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Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids

Kevin Braeckmans UGent, Kevin Buyens UGent, Wim Bouquet-Geerardyn, Chris Vervaet UGent, Philippe Joye UGent, Filip De Vos UGent, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek Sanders UGent, et al. (2011) Proceedings of SPIE, the International Society for Optical Engineering. 7908.
abstract
While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
SIZE, particle size, single particle tracking, DELIVERY, fluorescence microscopy, nanobiophotonics, nanoparticles, nanomedicines
in
Proceedings of SPIE, the International Society for Optical Engineering
Proc. SPIE Int. Soc. Opt. Eng.
editor
AN Cartwright and DV Nicolau
volume
7908
issue title
Nanoscale imaging, sensing, and actuation for biomedical applications VIII
article number
79080B
pages
8 pages
publisher
SPIE, the International Society for Optical Engineering
place of publication
Bellingham, WA, USA
conference name
Conference on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
conference location
San Francisco, CA, USA
conference start
2011-01-24
conference end
2011-01-27
Web of Science type
Proceedings Paper
Web of Science id
000297728100008
ISSN
0277-786X
ISBN
9780819484451
DOI
10.1117/12.889218
language
English
UGent publication?
yes
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2018197
handle
http://hdl.handle.net/1854/LU-2018197
date created
2012-02-03 11:47:42
date last changed
2017-01-02 09:53:00
@inproceedings{2018197,
  abstract     = {While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.},
  articleno    = {79080B},
  author       = {Braeckmans, Kevin and Buyens, Kevin and Bouquet-Geerardyn, Wim and Vervaet, Chris and Joye, Philippe and De Vos, Filip and Plawinski, Laurent and Doeuvre, Lo{\"i}c and Angles-Cano, Eduardo and Sanders, Niek and Demeester, Jo and De Smedt, Stefaan},
  booktitle    = {Proceedings of SPIE, the International Society for Optical Engineering},
  editor       = {Cartwright, AN and Nicolau, DV},
  isbn         = {9780819484451},
  issn         = {0277-786X},
  keyword      = {SIZE,particle size,single particle tracking,DELIVERY,fluorescence microscopy,nanobiophotonics,nanoparticles,nanomedicines},
  language     = {eng},
  location     = {San Francisco, CA, USA},
  pages        = {8},
  publisher    = {SPIE, the International Society for Optical Engineering},
  title        = {Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids},
  url          = {http://dx.doi.org/10.1117/12.889218},
  volume       = {7908},
  year         = {2011},
}

Chicago
Braeckmans, Kevin, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, et al. 2011. “Fluorescence Single Particle Tracking for Sizing of Nanoparticles in Undiluted Biological Fluids.” In Proceedings of SPIE, the International Society for Optical Engineering, ed. AN Cartwright and DV Nicolau. Vol. 7908. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
APA
Braeckmans, Kevin, Buyens, K., Bouquet-Geerardyn, W., Vervaet, C., Joye, P., De Vos, F., Plawinski, L., et al. (2011). Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids. In A. Cartwright & D. Nicolau (Eds.), Proceedings of SPIE, the International Society for Optical Engineering (Vol. 7908). Presented at the Conference on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
Vancouver
1.
Braeckmans K, Buyens K, Bouquet-Geerardyn W, Vervaet C, Joye P, De Vos F, et al. Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids. In: Cartwright A, Nicolau D, editors. Proceedings of SPIE, the International Society for Optical Engineering. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering; 2011.
MLA
Braeckmans, Kevin, Kevin Buyens, Wim Bouquet-Geerardyn, et al. “Fluorescence Single Particle Tracking for Sizing of Nanoparticles in Undiluted Biological Fluids.” Proceedings of SPIE, the International Society for Optical Engineering. Ed. AN Cartwright & DV Nicolau. Vol. 7908. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering, 2011. Print.