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Toxicity ranking and toxic mode of action evaluation of commonly used agricultural adjuvants on the basis of bacterial gene expression profiles

Ingrid Nobels, Pieter Spanoghe UGent, Geert Haesaert UGent, Johan Robbens and Ronny Blust (2011) PLOS ONE. 6(11).
abstract
The omnipresent group of pesticide adjuvants are often referred to as "inert" ingredients, a rather misleading term since consumers associate this term with "safe". The upcoming new EU regulation concerning the introduction of plant protection products on the market (EC1107/2009) includes for the first time the demand for information on the possible negative effects of not only the active ingredients but also the used adjuvants. This new regulation requires basic toxicological information that allows decisions on the use/ban or preference of use of available adjuvants. In this study we obtained toxicological relevant information through a multiple endpoint reporter assay for a broad selection of commonly used adjuvants including several solvents (e. g. isophorone) and non-ionic surfactants (e. g. ethoxylated alcohols). The used assay allows the toxicity screening in a mechanistic way, with direct measurement of specific toxicological responses (e. g. oxidative stress, DNA damage, membrane damage and general cell lesions). The results show that the selected solvents are less toxic than the surfactants, suggesting that solvents may have a preference of use, but further research on more compounds is needed to confirm this observation. The gene expression profiles of the selected surfactants reveal that a phenol (ethoxylated tristyrylphenol) and an organosilicone surfactant (ethoxylated trisiloxane) show little or no inductions at EC(20) concentrations, making them preferred surfactants for use in different applications. The organosilicone surfactant shows little or no toxicity and good adjuvant properties. However, this study also illustrates possible genotoxicity (induction of the bacterial SOS response) for several surfactants (POEA, AE, tri-EO, EO FA and EO NP) and one solvent (gamma-butyrolactone). Although the number of compounds that were evaluated is rather limited (13), the results show that the used reporter assay is a promising tool to rank commonly used agricultural adjuvants based on toxicity and toxic mode of action data.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INGREDIENTS, MECHANISMS, ASSAY, GLYPHOSATE, ENVIRONMENTAL RISK-ASSESSMENT, PESTICIDES, SURFACTANT, TADPOLES, STRESS, PART
journal title
PLOS ONE
PLoS One
volume
6
issue
11
article_number
e24139
pages
10 pages
Web of Science type
Article
Web of Science id
000297789200002
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0024139
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2034281
handle
http://hdl.handle.net/1854/LU-2034281
date created
2012-02-15 15:59:31
date last changed
2012-03-14 14:51:51
@article{2034281,
  abstract     = {The omnipresent group of pesticide adjuvants are often referred to as {\textacutedbl}inert{\textacutedbl} ingredients, a rather misleading term since consumers associate this term with {\textacutedbl}safe{\textacutedbl}. The upcoming new EU regulation concerning the introduction of plant protection products on the market (EC1107/2009) includes for the first time the demand for information on the possible negative effects of not only the active ingredients but also the used adjuvants. This new regulation requires basic toxicological information that allows decisions on the use/ban or preference of use of available adjuvants. In this study we obtained toxicological relevant information through a multiple endpoint reporter assay for a broad selection of commonly used adjuvants including several solvents (e. g. isophorone) and non-ionic surfactants (e. g. ethoxylated alcohols). The used assay allows the toxicity screening in a mechanistic way, with direct measurement of specific toxicological responses (e. g. oxidative stress, DNA damage, membrane damage and general cell lesions). The results show that the selected solvents are less toxic than the surfactants, suggesting that solvents may have a preference of use, but further research on more compounds is needed to confirm this observation. The gene expression profiles of the selected surfactants reveal that a phenol (ethoxylated tristyrylphenol) and an organosilicone surfactant (ethoxylated trisiloxane) show little or no inductions at EC(20) concentrations, making them preferred surfactants for use in different applications. The organosilicone surfactant shows little or no toxicity and good adjuvant properties. However, this study also illustrates possible genotoxicity (induction of the bacterial SOS response) for several surfactants (POEA, AE, tri-EO, EO FA and EO NP) and one solvent (gamma-butyrolactone). Although the number of compounds that were evaluated is rather limited (13), the results show that the used reporter assay is a promising tool to rank commonly used agricultural adjuvants based on toxicity and toxic mode of action data.},
  articleno    = {e24139},
  author       = {Nobels, Ingrid and Spanoghe, Pieter and Haesaert, Geert and Robbens, Johan and Blust, Ronny},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {INGREDIENTS,MECHANISMS,ASSAY,GLYPHOSATE,ENVIRONMENTAL RISK-ASSESSMENT,PESTICIDES,SURFACTANT,TADPOLES,STRESS,PART},
  language     = {eng},
  number       = {11},
  pages        = {10},
  title        = {Toxicity ranking and toxic mode of action evaluation of commonly used agricultural adjuvants on the basis of bacterial gene expression profiles},
  url          = {http://dx.doi.org/10.1371/journal.pone.0024139},
  volume       = {6},
  year         = {2011},
}

Chicago
Nobels, Ingrid, Pieter Spanoghe, Geert Haesaert, Johan Robbens, and Ronny Blust. 2011. “Toxicity Ranking and Toxic Mode of Action Evaluation of Commonly Used Agricultural Adjuvants on the Basis of Bacterial Gene Expression Profiles.” Plos One 6 (11).
APA
Nobels, Ingrid, Spanoghe, P., Haesaert, G., Robbens, J., & Blust, R. (2011). Toxicity ranking and toxic mode of action evaluation of commonly used agricultural adjuvants on the basis of bacterial gene expression profiles. PLOS ONE, 6(11).
Vancouver
1.
Nobels I, Spanoghe P, Haesaert G, Robbens J, Blust R. Toxicity ranking and toxic mode of action evaluation of commonly used agricultural adjuvants on the basis of bacterial gene expression profiles. PLOS ONE. 2011;6(11).
MLA
Nobels, Ingrid, Pieter Spanoghe, Geert Haesaert, et al. “Toxicity Ranking and Toxic Mode of Action Evaluation of Commonly Used Agricultural Adjuvants on the Basis of Bacterial Gene Expression Profiles.” PLOS ONE 6.11 (2011): n. pag. Print.