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Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids

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Abstract
While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.
Keywords
SIZE, particle size, single particle tracking, DELIVERY, fluorescence microscopy, nanobiophotonics, nanoparticles, nanomedicines

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Chicago
Braeckmans, Kevin, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, et al. 2011. “Fluorescence Single Particle Tracking for Sizing of Nanoparticles in Undiluted Biological Fluids.” In Proceedings of SPIE, the International Society for Optical Engineering, ed. AN Cartwright and DV Nicolau. Vol. 7908. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
APA
Braeckmans, Kevin, Buyens, K., Bouquet-Geerardyn, W., Vervaet, C., Joye, P., De Vos, F., Plawinski, L., et al. (2011). Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids. In AN Cartwright & D. Nicolau (Eds.), Proceedings of SPIE, the International Society for Optical Engineering (Vol. 7908). Presented at the Conference on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
Vancouver
1.
Braeckmans K, Buyens K, Bouquet-Geerardyn W, Vervaet C, Joye P, De Vos F, et al. Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids. In: Cartwright A, Nicolau D, editors. Proceedings of SPIE, the International Society for Optical Engineering. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering; 2011.
MLA
Braeckmans, Kevin, Kevin Buyens, Wim Bouquet-Geerardyn, et al. “Fluorescence Single Particle Tracking for Sizing of Nanoparticles in Undiluted Biological Fluids.” Proceedings of SPIE, the International Society for Optical Engineering. Ed. AN Cartwright & DV Nicolau. Vol. 7908. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering, 2011. Print.
@inproceedings{2018197,
  abstract     = {While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.},
  articleno    = {79080B},
  author       = {Braeckmans, Kevin and Buyens, Kevin and Bouquet-Geerardyn, Wim and Vervaet, Chris and Joye, Philippe and De Vos, Filip and Plawinski, Laurent and Doeuvre, Lo{\"i}c and Angles-Cano, Eduardo and Sanders, Niek and Demeester, Jo and De Smedt, Stefaan},
  booktitle    = {Proceedings of SPIE, the International Society for Optical Engineering},
  editor       = {Cartwright, AN and Nicolau, DV},
  isbn         = {9780819484451},
  issn         = {0277-786X},
  keyword      = {SIZE,particle size,single particle tracking,DELIVERY,fluorescence microscopy,nanobiophotonics,nanoparticles,nanomedicines},
  language     = {eng},
  location     = {San Francisco, CA, USA},
  pages        = {8},
  publisher    = {SPIE, the International Society for Optical Engineering},
  title        = {Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids},
  url          = {http://dx.doi.org/10.1117/12.889218},
  volume       = {7908},
  year         = {2011},
}

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