Advanced search
Add to list

Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas aeruginosa 7NSK2

(1993) BIOMETALS. 6(2). p.85-91
Author
Organization
Abstract
Zinc concentrations ranging between 0.1 and 1 mm only slightly reduced maximal growth of wild-type Pseudomonas aeruginosa 7NSK2 in iron-limiting casamino acid medium, but had a clear negative effect on the growth of mutant MPFM1 (pyoverdin negative) and especially mutant KMPCH (pyoverdin and pyochelin negative). Production of pyoverdin by wild-type strain 7NSK2 was significantly increased in the presence of 0.5 mM zinc and could not be repressed by iron even at a concentration of 100 muM. Siderophore detection via isoelectrofocusing revealed that mutant KMPCH did not produce any siderophores, while mutant MPFM1 overproduced a siderophore with an acidic isoelectric point, most likely pyochelin. Pyochelin production by MPFM1 was stimulated by the presence of zinc in a similar way as pyoverdin for the wild-type. Analysis of outer membrane proteins revealed that three iron regulated outer membrane proteins (IROMPs) (90, 85 and 75 kDa) were induced by iron deficiency in the wild-type, while mutants were found to have altered IROMP profiles. Zinc specifically enhanced the production of a 85 kDa IROMP in 7NSK2, a 75 kDa IROMP in MPFM1 and a 90 kDa IROMP in KMPCH.
Keywords
PSEUDOMONAS-AERUGINOSA, IRON DEFICIENCY, PYOCHELIN, PYOVERDIN, ZINC, OUTER-MEMBRANE PROTEIN, PLANT-GROWTH, AZOTOBACTER-VINELANDII, ESCHERICHIA-COLI, STRAIN 7NSK2, TRANSPORT, INVOLVEMENT, PYOCHELIN, ZN-2+, SOIL

Citation

Please use this url to cite or link to this publication:

MLA
Höfte, Monica, Saskia Buysens, Nico Koedam, et al. “Zinc Affects Siderophore-mediated High Affinity Iron Uptake Systems in the Rhizosphere Pseudomonas Aeruginosa 7NSK2.” BIOMETALS 6.2 (1993): 85–91. Print.
APA
Höfte, M., Buysens, S., Koedam, N., & Cornelis, P. (1993). Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas aeruginosa 7NSK2. BIOMETALS, 6(2), 85–91.
Chicago author-date
Höfte, Monica, Saskia Buysens, Nico Koedam, and Pierre Cornelis. 1993. “Zinc Affects Siderophore-mediated High Affinity Iron Uptake Systems in the Rhizosphere Pseudomonas Aeruginosa 7NSK2.” Biometals 6 (2): 85–91.
Chicago author-date (all authors)
Höfte, Monica, Saskia Buysens, Nico Koedam, and Pierre Cornelis. 1993. “Zinc Affects Siderophore-mediated High Affinity Iron Uptake Systems in the Rhizosphere Pseudomonas Aeruginosa 7NSK2.” Biometals 6 (2): 85–91.
Vancouver
1.
Höfte M, Buysens S, Koedam N, Cornelis P. Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas aeruginosa 7NSK2. BIOMETALS. 1993;6(2):85–91.
IEEE
[1]
M. Höfte, S. Buysens, N. Koedam, and P. Cornelis, “Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas aeruginosa 7NSK2,” BIOMETALS, vol. 6, no. 2, pp. 85–91, 1993.
@article{202329,
  abstract     = {Zinc concentrations ranging between 0.1 and 1 mm only slightly reduced maximal growth of wild-type Pseudomonas aeruginosa 7NSK2 in iron-limiting casamino acid medium, but had a clear negative effect on the growth of mutant MPFM1 (pyoverdin negative) and especially mutant KMPCH (pyoverdin and pyochelin negative). Production of pyoverdin by wild-type strain 7NSK2 was significantly increased in the presence of 0.5 mM zinc and could not be repressed by iron even at a concentration of 100 muM. Siderophore detection via isoelectrofocusing revealed that mutant KMPCH did not produce any siderophores, while mutant MPFM1 overproduced a siderophore with an acidic isoelectric point, most likely pyochelin. Pyochelin production by MPFM1 was stimulated by the presence of zinc in a similar way as pyoverdin for the wild-type. Analysis of outer membrane proteins revealed that three iron regulated outer membrane proteins (IROMPs) (90, 85 and 75 kDa) were induced by iron deficiency in the wild-type, while mutants were found to have altered IROMP profiles. Zinc specifically enhanced the production of a 85 kDa IROMP in 7NSK2, a 75 kDa IROMP in MPFM1 and a 90 kDa IROMP in KMPCH.},
  author       = {Höfte, Monica and Buysens, Saskia and Koedam, Nico and Cornelis, Pierre},
  issn         = {0966-0844},
  journal      = {BIOMETALS},
  keywords     = {PSEUDOMONAS-AERUGINOSA,IRON DEFICIENCY,PYOCHELIN,PYOVERDIN,ZINC,OUTER-MEMBRANE PROTEIN,PLANT-GROWTH,AZOTOBACTER-VINELANDII,ESCHERICHIA-COLI,STRAIN 7NSK2,TRANSPORT,INVOLVEMENT,PYOCHELIN,ZN-2+,SOIL},
  language     = {eng},
  number       = {2},
  pages        = {85--91},
  title        = {Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas aeruginosa 7NSK2},
  url          = {http://dx.doi.org/10.1007/BF00140108},
  volume       = {6},
  year         = {1993},
}

Altmetric
View in Altmetric