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Single-step formation of degradable intracellular biomolecule microreactors

Marijke Dierendonck (UGent) , Stefaan De Koker (UGent) , Riet De Rycke (UGent) , Pieter Bogaert (UGent) , Johan Grooten (UGent) , Chris Vervaet (UGent) , Jean Paul Remon (UGent) and Bruno De Geest (UGent)
(2011) ACS NANO. 5(9). p.6886-6893
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Abstract
Here we present a single-step all-aqueous approach to encapsulate biomolecules such as enzymes and proteins into stable microreactors. Key In this method is the use of spray-drying of the biomolecules of interest in combination with oppositely. charged,polyelectrolytes and mannitol as the sacrificial template. Remarkably, upon spray-drying in the presence of polyelectrolyte, mannitol crystallization is suppressed and the obtained amorphous mannitol offers enhanced preservation of the biomolecules' activity. Moreover, the use of mannitol allows the formation of nanopores within the microparticles upon rehydration of the microparticles in aqueous medium and subsequent dissolution, of the mannitol. The oppositely charged polyelectrolytes provide a polymeric framework which stabilizes the microparticles upon rehydration. The versatility of this approach Is demonstrated using horseradish peroxidase as the model enzyme and ovalbumin as the model antigen.
Keywords
SYSTEM, SPHERES, RELEASE, DELIVERY, CAPSULES, CELLS, MICROSPHERES, PARTICLES, ANTIGEN PRESENTATION, POLYELECTROLYTE MICROCAPSULES, dendritic cells, vaccines, polyelectrolytes, drug delivery, microparticles

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Chicago
Dierendonck, Marijke, Stefaan De Koker, Riet De Rycke, Pieter Bogaert, Johan Grooten, Chris Vervaet, Jean Paul Remon, and Bruno De Geest. 2011. “Single-step Formation of Degradable Intracellular Biomolecule Microreactors.” Acs Nano 5 (9): 6886–6893.
APA
Dierendonck, Marijke, De Koker, S., De Rycke, R., Bogaert, P., Grooten, J., Vervaet, C., Remon, J. P., et al. (2011). Single-step formation of degradable intracellular biomolecule microreactors. ACS NANO, 5(9), 6886–6893.
Vancouver
1.
Dierendonck M, De Koker S, De Rycke R, Bogaert P, Grooten J, Vervaet C, et al. Single-step formation of degradable intracellular biomolecule microreactors. ACS NANO. 2011;5(9):6886–93.
MLA
Dierendonck, Marijke, Stefaan De Koker, Riet De Rycke, et al. “Single-step Formation of Degradable Intracellular Biomolecule Microreactors.” ACS NANO 5.9 (2011): 6886–6893. Print.
@article{2037116,
  abstract     = {Here we present a single-step all-aqueous approach to encapsulate biomolecules such as enzymes and proteins into stable microreactors. Key In this method is the use of spray-drying of the biomolecules of interest in combination with oppositely. charged,polyelectrolytes and mannitol as the sacrificial template. Remarkably, upon spray-drying in the presence of polyelectrolyte, mannitol crystallization is suppressed and the obtained amorphous mannitol offers enhanced preservation of the biomolecules' activity. Moreover, the use of mannitol allows the formation of nanopores within the microparticles upon rehydration of the microparticles in aqueous medium and subsequent dissolution, of the mannitol. The oppositely charged polyelectrolytes provide a polymeric framework which stabilizes the microparticles upon rehydration. The versatility of this approach Is demonstrated using horseradish peroxidase as the model enzyme and ovalbumin as the model antigen.},
  author       = {Dierendonck, Marijke and De Koker, Stefaan and De Rycke, Riet and Bogaert, Pieter and Grooten, Johan and Vervaet, Chris and Remon, Jean Paul and De Geest, Bruno},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  keyword      = {SYSTEM,SPHERES,RELEASE,DELIVERY,CAPSULES,CELLS,MICROSPHERES,PARTICLES,ANTIGEN PRESENTATION,POLYELECTROLYTE MICROCAPSULES,dendritic cells,vaccines,polyelectrolytes,drug delivery,microparticles},
  language     = {eng},
  number       = {9},
  pages        = {6886--6893},
  title        = {Single-step formation of degradable intracellular biomolecule microreactors},
  url          = {http://dx.doi.org/10.1021/nn200901g},
  volume       = {5},
  year         = {2011},
}

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