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Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes

(1993) RESEARCH IN VETERINARY SCIENCE. 55(2). p.203-208
Author
Organization
Abstract
Antigenic differences between the developmental stages of Ostertagia ostertagi were studied by SDS-PAGE and Western blotting. Gel electrophoresis showed a complex protein pattern different for every stage with the O ostertagi fourth stage larvae (L4) showing an intermediate protein pattern between the third stage larvae (L3) and the adult stage. Immunoblotting showed that IgG1, IgG2 and IgM immunoglobulins present in serum from uninfected calves identified several O ostertagi antigens at every stage. When using serum from O ostertagi infected calves, O ostertagi specific IgG1 was the predominant bovine immunoglobulin. Specific IgG2 and IgM responses were also observed, while specific IgA antibodies were hardly detectable. Severe IgG1 cross reactivity was demonstrated when using anti-Cooperia oncophora serum.
Keywords
DICTYOCAULUS-VIVIPARUS, IMMUNE-RESPONSES, CALVES, CIRCUMCINCTA, IDENTIFICATION, VACCINATION, ANTIBODIES, INFECTION, EXTRACTS, RADIATUM

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Chicago
Hilderson, Hans, DC De Graaf, Jozef Vercruysse, and Paul Berghen. 1993. “Characterisation of Ostertagia Ostertagi Antigens by the Different Bovine Immunoglobulin Isotypes.” Research in Veterinary Science 55 (2): 203–208.
APA
Hilderson, H., De Graaf, D., Vercruysse, J., & Berghen, P. (1993). Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes. RESEARCH IN VETERINARY SCIENCE, 55(2), 203–208.
Vancouver
1.
Hilderson H, De Graaf D, Vercruysse J, Berghen P. Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes. RESEARCH IN VETERINARY SCIENCE. 1993;55(2):203–8.
MLA
Hilderson, Hans, DC De Graaf, Jozef Vercruysse, et al. “Characterisation of Ostertagia Ostertagi Antigens by the Different Bovine Immunoglobulin Isotypes.” RESEARCH IN VETERINARY SCIENCE 55.2 (1993): 203–208. Print.
@article{203634,
  abstract     = {Antigenic differences between the developmental stages of Ostertagia ostertagi were studied by SDS-PAGE and Western blotting. Gel electrophoresis showed a complex protein pattern different for every stage with the O ostertagi fourth stage larvae (L4) showing an intermediate protein pattern between the third stage larvae (L3) and the adult stage. Immunoblotting showed that IgG1, IgG2 and IgM immunoglobulins present in serum from uninfected calves identified several O ostertagi antigens at every stage. When using serum from O ostertagi infected calves, O ostertagi specific IgG1 was the predominant bovine immunoglobulin. Specific IgG2 and IgM responses were also observed, while specific IgA antibodies were hardly detectable. Severe IgG1 cross reactivity was demonstrated when using anti-Cooperia oncophora serum.},
  author       = {Hilderson, Hans and De Graaf, DC and Vercruysse, Jozef and Berghen, Paul},
  issn         = {0034-5288},
  journal      = {RESEARCH IN VETERINARY SCIENCE},
  keyword      = {DICTYOCAULUS-VIVIPARUS,IMMUNE-RESPONSES,CALVES,CIRCUMCINCTA,IDENTIFICATION,VACCINATION,ANTIBODIES,INFECTION,EXTRACTS,RADIATUM},
  language     = {eng},
  number       = {2},
  pages        = {203--208},
  title        = {Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes},
  url          = {http://dx.doi.org/10.1016/0034-5288(93)90082-Q},
  volume       = {55},
  year         = {1993},
}

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