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Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping

Anna Depicker UGent, Michel De Wilde, Guido De Vos, R De Vos, Marc Van Montagu UGent and Jeff Schell (1980) PLASMID. 3(2). p.193-211
abstract
The nopaline Ti-plasmid pTiC58 of Agrobacterium tumefaciens C58 was characterized by restriction endonuclease analysis. Fragments generated by HindIII (52), HpaI (17), SmaI (37), Kpn (20), BamHI (>50), and XbaI (9) were arranged into a circular map corresponding to a genome size of 132 x 10^6 D. The map was established by analysis of large overlapping plasmid segments of pTiC58 derived from a partial HindIII digest and cloned in the vector pBR322. Taken together, the segments present in the 33 hybrid plasmids obtained cover the entire pTiC58 DNA except for 2% located between map coordinates 22 and 24.7 T. The restriction map of the pTiC58 region containing the T-DNA is presented in greater detail.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
PLASMID
Plasmid
volume
3
issue
2
pages
193 - 211
Web of Science type
Article
ISSN
0147-619X
DOI
10.1016/0147-619X(80)90109-2
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2029965
handle
http://hdl.handle.net/1854/LU-2029965
date created
2012-02-14 14:52:53
date last changed
2016-12-19 15:42:03
@article{2029965,
  abstract     = {The nopaline Ti-plasmid pTiC58 of Agrobacterium tumefaciens C58 was characterized by restriction endonuclease analysis. Fragments generated by HindIII (52), HpaI (17), SmaI (37), Kpn (20), BamHI ({\textrangle}50), and XbaI (9) were arranged into a circular map corresponding to a genome size of 132 x 10\^{ }6 D. The map was established by analysis of large overlapping plasmid segments of pTiC58 derived from a partial HindIII digest and cloned in the vector pBR322. Taken together, the segments present in the 33 hybrid plasmids obtained cover the entire pTiC58 DNA except for 2\% located between map coordinates 22 and 24.7 T. The restriction map of the pTiC58 region containing the T-DNA is presented in greater detail.},
  author       = {Depicker, Anna and De Wilde, Michel and De Vos, Guido and De Vos, R and Van Montagu, Marc and Schell, Jeff},
  issn         = {0147-619X},
  journal      = {PLASMID},
  language     = {eng},
  number       = {2},
  pages        = {193--211},
  title        = {Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping},
  url          = {http://dx.doi.org/10.1016/0147-619X(80)90109-2},
  volume       = {3},
  year         = {1980},
}

Chicago
Depicker, Anna, Michel De Wilde, Guido De Vos, R De Vos, Marc Van Montagu, and Jeff Schell. 1980. “Molecular Cloning of Overlapping Segments of the Nopaline Ti-plasmid pTiC58 as a Means to Restriction Endonuclease Mapping.” Plasmid 3 (2): 193–211.
APA
Depicker, A., De Wilde, M., De Vos, G., De Vos, R., Van Montagu, M., & Schell, J. (1980). Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. PLASMID, 3(2), 193–211.
Vancouver
1.
Depicker A, De Wilde M, De Vos G, De Vos R, Van Montagu M, Schell J. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. PLASMID. 1980;3(2):193–211.
MLA
Depicker, Anna, Michel De Wilde, Guido De Vos, et al. “Molecular Cloning of Overlapping Segments of the Nopaline Ti-plasmid pTiC58 as a Means to Restriction Endonuclease Mapping.” PLASMID 3.2 (1980): 193–211. Print.