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Single-step formation of degradable intracellular biomolecule microreactors

Marijke Dierendonck UGent, Stefaan De Koker UGent, Riet De Rycke UGent, Pieter Bogaert UGent, Johan Grooten UGent, Chris Vervaet UGent, Jean Paul Remon UGent and Bruno De Geest UGent (2011) ACS NANO. 5(9). p.6886-6893
abstract
Here we present a single-step all-aqueous approach to encapsulate biomolecules such as enzymes and proteins into stable microreactors. Key In this method is the use of spray-drying of the biomolecules of interest in combination with oppositely. charged,polyelectrolytes and mannitol as the sacrificial template. Remarkably, upon spray-drying in the presence of polyelectrolyte, mannitol crystallization is suppressed and the obtained amorphous mannitol offers enhanced preservation of the biomolecules' activity. Moreover, the use of mannitol allows the formation of nanopores within the microparticles upon rehydration of the microparticles in aqueous medium and subsequent dissolution, of the mannitol. The oppositely charged polyelectrolytes provide a polymeric framework which stabilizes the microparticles upon rehydration. The versatility of this approach Is demonstrated using horseradish peroxidase as the model enzyme and ovalbumin as the model antigen.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SYSTEM, SPHERES, RELEASE, DELIVERY, CAPSULES, CELLS, MICROSPHERES, PARTICLES, ANTIGEN PRESENTATION, POLYELECTROLYTE MICROCAPSULES, dendritic cells, vaccines, polyelectrolytes, drug delivery, microparticles
journal title
ACS NANO
ACS Nano
volume
5
issue
9
pages
6886 - 6893
Web of Science type
Article
Web of Science id
000295187400016
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
10.774 (2011)
JCR rank
9/229 (2011)
JCR quartile
1 (2011)
ISSN
1936-0851
DOI
10.1021/nn200901g
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2037116
handle
http://hdl.handle.net/1854/LU-2037116
date created
2012-02-17 13:19:26
date last changed
2012-06-26 14:32:34
@article{2037116,
  abstract     = {Here we present a single-step all-aqueous approach to encapsulate biomolecules such as enzymes and proteins into stable microreactors. Key In this method is the use of spray-drying of the biomolecules of interest in combination with oppositely. charged,polyelectrolytes and mannitol as the sacrificial template. Remarkably, upon spray-drying in the presence of polyelectrolyte, mannitol crystallization is suppressed and the obtained amorphous mannitol offers enhanced preservation of the biomolecules' activity. Moreover, the use of mannitol allows the formation of nanopores within the microparticles upon rehydration of the microparticles in aqueous medium and subsequent dissolution, of the mannitol. The oppositely charged polyelectrolytes provide a polymeric framework which stabilizes the microparticles upon rehydration. The versatility of this approach Is demonstrated using horseradish peroxidase as the model enzyme and ovalbumin as the model antigen.},
  author       = {Dierendonck, Marijke and De Koker, Stefaan and De Rycke, Riet and Bogaert, Pieter and Grooten, Johan and Vervaet, Chris and Remon, Jean Paul and De Geest, Bruno},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  keyword      = {SYSTEM,SPHERES,RELEASE,DELIVERY,CAPSULES,CELLS,MICROSPHERES,PARTICLES,ANTIGEN PRESENTATION,POLYELECTROLYTE MICROCAPSULES,dendritic cells,vaccines,polyelectrolytes,drug delivery,microparticles},
  language     = {eng},
  number       = {9},
  pages        = {6886--6893},
  title        = {Single-step formation of degradable intracellular biomolecule microreactors},
  url          = {http://dx.doi.org/10.1021/nn200901g},
  volume       = {5},
  year         = {2011},
}

Chicago
Dierendonck, Marijke, Stefaan De Koker, Riet De Rycke, Pieter Bogaert, Johan Grooten, Chris Vervaet, Jean Paul Remon, and Bruno De Geest. 2011. “Single-step Formation of Degradable Intracellular Biomolecule Microreactors.” Acs Nano 5 (9): 6886–6893.
APA
Dierendonck, M., De Koker, S., De Rycke, R., Bogaert, P., Grooten, J., Vervaet, C., Remon, J. P., et al. (2011). Single-step formation of degradable intracellular biomolecule microreactors. ACS NANO, 5(9), 6886–6893.
Vancouver
1.
Dierendonck M, De Koker S, De Rycke R, Bogaert P, Grooten J, Vervaet C, et al. Single-step formation of degradable intracellular biomolecule microreactors. ACS NANO. 2011;5(9):6886–93.
MLA
Dierendonck, Marijke, Stefaan De Koker, Riet De Rycke, et al. “Single-step Formation of Degradable Intracellular Biomolecule Microreactors.” ACS NANO 5.9 (2011): 6886–6893. Print.