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Bacterial host interaction of GFP-labelled Vibrio anguillarum HI610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae

Anamaria Rekecki UGent, Rangalle Gunasekara UGent, Kristof Dierckens UGent, S Laureau, Nico Boon UGent, Herman Favoreel UGent, Maria Cornelissen UGent, Patrick Sorgeloos UGent, Richard Ducatelle UGent and Peter Bossier UGent, et al. (2012) JOURNAL OF FISH DISEASES. 35(4). p.265-273
abstract
The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V.similar to anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V.similar to anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SWIMBLADDER INFLATION, DIGESTIVE-TRACT, INTESTINAL MICROFLORA, GREEN FLUORESCENT PROTEIN, ultrastructure, microscopy, larviculture, GFP-labelled pathogen, gnotobiotic model system, COD, FRY, TURBOT, SWIM BLADDER, FISH, CHALLENGE
journal title
JOURNAL OF FISH DISEASES
J. Fish Dis.
volume
35
issue
4
pages
265 - 273
Web of Science type
Article
Web of Science id
000301343100003
JCR category
VETERINARY SCIENCES
JCR impact factor
1.591 (2012)
JCR rank
30/142 (2012)
JCR quartile
1 (2012)
ISSN
0140-7775
DOI
10.1111/j.1365-2761.2011.01342.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2029107
handle
http://hdl.handle.net/1854/LU-2029107
date created
2012-02-14 09:16:34
date last changed
2013-02-27 11:26:23
@article{2029107,
  abstract     = {The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V.similar to anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V.similar to anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.},
  author       = {Rekecki, Anamaria and Gunasekara, Rangalle and Dierckens, Kristof and Laureau, S and Boon, Nico and Favoreel, Herman and Cornelissen, Maria and Sorgeloos, Patrick and Ducatelle, Richard and Bossier, Peter and Van Den Broeck, Wim},
  issn         = {0140-7775},
  journal      = {JOURNAL OF FISH DISEASES},
  keyword      = {SWIMBLADDER INFLATION,DIGESTIVE-TRACT,INTESTINAL MICROFLORA,GREEN FLUORESCENT PROTEIN,ultrastructure,microscopy,larviculture,GFP-labelled pathogen,gnotobiotic model system,COD,FRY,TURBOT,SWIM BLADDER,FISH,CHALLENGE},
  language     = {eng},
  number       = {4},
  pages        = {265--273},
  title        = {Bacterial host interaction of GFP-labelled Vibrio anguillarum HI610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae},
  url          = {http://dx.doi.org/10.1111/j.1365-2761.2011.01342.x},
  volume       = {35},
  year         = {2012},
}

Chicago
Rekecki, Anamaria, Rangalle Gunasekara, Kristof Dierckens, S Laureau, Nico Boon, Herman Favoreel, Maria Cornelissen, et al. 2012. “Bacterial Host Interaction of GFP-labelled Vibrio Anguillarum HI610 with Gnotobiotic Sea Bass, Dicentrarchus Labrax (L.), Larvae.” Journal of Fish Diseases 35 (4): 265–273.
APA
Rekecki, A., Gunasekara, R., Dierckens, K., Laureau, S., Boon, N., Favoreel, H., Cornelissen, M., et al. (2012). Bacterial host interaction of GFP-labelled Vibrio anguillarum HI610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae. JOURNAL OF FISH DISEASES, 35(4), 265–273.
Vancouver
1.
Rekecki A, Gunasekara R, Dierckens K, Laureau S, Boon N, Favoreel H, et al. Bacterial host interaction of GFP-labelled Vibrio anguillarum HI610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae. JOURNAL OF FISH DISEASES. 2012;35(4):265–73.
MLA
Rekecki, Anamaria, Rangalle Gunasekara, Kristof Dierckens, et al. “Bacterial Host Interaction of GFP-labelled Vibrio Anguillarum HI610 with Gnotobiotic Sea Bass, Dicentrarchus Labrax (L.), Larvae.” JOURNAL OF FISH DISEASES 35.4 (2012): 265–273. Print.