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Identification of compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris pv. campestris and characterization of the hypersensitive response

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Abstract
Both compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris have been identified and, for the first time, a strong hypersensitive response has been characterized. A highly reproducible mass spray inoculation protocol has been established and was used together with the more commonly used infiltration inoculation procedure to study the defense responses occurring in mature A. thaliana plants. A series of bacterial strains have been tested on A. thaliana ecotype Columbia (Col-O). X. c. pv. campestris was the most effective pathogen in these tests and was used for further detailed analysis. Several X. c. pv. campestris isolates were tested on A. thaliana Col-O, and one particular X. c. pv. campestris strain (147) was tested on 27 Arabidopsis ecotypes. Symptom development of compatible and incompatible interactions, including the hypersensitive response, was extensively characterized in A. thaliana Col-O. Lesion structure, bacterial distribution, accumulation of polyphenolic compounds, and the deposit of callose in inoculated leaves were documented by microscopic analysis. Activation of the defense-associated genes coding for phenylalanine ammonia lyase (PAL), beta-1, 3-glucanases, chitinases, and peroxidases was evaluated by Northern blot analysis.
Keywords
BIOLOGICAL FUNCTION, PATHOGENESIS-RELATED PROTEINS, GENE-EXPRESSION, AVIRULENCE GENE, PSEUDOMONAS, LOCALIZATION, METABOLISM, RESISTANCE, TOBACCO

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Chicago
Lummerzheim, Marie, Dulce De Oliveira, C Castresana, FC Miguens, E Louzada, D Roby, Marc Van Montagu, and B Timmerman. 1993. “Identification of Compatible and Incompatible Interactions Between Arabidopsis Thaliana and Xanthomonas Campestris Pv. Campestris and Characterization of the Hypersensitive Response.” Molecular Plant-microbe Interactions 6 (5): 532–544.
APA
Lummerzheim, Marie, De Oliveira, D., Castresana, C., Miguens, F., Louzada, E., Roby, D., Van Montagu, M., et al. (1993). Identification of compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris pv. campestris and characterization of the hypersensitive response. MOLECULAR PLANT-MICROBE INTERACTIONS, 6(5), 532–544.
Vancouver
1.
Lummerzheim M, De Oliveira D, Castresana C, Miguens F, Louzada E, Roby D, et al. Identification of compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris pv. campestris and characterization of the hypersensitive response. MOLECULAR PLANT-MICROBE INTERACTIONS. 1993;6(5):532–44.
MLA
Lummerzheim, Marie, Dulce De Oliveira, C Castresana, et al. “Identification of Compatible and Incompatible Interactions Between Arabidopsis Thaliana and Xanthomonas Campestris Pv. Campestris and Characterization of the Hypersensitive Response.” MOLECULAR PLANT-MICROBE INTERACTIONS 6.5 (1993): 532–544. Print.
@article{203646,
  abstract     = {Both compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris have been identified and, for the first time, a strong hypersensitive response has been characterized. A highly reproducible mass spray inoculation protocol has been established and was used together with the more commonly used infiltration inoculation procedure to study the defense responses occurring in mature A. thaliana plants. A series of bacterial strains have been tested on A. thaliana ecotype Columbia (Col-O). X. c. pv. campestris was the most effective pathogen in these tests and was used for further detailed analysis. Several X. c. pv. campestris isolates were tested on A. thaliana Col-O, and one particular X. c. pv. campestris strain (147) was tested on 27 Arabidopsis ecotypes. Symptom development of compatible and incompatible interactions, including the hypersensitive response, was extensively characterized in A. thaliana Col-O. Lesion structure, bacterial distribution, accumulation of polyphenolic compounds, and the deposit of callose in inoculated leaves were documented by microscopic analysis. Activation of the defense-associated genes coding for phenylalanine ammonia lyase (PAL), beta-1, 3-glucanases, chitinases, and peroxidases was evaluated by Northern blot analysis.},
  author       = {Lummerzheim, Marie and De Oliveira, Dulce and Castresana, C and Miguens, FC and Louzada, E and Roby, D and Van Montagu, Marc and Timmerman, B},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {BIOLOGICAL FUNCTION,PATHOGENESIS-RELATED PROTEINS,GENE-EXPRESSION,AVIRULENCE GENE,PSEUDOMONAS,LOCALIZATION,METABOLISM,RESISTANCE,TOBACCO},
  language     = {eng},
  number       = {5},
  pages        = {532--544},
  title        = {Identification of compatible and incompatible interactions between Arabidopsis thaliana and Xanthomonas campestris pv. campestris and characterization of the hypersensitive response},
  url          = {http://dx.doi.org/10.1094/MPMI-6-532},
  volume       = {6},
  year         = {1993},
}

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