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Dried blood spot punches for confirmation of suspected γ-hydroxybutyric acid intoxications: validation of an optimized GC-MS procedure

(2011) BIOANALYSIS. 3(20). p.2271-2281
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Abstract
Background: gamma-hydroxybutyric acid (GHB), notorious as a club-and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by 'on-spot' derivatization and ana-lysis by GC-MS. Results: A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 20-35 mu l spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2-100 mu g/ml. Conclusion: A sensitive GC-MS method for GHB ana-lysis in DBS was successfully optimized and validated. The successful ana-lysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB ana-lysis in toxicological cases.
Keywords
HUMANS, HEMATOCRIT, GHB, WHOLE-BLOOD, TANDEM MASS-SPECTROMETRY, LC-MS/MS

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Citation

Please use this url to cite or link to this publication:

Chicago
Ingels, Ann-Sofie, Peter De Paepe, Kurt Anseeuw, Diederik Van Sassenbroeck, Hugo Neels, Willy Lambert, and Christophe Stove. 2011. “Dried Blood Spot Punches for Confirmation of Suspected Γ-hydroxybutyric Acid Intoxications: Validation of an Optimized GC-MS Procedure.” Bioanalysis 3 (20): 2271–2281.
APA
Ingels, A.-S., De Paepe, P., Anseeuw, K., Van Sassenbroeck, D., Neels, H., Lambert, W., & Stove, C. (2011). Dried blood spot punches for confirmation of suspected γ-hydroxybutyric acid intoxications: validation of an optimized GC-MS procedure. BIOANALYSIS, 3(20), 2271–2281.
Vancouver
1.
Ingels A-S, De Paepe P, Anseeuw K, Van Sassenbroeck D, Neels H, Lambert W, et al. Dried blood spot punches for confirmation of suspected γ-hydroxybutyric acid intoxications: validation of an optimized GC-MS procedure. BIOANALYSIS. 2011;3(20):2271–81.
MLA
Ingels, Ann-Sofie, Peter De Paepe, Kurt Anseeuw, et al. “Dried Blood Spot Punches for Confirmation of Suspected Γ-hydroxybutyric Acid Intoxications: Validation of an Optimized GC-MS Procedure.” BIOANALYSIS 3.20 (2011): 2271–2281. Print.
@article{2025676,
  abstract     = {Background: gamma-hydroxybutyric acid (GHB), notorious as a club-and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by 'on-spot' derivatization and ana-lysis by GC-MS. Results: A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 20-35 mu l spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2-100 mu g/ml. Conclusion: A sensitive GC-MS method for GHB ana-lysis in DBS was successfully optimized and validated. The successful ana-lysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB ana-lysis in toxicological cases.},
  author       = {Ingels, Ann-Sofie and De Paepe, Peter and Anseeuw, Kurt and Van Sassenbroeck, Diederik and Neels, Hugo and Lambert, Willy and Stove, Christophe},
  issn         = {1757-6180},
  journal      = {BIOANALYSIS},
  keyword      = {HUMANS,HEMATOCRIT,GHB,WHOLE-BLOOD,TANDEM MASS-SPECTROMETRY,LC-MS/MS},
  language     = {eng},
  number       = {20},
  pages        = {2271--2281},
  title        = {Dried blood spot punches for confirmation of suspected \ensuremath{\gamma}-hydroxybutyric acid intoxications: validation of an optimized GC-MS procedure},
  url          = {http://dx.doi.org/10.4155/BIO.11.204},
  volume       = {3},
  year         = {2011},
}

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