[3H]CGP-17177 Binding to ß-adrenoceptors of intact bovine mononuclear leukocytes
- Author
- M Vermeir, Eddy Roets and Christian Paul G. Burvenich (UGent)
- Organization
- Abstract
- Saturation and inhibition binding studies, using [H-3]CGP-12177 as radioligand, reveal the presence of a single class of beta-adrenoceptors on intact bovine mononuclear leukocytes. Receptor binding is inhibited by beta-adrenergic agonists with a rank order of potency (-)-isoproterenol > (-)-epinephrine > (-)-norepinephrine, indicating that the receptors are of the beta2-subtype. By Scatchard analysis, the mean receptor density was 707+/-168 receptors/cell and the dissociation constant was 0.89+/-0.47 nM. Specific binding was very high and amounted to 80-90 %. It is concluded that [H-3]CGP-12177 is a promising compound for the reliable determination of the binding characteristics of bovine mononuclear leukocytes.
- Keywords
- DENSITY, HUMAN-LYMPHOCYTES, CELLS, EXERCISE, BETA-2-ADRENOCEPTORS, NUMBER, AFFINITY, ADRENERGIC RECEPTORS
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-201838
- MLA
- Vermeir, M., et al. “[3H]CGP-17177 Binding to ß-Adrenoceptors of Intact Bovine Mononuclear Leukocytes.” ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE, vol. 321, 1993, pp. 103–11.
- APA
- Vermeir, M., Roets, E., & Burvenich, C. P. G. (1993). [3H]CGP-17177 Binding to ß-adrenoceptors of intact bovine mononuclear leukocytes. ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE, 321, 103–111.
- Chicago author-date
- Vermeir, M, Eddy Roets, and Christian Paul G. Burvenich. 1993. “[3H]CGP-17177 Binding to ß-Adrenoceptors of Intact Bovine Mononuclear Leukocytes.” ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE 321: 103–11.
- Chicago author-date (all authors)
- Vermeir, M, Eddy Roets, and Christian Paul G. Burvenich. 1993. “[3H]CGP-17177 Binding to ß-Adrenoceptors of Intact Bovine Mononuclear Leukocytes.” ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE 321: 103–111.
- Vancouver
- 1.Vermeir M, Roets E, Burvenich CPG. [3H]CGP-17177 Binding to ß-adrenoceptors of intact bovine mononuclear leukocytes. ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE. 1993;321:103–11.
- IEEE
- [1]M. Vermeir, E. Roets, and C. P. G. Burvenich, “[3H]CGP-17177 Binding to ß-adrenoceptors of intact bovine mononuclear leukocytes,” ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE, vol. 321, pp. 103–111, 1993.
@article{201838,
abstract = {{Saturation and inhibition binding studies, using [H-3]CGP-12177 as radioligand, reveal the presence of a single class of beta-adrenoceptors on intact bovine mononuclear leukocytes. Receptor binding is inhibited by beta-adrenergic agonists with a rank order of potency (-)-isoproterenol > (-)-epinephrine > (-)-norepinephrine, indicating that the receptors are of the beta2-subtype. By Scatchard analysis, the mean receptor density was 707+/-168 receptors/cell and the dissociation constant was 0.89+/-0.47 nM. Specific binding was very high and amounted to 80-90 %.
It is concluded that [H-3]CGP-12177 is a promising compound for the reliable determination of the binding characteristics of bovine mononuclear leukocytes.}},
author = {{Vermeir, M and Roets, Eddy and Burvenich, Christian Paul G.}},
issn = {{0003-9780}},
journal = {{ARCHIVES INTERNATIONALES DE PHARMACODYNAMIE ET DE THERAPIE}},
keywords = {{DENSITY,HUMAN-LYMPHOCYTES,CELLS,EXERCISE,BETA-2-ADRENOCEPTORS,NUMBER,AFFINITY,ADRENERGIC RECEPTORS}},
language = {{eng}},
pages = {{103--111}},
title = {{[3H]CGP-17177 Binding to ß-adrenoceptors of intact bovine mononuclear leukocytes}},
volume = {{321}},
year = {{1993}},
}