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Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men

(2011) OBESITY FACTS. 4(5). p.407-416
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Organization
Abstract
Aims: Lipotoxicity in obesity might be a failure of adipocytes to respond sufficiently adequate to persistent energy surplus. To evaluate the role of lipolytic enzymes or mitochondria in lipotoxicity, we studied expression levels of genes and proteins involved in lipolysis and mitochondrial DNA (mtDNA) content. Methods: As differences in lipid metabolism between men and women are extremely complex, we recruited only men (lean and morbidly obese) and collected subcutaneous and visceral adipose tissue during abdominal surgery for real-time PCR gene expression, protein expression, and microscopic study. Results: Although mRNA levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in visceral adipose tissue of morbidly obese men, this was not paralleled by alterations in protein expression and phosphorylation of HSL and ATGL. mtDNA content of visceral adipose tissue was increased in morbidly obese men as compared to lean controls (p < 0.013). Positive correlations were observed between visceral adipocyte size and serum triacylglycerol (r = 0.6, p < 0.007) as well as between visceral adipocyte size and CRP (r = 0.6, p < 0.009) in analyses performed separately in obese men. Conclusion: Lipotoxicity of morbidly obese men might be related to the quantitative impact of the visceral fat depot rather than to important dysregulation of involved lipolytic enzymes or adipocyte mitochondria.
Keywords
Mitochondrial content, Hormone-sensitive lipase, Adipose triglyceride lipase, Obesity, Lipolysis, Metabolic diseases, INSULIN-RESISTANCE, DECREASED EXPRESSION, DIABETES-MELLITUS, BODY-FAT, LIPOLYSIS, PERILIPIN, HUMANS, METABOLISM, NUMBER, WOMEN

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MLA
De Naeyer, Hélène, D Margriet Ouwens, Yves Van Nieuwenhove, et al. “Combined Gene and Protein Expression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase, Mitochondrial Content, and Adipocyte Size in Subcutaneous and Visceral Adipose Tissue of Morbidly Obese Men.” OBESITY FACTS 4.5 (2011): 407–416. Print.
APA
De Naeyer, H., Ouwens, D. M., Van Nieuwenhove, Y., Pattyn, P., ’t Hart, L. M., Kaufman, J., Sell, H., et al. (2011). Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men. OBESITY FACTS, 4(5), 407–416.
Chicago author-date
De Naeyer, Hélène, D Margriet Ouwens, Yves Van Nieuwenhove, Piet Pattyn, Leen M ’t Hart, Jean Kaufman, Henrike Sell, et al. 2011. “Combined Gene and Protein Expression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase, Mitochondrial Content, and Adipocyte Size in Subcutaneous and Visceral Adipose Tissue of Morbidly Obese Men.” Obesity Facts 4 (5): 407–416.
Chicago author-date (all authors)
De Naeyer, Hélène, D Margriet Ouwens, Yves Van Nieuwenhove, Piet Pattyn, Leen M ’t Hart, Jean Kaufman, Henrike Sell, Juergen Eckel, Claude Cuvelier, Youri Taes, and Johannes Ruige. 2011. “Combined Gene and Protein Expression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase, Mitochondrial Content, and Adipocyte Size in Subcutaneous and Visceral Adipose Tissue of Morbidly Obese Men.” Obesity Facts 4 (5): 407–416.
Vancouver
1.
De Naeyer H, Ouwens DM, Van Nieuwenhove Y, Pattyn P, ’t Hart LM, Kaufman J, et al. Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men. OBESITY FACTS. 2011;4(5):407–16.
IEEE
[1]
H. De Naeyer et al., “Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men,” OBESITY FACTS, vol. 4, no. 5, pp. 407–416, 2011.
@article{2017878,
  abstract     = {Aims: Lipotoxicity in obesity might be a failure of adipocytes to respond sufficiently adequate to persistent energy surplus. To evaluate the role of lipolytic enzymes or mitochondria in lipotoxicity, we studied expression levels of genes and proteins involved in lipolysis and mitochondrial DNA (mtDNA) content. Methods: As differences in lipid metabolism between men and women are extremely complex, we recruited only men (lean and morbidly obese) and collected subcutaneous and visceral adipose tissue during abdominal surgery for real-time PCR gene expression, protein expression, and microscopic study. Results: Although mRNA levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in visceral adipose tissue of morbidly obese men, this was not paralleled by alterations in protein expression and phosphorylation of HSL and ATGL. mtDNA content of visceral adipose tissue was increased in morbidly obese men as compared to lean controls (p < 0.013). Positive correlations were observed between visceral adipocyte size and serum triacylglycerol (r = 0.6, p < 0.007) as well as between visceral adipocyte size and CRP (r = 0.6, p < 0.009) in analyses performed separately in obese men. Conclusion: Lipotoxicity of morbidly obese men might be related to the quantitative impact of the visceral fat depot rather than to important dysregulation of involved lipolytic enzymes or adipocyte mitochondria.},
  author       = {De Naeyer, Hélène and Ouwens, D Margriet and Van Nieuwenhove, Yves and Pattyn, Piet and 't Hart, Leen M and Kaufman, Jean and Sell, Henrike and Eckel, Juergen and Cuvelier, Claude and Taes, Youri and Ruige, Johannes},
  issn         = {1662-4025},
  journal      = {OBESITY FACTS},
  keywords     = {Mitochondrial content,Hormone-sensitive lipase,Adipose triglyceride lipase,Obesity,Lipolysis,Metabolic diseases,INSULIN-RESISTANCE,DECREASED EXPRESSION,DIABETES-MELLITUS,BODY-FAT,LIPOLYSIS,PERILIPIN,HUMANS,METABOLISM,NUMBER,WOMEN},
  language     = {eng},
  number       = {5},
  pages        = {407--416},
  title        = {Combined gene and protein expression of hormone-sensitive lipase and adipose triglyceride lipase, mitochondrial content, and adipocyte size in subcutaneous and visceral adipose tissue of morbidly obese men},
  url          = {http://dx.doi.org/10.1159/000333445},
  volume       = {4},
  year         = {2011},
}

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