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Lyophilization of protein-loaded polyelectrolyte microcapsules

Marie-Luce De Temmerman UGent, Joanna Rejman UGent, Johan Grooten UGent, Thomas De Beer UGent, Chris Vervaet UGent, Jo Demeester UGent and Stefaan De Smedt UGent (2011) PHARMACEUTICAL RESEARCH. 28(7). p.1765-1773
abstract
Purpose To evaluate if lyophilization can be used to obtain a dry formulation of polyelectrolyte microcapsules, which have emerged as a new class of microparticles for the encapsulation and delivery of biomacromolecules. Methods Microcapsules composed of dextran sulfate and poly-L-arginine were obtained by coating CaCO(3) microparticles by means of the layer-by-layer technique. Microcapsules were lyophilized using different stabilizers; intactness was checked by CLSM and SEM. Horseradish peroxidase was encapsulated as model enzyme and retained activity after freeze-drying was determined using a fluorescence assay. Ovalbumin was encapsulated as model antigen; immunogenicity after lyophilization was evaluated in vitro by a T-cell proliferation assay and in vivo by measuring the antibody titer in mice. Results The results clearly demonstrate the necessity of using polyols in the formulation to prevent rupture of the microcapsules and to preserve the activity of encapsulated enzymes. Lyophilized microcapsules appeared as a promising adjuvant for antigen delivery, as both in vitro as in vivo assays showed higher immune activation compared to free antigen. Conclusions Lyophilization is a promising strategy towards improved stability of protein-loaded microcapsules.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHARMACEUTICALS, CAPSULES, MICROPARTICLES, drug delivery, encapsulation, layer-by-layer, lyophilization, microcapsules, MECHANICAL-PROPERTIES, MULTILAYER MICROCAPSULES, SUSTAINED-RELEASE, HOLLOW SPHERES, DRUG-RELEASE, ENCAPSULATION, PARTICLES
journal title
PHARMACEUTICAL RESEARCH
Pharm. Res.
volume
28
issue
7
pages
1765 - 1773
Web of Science type
Article
Web of Science id
000291357700027
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
4.093 (2011)
JCR rank
42/259 (2011)
JCR quartile
1 (2011)
ISSN
0724-8741
DOI
10.1007/s11095-011-0411-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2029613
handle
http://hdl.handle.net/1854/LU-2029613
date created
2012-02-14 11:21:14
date last changed
2012-03-12 16:21:29
@article{2029613,
  abstract     = {Purpose To evaluate if lyophilization can be used to obtain a dry formulation of polyelectrolyte microcapsules, which have emerged as a new class of microparticles for the encapsulation and delivery of biomacromolecules. 
Methods Microcapsules composed of dextran sulfate and poly-L-arginine were obtained by coating CaCO(3) microparticles by means of the layer-by-layer technique. Microcapsules were lyophilized using different stabilizers; intactness was checked by CLSM and SEM. Horseradish peroxidase was encapsulated as model enzyme and retained activity after freeze-drying was determined using a fluorescence assay. Ovalbumin was encapsulated as model antigen; immunogenicity after lyophilization was evaluated in vitro by a T-cell proliferation assay and in vivo by measuring the antibody titer in mice. 
Results The results clearly demonstrate the necessity of using polyols in the formulation to prevent rupture of the microcapsules and to preserve the activity of encapsulated enzymes. Lyophilized microcapsules appeared as a promising adjuvant for antigen delivery, as both in vitro as in vivo assays showed higher immune activation compared to free antigen. 
Conclusions Lyophilization is a promising strategy towards improved stability of protein-loaded microcapsules.},
  author       = {De Temmerman, Marie-Luce and Rejman, Joanna and Grooten, Johan and De Beer, Thomas and Vervaet, Chris and Demeester, Jo and De Smedt, Stefaan},
  issn         = {0724-8741},
  journal      = {PHARMACEUTICAL RESEARCH},
  keyword      = {PHARMACEUTICALS,CAPSULES,MICROPARTICLES,drug delivery,encapsulation,layer-by-layer,lyophilization,microcapsules,MECHANICAL-PROPERTIES,MULTILAYER MICROCAPSULES,SUSTAINED-RELEASE,HOLLOW SPHERES,DRUG-RELEASE,ENCAPSULATION,PARTICLES},
  language     = {eng},
  number       = {7},
  pages        = {1765--1773},
  title        = {Lyophilization of protein-loaded polyelectrolyte microcapsules},
  url          = {http://dx.doi.org/10.1007/s11095-011-0411-z},
  volume       = {28},
  year         = {2011},
}

Chicago
De Temmerman, Marie-Luce, Joanna Rejman, Johan Grooten, Thomas De Beer, Chris Vervaet, Jo Demeester, and Stefaan De Smedt. 2011. “Lyophilization of Protein-loaded Polyelectrolyte Microcapsules.” Pharmaceutical Research 28 (7): 1765–1773.
APA
De Temmerman, M.-L., Rejman, J., Grooten, J., De Beer, T., Vervaet, C., Demeester, J., & De Smedt, S. (2011). Lyophilization of protein-loaded polyelectrolyte microcapsules. PHARMACEUTICAL RESEARCH, 28(7), 1765–1773.
Vancouver
1.
De Temmerman M-L, Rejman J, Grooten J, De Beer T, Vervaet C, Demeester J, et al. Lyophilization of protein-loaded polyelectrolyte microcapsules. PHARMACEUTICAL RESEARCH. 2011;28(7):1765–73.
MLA
De Temmerman, Marie-Luce, Joanna Rejman, Johan Grooten, et al. “Lyophilization of Protein-loaded Polyelectrolyte Microcapsules.” PHARMACEUTICAL RESEARCH 28.7 (2011): 1765–1773. Print.