Ghent University Academic Bibliography

Advanced

Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men

Hélène De Naeyer UGent, D Margriet Ouwens, Yves Van Nieuwenhove UGent, Piet Pattyn UGent, Leen M 't Hart, Jean Kaufman UGent, Henrike Sell, Juergen Eckel, Claude Cuvelier UGent and Youri Taes UGent, et al. (2011) OBESITY FACTS. 4(5). p.407-416
abstract
Aims: Lipotoxicity in obesity might be a failure of adipocytes to respond sufficiently adequate to persistent energy surplus. To evaluate the role of lipolytic enzymes or mitochondria in lipotoxicity, we studied expression levels of genes and proteins involved in lipolysis and mitochondrial DNA (mtDNA) content. Methods: As differences in lipid metabolism between men and women are extremely complex, we recruited only men (lean and morbidly obese) and collected subcutaneous and visceral adipose tissue during abdominal surgery for real-time PCR gene expression, protein expression, and microscopic study. Results: Although mRNA levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in visceral adipose tissue of morbidly obese men, this was not paralleled by alterations in protein expression and phosphorylation of HSL and ATGL. mtDNA content of visceral adipose tissue was increased in morbidly obese men as compared to lean controls (p < 0.013). Positive correlations were observed between visceral adipocyte size and serum triacylglycerol (r = 0.6, p < 0.007) as well as between visceral adipocyte size and CRP (r = 0.6, p < 0.009) in analyses performed separately in obese men. Conclusion: Lipotoxicity of morbidly obese men might be related to the quantitative impact of the visceral fat depot rather than to important dysregulation of involved lipolytic enzymes or adipocyte mitochondria.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle
publication status
published
subject
keyword
Hormone-sensitive lipase, Mitochondrial content, Adipose triglyceride lipase, Obesity, Lipolysis, Metabolic diseases, INSULIN-RESISTANCE, DECREASED EXPRESSION, DIABETES-MELLITUS, BODY-FAT, LIPOLYSIS, PERILIPIN, HUMANS, METABOLISM, NUMBER, WOMEN
journal title
OBESITY FACTS
Obes. Facts
volume
4
issue
5
pages
407 - 416
Web of Science type
Article
Web of Science id
000296800000012
JCR category
NUTRITION & DIETETICS
JCR impact factor
1.856 (2011)
JCR rank
42/71 (2011)
JCR quartile
3 (2011)
ISSN
1662-4025
DOI
10.1159/000333445
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2017878
handle
http://hdl.handle.net/1854/LU-2017878
date created
2012-02-03 10:51:55
date last changed
2012-02-27 15:34:52
@article{2017878,
  abstract     = {Aims: Lipotoxicity in obesity might be a failure of adipocytes to respond sufficiently adequate to persistent energy surplus. To evaluate the role of lipolytic enzymes or mitochondria in lipotoxicity, we studied expression levels of genes and proteins involved in lipolysis and mitochondrial DNA (mtDNA) content. Methods: As differences in lipid metabolism between men and women are extremely complex, we recruited only men (lean and morbidly obese) and collected subcutaneous and visceral adipose tissue during abdominal surgery for real-time PCR gene expression, protein expression, and microscopic study. Results: Although mRNA levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in visceral adipose tissue of morbidly obese men, this was not paralleled by alterations in protein expression and phosphorylation of HSL and ATGL. mtDNA content of visceral adipose tissue was increased in morbidly obese men as compared to lean controls (p {\textlangle} 0.013). Positive correlations were observed between visceral adipocyte size and serum triacylglycerol (r = 0.6, p {\textlangle} 0.007) as well as between visceral adipocyte size and CRP (r = 0.6, p {\textlangle} 0.009) in analyses performed separately in obese men. Conclusion: Lipotoxicity of morbidly obese men might be related to the quantitative impact of the visceral fat depot rather than to important dysregulation of involved lipolytic enzymes or adipocyte mitochondria.},
  author       = {De Naeyer, H{\'e}l{\`e}ne and Ouwens, D Margriet and Van Nieuwenhove, Yves and Pattyn, Piet and 't Hart, Leen M and Kaufman, Jean and Sell, Henrike and Eckel, Juergen and Cuvelier, Claude and Taes, Youri and Ruige, Johannes},
  issn         = {1662-4025},
  journal      = {OBESITY FACTS},
  keyword      = {Hormone-sensitive lipase,Mitochondrial content,Adipose triglyceride lipase,Obesity,Lipolysis,Metabolic diseases,INSULIN-RESISTANCE,DECREASED EXPRESSION,DIABETES-MELLITUS,BODY-FAT,LIPOLYSIS,PERILIPIN,HUMANS,METABOLISM,NUMBER,WOMEN},
  language     = {eng},
  number       = {5},
  pages        = {407--416},
  title        = {Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men},
  url          = {http://dx.doi.org/10.1159/000333445},
  volume       = {4},
  year         = {2011},
}

Chicago
De Naeyer, Hélène, D Margriet Ouwens, Yves Van Nieuwenhove, Piet Pattyn, Leen M ’t Hart, Jean Kaufman, Henrike Sell, et al. 2011. “Combined Gene and Protein Expression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase, Mitochondrial Content, and Adipocyte Size in Subcutaneous and Visceral Adipose Tissue of Morbidly Obese Men.” Obesity Facts 4 (5): 407–416.
APA
De Naeyer, H., Ouwens, D. M., Van Nieuwenhove, Y., Pattyn, P., ’t Hart, L. M., Kaufman, J., Sell, H., et al. (2011). Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men. OBESITY FACTS, 4(5), 407–416.
Vancouver
1.
De Naeyer H, Ouwens DM, Van Nieuwenhove Y, Pattyn P, ’t Hart LM, Kaufman J, et al. Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men. OBESITY FACTS. 2011;4(5):407–16.
MLA
De Naeyer, Hélène, D Margriet Ouwens, Yves Van Nieuwenhove, et al. “Combined Gene and Protein Expression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase, Mitochondrial Content, and Adipocyte Size in Subcutaneous and Visceral Adipose Tissue of Morbidly Obese Men.” OBESITY FACTS 4.5 (2011): 407–416. Print.