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Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli

Pieter Van den Abbeele UGent, Stefaan Roos, Venessa Eeckhaut UGent, Donald A MacKenzie, Melanie Derde UGent, Willy Verstraete UGent, Massimo Marzorati UGent, Sam Possemiers UGent, Barbara Vanhoecke UGent and Filip Van Immerseel UGent, et al. (2012) MICROBIAL BIOTECHNOLOGY. 5(1). p.106-115
abstract
To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We assessed the importance of the mucosal environment in this M-SHIME (mucosal-SHIME) for the colonization of lactobacilli, a group for which the mucus binding domain was recently discovered. Whereas the two dominant resident Lactobacilli, Lactobacillus mucosae and Pediococcus acidilactici, were both present in the lumen, L. mucosae was strongly enriched in mucus. As a possible explanation, the gene encoding a mucus binding (mub) protein was detected by PCR in L. mucosae. Also the strongly adherent Lactobacillus rhamnosus GG (LGG) specifically colonized mucus upon inoculation. Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P. acidilactici. The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin). Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation. This may lead to more in vivo-like microbial communities in such dynamic, long-term in vitro simulations and allow the study of the unique mucosal microbiota in health and disease.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FECES, MICROBIAL COMMUNITY, LACTIC-ACID BACTERIA, RHAMNOSUS GG, GASTROINTESTINAL-TRACT, SP NOV., ADHESION, MUCUS, PROBIOTICS, DIVERSITY
journal title
MICROBIAL BIOTECHNOLOGY
Microb. Biotechnol.
volume
5
issue
1
pages
106 - 115
Web of Science type
Article
Web of Science id
000298098300011
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.214 (2012)
JCR rank
41/157 (2012)
JCR quartile
2 (2012)
ISSN
1751-7907
DOI
10.1111/j.1751-7915.2011.00308.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2026963
handle
http://hdl.handle.net/1854/LU-2026963
date created
2012-02-10 13:09:36
date last changed
2014-05-26 10:00:47
@article{2026963,
  abstract     = {To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We assessed the importance of the mucosal environment in this M-SHIME (mucosal-SHIME) for the colonization of lactobacilli, a group for which the mucus binding domain was recently discovered. Whereas the two dominant resident Lactobacilli, Lactobacillus mucosae and Pediococcus acidilactici, were both present in the lumen, L. mucosae was strongly enriched in mucus. As a possible explanation, the gene encoding a mucus binding (mub) protein was detected by PCR in L. mucosae. Also the strongly adherent Lactobacillus rhamnosus GG (LGG) specifically colonized mucus upon inoculation. Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P. acidilactici. The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin). Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation. This may lead to more in vivo-like microbial communities in such dynamic, long-term in vitro simulations and allow the study of the unique mucosal microbiota in health and disease.},
  author       = {Van den Abbeele, Pieter and Roos, Stefaan and Eeckhaut, Venessa and MacKenzie, Donald A and Derde, Melanie and Verstraete, Willy and Marzorati, Massimo and Possemiers, Sam and Vanhoecke, Barbara and Van Immerseel, Filip and Van de Wiele, Tom},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keyword      = {FECES,MICROBIAL COMMUNITY,LACTIC-ACID BACTERIA,RHAMNOSUS GG,GASTROINTESTINAL-TRACT,SP NOV.,ADHESION,MUCUS,PROBIOTICS,DIVERSITY},
  language     = {eng},
  number       = {1},
  pages        = {106--115},
  title        = {Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli},
  url          = {http://dx.doi.org/10.1111/j.1751-7915.2011.00308.x},
  volume       = {5},
  year         = {2012},
}

Chicago
Van den Abbeele, Pieter, Stefaan Roos, Venessa Eeckhaut, Donald A MacKenzie, Melanie Derde, Willy Verstraete, Massimo Marzorati, et al. 2012. “Incorporating a Mucosal Environment in a Dynamic Gut Model Results in a More Representative Colonization by Lactobacilli.” Microbial Biotechnology 5 (1): 106–115.
APA
Van den Abbeele, P., Roos, S., Eeckhaut, V., MacKenzie, D. A., Derde, M., Verstraete, W., Marzorati, M., et al. (2012). Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. MICROBIAL BIOTECHNOLOGY, 5(1), 106–115.
Vancouver
1.
Van den Abbeele P, Roos S, Eeckhaut V, MacKenzie DA, Derde M, Verstraete W, et al. Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. MICROBIAL BIOTECHNOLOGY. 2012;5(1):106–15.
MLA
Van den Abbeele, Pieter, Stefaan Roos, Venessa Eeckhaut, et al. “Incorporating a Mucosal Environment in a Dynamic Gut Model Results in a More Representative Colonization by Lactobacilli.” MICROBIAL BIOTECHNOLOGY 5.1 (2012): 106–115. Print.