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Depot-specific mRNA expression by equine adipocytes

Lien Bruynsteen (UGent) , Tim Erkens (UGent) , Luc Peelman (UGent) , Geert Janssens (UGent) , Patricia A Harris and Myriam Hesta (UGent)
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Abstract
Introduction: Obesity in man and horses has been described as a state of low-grade inflammation characterised by elevated concentrations of circulating inflammatory markers. In horses, adipose tissue distributed specifically on the crest of the neck could indicate or contribute to hyperinsulinemia, insulin resistance or risk for laminitis. The purpose of the study was to compare mRNA expression between different equine adipose tissue depots and the influence of body condition score, using a reliable set of reference genes. Materials and methods: From 12 healthy geldings, euthanised for non-research purposes, adipose tissue samples were collected from neck (nuchal), intra-abdominal (mesenterial, right kidney, retroperitoneal) and subcutaneous (loin, tail head) fat depots. The horses were divided into 2 groups (n=6) according to their BCS: normal BCS (4-5/9) or overweight and obese BCS (7-8.5/9). Results and discussion: Reference gene expression stability and optimal number for normalization was evaluated with the geNorm algorithm. The genes ACTB, GAPDH, HPRT, RPL 32 and SDHA were identified as reference genes. No significant effect of body condition was found for any of the parameters evaluated (P>0.05). Leptin mRNA expression was higher in the three neck samples compared to the mesenteric adipose tissue samples (P<0.05). In contrast, CCL5 and IL-10 had lower expressions in nuchal adipose tissue (P<0.05). Expression of adiponectin mRNA was lower in the tailhead region compared to the nuchal adipose tissue and peri-renal adipose tissue (P<0.05). Pro-inflammatory cytokine IL-1β mRNA expression, was lower in the loin adipose tissue compared to the peri-renal adipose tissue (P<0.05). No significant effect was found for SOD2 and MMP2 (P>0.05). Conclusions: Adipose tissue location is associated with differences in mRNA expression of the genes involved in inflammatory processes. These results suggest that individual differences in adipose tissue distribution may affect inflammatory status.
Keywords
equine adipocytes, mRNA expression, reference genes, cytokines

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MLA
Bruynsteen, Lien, Tim Erkens, Luc Peelman, et al. “Depot-specific mRNA Expression by Equine Adipocytes.” Congress Proceedings : 15th Congress European Society of Veterinary and Comparative Nutrition. Ed. Carlos Castrillo et al. Zaragoza, Spain: Universidad Zaragoza. Enivronmental Sciences Institute, 2011. 63–63. Print.
APA
Bruynsteen, L., Erkens, T., Peelman, L., Janssens, G., Harris, P. A., & Hesta, M. (2011). Depot-specific mRNA expression by equine adipocytes. In C. Castrillo, M. Latorre, M. Fondevilla, & J. Guada (Eds.), Congress proceedings : 15th congress European Society of Veterinary and Comparative Nutrition (pp. 63–63). Presented at the 15th Congress of the European Society of Veterinary and Comparative Nutrition (ESVCN 2011), Zaragoza, Spain: Universidad Zaragoza. Enivronmental Sciences Institute.
Chicago author-date
Bruynsteen, Lien, Tim Erkens, Luc Peelman, Geert Janssens, Patricia A Harris, and Myriam Hesta. 2011. “Depot-specific mRNA Expression by Equine Adipocytes.” In Congress Proceedings : 15th Congress European Society of Veterinary and Comparative Nutrition, ed. Carlos Castrillo, MA Latorre, M Fondevilla, and JA Guada, 63–63. Zaragoza, Spain: Universidad Zaragoza. Enivronmental Sciences Institute.
Chicago author-date (all authors)
Bruynsteen, Lien, Tim Erkens, Luc Peelman, Geert Janssens, Patricia A Harris, and Myriam Hesta. 2011. “Depot-specific mRNA Expression by Equine Adipocytes.” In Congress Proceedings : 15th Congress European Society of Veterinary and Comparative Nutrition, ed. Carlos Castrillo, MA Latorre, M Fondevilla, and JA Guada, 63–63. Zaragoza, Spain: Universidad Zaragoza. Enivronmental Sciences Institute.
Vancouver
1.
Bruynsteen L, Erkens T, Peelman L, Janssens G, Harris PA, Hesta M. Depot-specific mRNA expression by equine adipocytes. In: Castrillo C, Latorre M, Fondevilla M, Guada J, editors. Congress proceedings : 15th congress European Society of Veterinary and Comparative Nutrition. Zaragoza, Spain: Universidad Zaragoza. Enivronmental Sciences Institute; 2011. p. 63–63.
IEEE
[1]
L. Bruynsteen, T. Erkens, L. Peelman, G. Janssens, P. A. Harris, and M. Hesta, “Depot-specific mRNA expression by equine adipocytes,” in Congress proceedings : 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 2011, pp. 63–63.
@inproceedings{2033697,
  abstract     = {{Introduction: Obesity in man and horses has been described as a state of low-grade inflammation characterised by elevated concentrations of circulating inflammatory markers. In horses, adipose tissue distributed specifically on the crest of the neck could indicate or contribute to hyperinsulinemia, insulin resistance or risk for laminitis. The purpose of the study was to compare mRNA expression between different equine adipose tissue depots and the influence of body condition score, using a reliable set of reference genes.
Materials and methods: From 12 healthy geldings, euthanised for non-research purposes, adipose tissue samples were collected from neck (nuchal), intra-abdominal (mesenterial, right kidney, retroperitoneal) and subcutaneous (loin, tail head) fat depots. The horses were divided into 2 groups (n=6) according to their BCS: normal BCS (4-5/9) or overweight and obese BCS (7-8.5/9). 
Results and discussion: Reference gene expression stability and optimal number for normalization was evaluated with the geNorm algorithm. The genes ACTB, GAPDH, HPRT, RPL 32 and SDHA were identified as reference genes. No significant effect of body condition was found for any of the parameters evaluated (P>0.05). Leptin mRNA expression was higher in the three neck samples compared to the mesenteric adipose tissue samples (P<0.05). In contrast, CCL5 and IL-10 had lower expressions in nuchal adipose tissue (P<0.05). Expression of adiponectin mRNA was lower in the tailhead region compared to the nuchal adipose tissue and peri-renal adipose tissue (P<0.05). Pro-inflammatory cytokine IL-1β mRNA expression, was lower in the loin adipose tissue compared to the peri-renal adipose tissue (P<0.05). No significant effect was found for SOD2 and MMP2 (P>0.05).
Conclusions: Adipose tissue location is associated with differences in mRNA expression of the genes involved in inflammatory processes. These results suggest that individual differences in adipose tissue distribution may affect inflammatory status.}},
  author       = {{Bruynsteen, Lien and Erkens, Tim and Peelman, Luc and Janssens, Geert and Harris, Patricia A and Hesta, Myriam}},
  booktitle    = {{Congress proceedings : 15th congress European Society of Veterinary and Comparative Nutrition}},
  editor       = {{Castrillo, Carlos and Latorre, MA and Fondevilla, M and Guada, JA}},
  keywords     = {{equine adipocytes,mRNA expression,reference genes,cytokines}},
  language     = {{eng}},
  location     = {{Zaragoza, Spain}},
  pages        = {{63--63}},
  publisher    = {{Universidad Zaragoza. Enivronmental Sciences Institute}},
  title        = {{Depot-specific mRNA expression by equine adipocytes}},
  year         = {{2011}},
}