgical small intestinal colic in horses. European Veterinary Conference : Voorjaarsdagen, Abstracts. 2012. p. 295–6.
MLA
Torfs, Sara, An Maes, Catherine Delesalle, et al. “Plasma Serotonin and Surgical Small Intestinal Colic in Horses.” European Veterinary Conference : Voorjaarsdagen, Abstracts. 2012. 295–296. Print.