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Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering

(2011) BIOMACROMOLECULES. 12(4). p.851-858
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Abstract
In the present work; 3D CAD scaffolds for tissue engineering applications were developed starting from methacrylamide-modified,gelatin (GelMOD) using two-photon polymerization (2PP). The scaffolds were cross-linked employing the biocompatible photoinitiator Irgacure 2959. Because gelatin is derived. from collagen Main constituent of the ECM), the developed materials, mimic the cellular microenvironment from a chemical point of,View. In addition, by applying the 2pp technique, structural properties Of the cellular microenvironment can also be mimicked: Furthermore, in vitro degradation assays indicated that the enzymatic degradation capability of gelatin is preserved for the methacrylamide-modified derivative. An in depth morphological:analysis of the fabricated scaffolds demonstrated that the parameters of the CAD model are reproduced with great. ridge like surface topography on the order of 1.5 gm. The developed scaffolds showed an excellent stability in culture medium. In a final part of the present Work, the suitability of the developed scaffolds for tissue engineering applications was verified. The results, indicated that the applied materials are suitable to support porcine mesenchymal stem cell adhesion and subsequent proliferation.: Upon applying osteogenic stimulation, the seeded cells differentiated into the anticipated lineage. Energy dispersive X ray (EDX), analysis showed the induced calcification of the scaffold's. The results clearly indicate that 2PP is Capable of manufacturing precisely constructed 3D tissue engineering scaffolds using photosensitive polymers as staffing material.
Keywords
STRUCTURE LIBRARY, COLLAGEN SCAFFOLD, COMPUTER-AIDED-DESIGN, HYDROGELS, 2-PHOTON POLYMERIZATION, BEHAVIOR

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Chicago
Ovsianikov, Aleksandr, Andrea Deiwick, Sandra Van Vlierberghe, Peter Dubruel, Lena Möller, Gerald Dräger, and Boris Chichkov. 2011. “Laser Fabrication of Three-dimensional CAD Scaffolds from Photosensitive Gelatin for Applications in Tissue Engineering.” Biomacromolecules 12 (4): 851–858.
APA
Ovsianikov, A., Deiwick, A., Van Vlierberghe, S., Dubruel, P., Möller, L., Dräger, G., & Chichkov, B. (2011). Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering. BIOMACROMOLECULES, 12(4), 851–858.
Vancouver
1.
Ovsianikov A, Deiwick A, Van Vlierberghe S, Dubruel P, Möller L, Dräger G, et al. Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering. BIOMACROMOLECULES. 2011;12(4):851–8.
MLA
Ovsianikov, Aleksandr, Andrea Deiwick, Sandra Van Vlierberghe, et al. “Laser Fabrication of Three-dimensional CAD Scaffolds from Photosensitive Gelatin for Applications in Tissue Engineering.” BIOMACROMOLECULES 12.4 (2011): 851–858. Print.
@article{2033319,
  abstract     = {In the present work; 3D CAD scaffolds for tissue engineering applications were developed starting from methacrylamide-modified,gelatin (GelMOD) using two-photon polymerization (2PP). The scaffolds were cross-linked employing the biocompatible photoinitiator Irgacure 2959. Because gelatin is derived. from collagen Main constituent of the ECM), the developed materials, mimic the cellular microenvironment from a chemical point of,View. In addition, by applying the 2pp technique, structural properties Of the cellular microenvironment can also be mimicked: Furthermore, in vitro degradation assays indicated that the enzymatic degradation capability of gelatin is preserved for the methacrylamide-modified derivative. An in depth morphological:analysis of the fabricated scaffolds demonstrated that the parameters of the CAD model are reproduced with great. ridge like surface topography on the order of 1.5 gm. The developed scaffolds showed an excellent stability in culture medium. In a final part of the present Work, the suitability of the developed scaffolds for tissue engineering applications was verified. The results, indicated that the applied materials are suitable to support porcine mesenchymal stem cell adhesion and subsequent proliferation.: Upon applying osteogenic stimulation, the seeded cells differentiated into the anticipated lineage. Energy dispersive X ray (EDX), analysis showed the induced calcification of the scaffold's. The results clearly indicate that 2PP is Capable of manufacturing precisely constructed 3D tissue engineering scaffolds using photosensitive polymers as staffing material.},
  author       = {Ovsianikov, Aleksandr and Deiwick, Andrea and Van Vlierberghe, Sandra and Dubruel, Peter and M{\"o}ller, Lena and Dr{\"a}ger, Gerald and Chichkov, Boris},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {STRUCTURE LIBRARY,COLLAGEN SCAFFOLD,COMPUTER-AIDED-DESIGN,HYDROGELS,2-PHOTON POLYMERIZATION,BEHAVIOR},
  language     = {eng},
  number       = {4},
  pages        = {851--858},
  title        = {Laser fabrication of three-dimensional CAD scaffolds from photosensitive gelatin for applications in tissue engineering},
  url          = {http://dx.doi.org/10.1021/bm1015305},
  volume       = {12},
  year         = {2011},
}

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