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Exacerbation of cigarette smoke-induced pulmonary inflammation by Staphylococcus aureus enterotoxin B in mice

WOUTER HUVENNE UGent, Ellen Lanckacker UGent, Olga Krysko UGent, Ken Bracke UGent, Tine Demoor UGent, Peter W Hellings, Guy Brusselle UGent, Guy Joos UGent, Claus Bachert UGent and Tania Maes UGent (2011) RESPIRATORY RESEARCH. 12.
abstract
Background: Cigarette smoke (CS) is a major risk factor for the development of COPD. CS exposure is associated with an increased risk of bacterial colonization and respiratory tract infection, because of suppressed antibacterial activities of the immune system and delayed clearance of microbial agents from the lungs. Colonization with Staphylococcus aureus results in release of virulent enterotoxins, with superantigen activity which causes T cell activation. Objective: To study the effect of Staphylococcus aureus enterotoxin B (SEB) on CS-induced inflammation, in a mouse model of COPD. Methods: C57/Bl6 mice were exposed to CS or air for 4 weeks (5 cigarettes/exposure, 4x/day, 5 days/week). Endonasal SEB (10 mu g/ml) or saline was concomitantly applied starting from week 3, on alternate days. 24 h after the last CS and SEB exposure, mice were sacrificed and bronchoalveolar lavage (BAL) fluid and lung tissue were collected. Results: Combined exposure to CS and SEB resulted in a raised number of lymphocytes and neutrophils in BAL, as well as increased numbers of CD8(+) T lymphocytes and granulocytes in lung tissue, compared to sole CS or SEB exposure. Moreover, concomitant CS/SEB exposure induced both IL-13 mRNA expression in lungs and goblet cell hyperplasia in the airway wall. In addition, combined CS/SEB exposure stimulated the formation of dense, organized aggregates of B-and T-lymphocytes in lungs, as well as significant higher CXCL-13 (protein, mRNA) and CCL19 (mRNA) levels in lungs. Conclusions: Combined CS and SEB exposure aggravates CS-induced inflammation in mice, suggesting that Staphylococcus aureus could influence the pathogenesis of COPD.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AIRWAY INFLAMMATION, BACTERIAL SUPERANTIGEN, DEPENDENT AIRWAY, CELL-ACTIVATION, IGE-ANTIBODIES, TH17 CELLS, DISEASE, NASAL, SENSITIZATION, PATHOGENESIS
journal title
RESPIRATORY RESEARCH
Respir. Res.
volume
12
article_number
69
pages
11 pages
Web of Science type
Article
Web of Science id
000292209800001
JCR category
RESPIRATORY SYSTEM
JCR impact factor
3.36 (2011)
JCR rank
13/48 (2011)
JCR quartile
2 (2011)
ISSN
1465-993X
DOI
10.1186/1465-9921-12-69
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2026522
handle
http://hdl.handle.net/1854/LU-2026522
date created
2012-02-10 11:29:39
date last changed
2012-02-13 09:03:55
@article{2026522,
  abstract     = {Background: Cigarette smoke (CS) is a major risk factor for the development of COPD. CS exposure is associated with an increased risk of bacterial colonization and respiratory tract infection, because of suppressed antibacterial activities of the immune system and delayed clearance of microbial agents from the lungs. Colonization with Staphylococcus aureus results in release of virulent enterotoxins, with superantigen activity which causes T cell activation. 
Objective: To study the effect of Staphylococcus aureus enterotoxin B (SEB) on CS-induced inflammation, in a mouse model of COPD. 
Methods: C57/Bl6 mice were exposed to CS or air for 4 weeks (5 cigarettes/exposure, 4x/day, 5 days/week). Endonasal SEB (10 mu g/ml) or saline was concomitantly applied starting from week 3, on alternate days. 24 h after the last CS and SEB exposure, mice were sacrificed and bronchoalveolar lavage (BAL) fluid and lung tissue were collected. 
Results: Combined exposure to CS and SEB resulted in a raised number of lymphocytes and neutrophils in BAL, as well as increased numbers of CD8(+) T lymphocytes and granulocytes in lung tissue, compared to sole CS or SEB exposure. Moreover, concomitant CS/SEB exposure induced both IL-13 mRNA expression in lungs and goblet cell hyperplasia in the airway wall. In addition, combined CS/SEB exposure stimulated the formation of dense, organized aggregates of B-and T-lymphocytes in lungs, as well as significant higher CXCL-13 (protein, mRNA) and CCL19 (mRNA) levels in lungs. 
Conclusions: Combined CS and SEB exposure aggravates CS-induced inflammation in mice, suggesting that Staphylococcus aureus could influence the pathogenesis of COPD.},
  articleno    = {69},
  author       = {HUVENNE, WOUTER and Lanckacker, Ellen and Krysko, Olga and Bracke, Ken and Demoor, Tine and Hellings, Peter W and Brusselle, Guy and Joos, Guy and Bachert, Claus and Maes, Tania},
  issn         = {1465-993X},
  journal      = {RESPIRATORY RESEARCH},
  keyword      = {AIRWAY INFLAMMATION,BACTERIAL SUPERANTIGEN,DEPENDENT AIRWAY,CELL-ACTIVATION,IGE-ANTIBODIES,TH17 CELLS,DISEASE,NASAL,SENSITIZATION,PATHOGENESIS},
  language     = {eng},
  pages        = {11},
  title        = {Exacerbation of cigarette smoke-induced pulmonary inflammation by Staphylococcus aureus enterotoxin B in mice},
  url          = {http://dx.doi.org/10.1186/1465-9921-12-69},
  volume       = {12},
  year         = {2011},
}

Chicago
HUVENNE, WOUTER, Ellen Lanckacker, Olga Krysko, Ken Bracke, Tine Demoor, Peter W Hellings, Guy Brusselle, Guy Joos, Claus Bachert, and Tania Maes. 2011. “Exacerbation of Cigarette Smoke-induced Pulmonary Inflammation by Staphylococcus Aureus Enterotoxin B in Mice.” Respiratory Research 12.
APA
HUVENNE, W., Lanckacker, E., Krysko, O., Bracke, K., Demoor, T., Hellings, P. W., Brusselle, G., et al. (2011). Exacerbation of cigarette smoke-induced pulmonary inflammation by Staphylococcus aureus enterotoxin B in mice. RESPIRATORY RESEARCH, 12.
Vancouver
1.
HUVENNE W, Lanckacker E, Krysko O, Bracke K, Demoor T, Hellings PW, et al. Exacerbation of cigarette smoke-induced pulmonary inflammation by Staphylococcus aureus enterotoxin B in mice. RESPIRATORY RESEARCH. 2011;12.
MLA
HUVENNE, WOUTER, Ellen Lanckacker, Olga Krysko, et al. “Exacerbation of Cigarette Smoke-induced Pulmonary Inflammation by Staphylococcus Aureus Enterotoxin B in Mice.” RESPIRATORY RESEARCH 12 (2011): n. pag. Print.