Advanced search
1 file | 775.67 KB

Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment

(2012) FEMS MICROBIOLOGY ECOLOGY. 79(3). p.685-696
Author
Organization
Abstract
The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.
Keywords
REAL-TIME PCR, MUCOSAL FLORA, LACTIC-ACID BACTERIA, SHIME, DIVERSITY, CROHNS-DISEASE, adhesion, inflammatory bowel disease, Clostridium, MAMC, INFLAMMATORY-BOWEL-DISEASE, dysbiosis, GRADIENT GEL-ELECTROPHORESIS, INTESTINAL MICROBIAL ECOSYSTEM, BUTYRATE-PRODUCING BACTERIA, GASTROINTESTINAL-TRACT

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 775.67 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Vermeiren, Joan, Pieter Van den Abbeele, Debby Laukens, Louise Kristine Vigsnæs, Martine De Vos, Nico Boon, and Tom Van de Wiele. 2012. “Decreased Colonization of Fecal Clostridium coccoides/Eubacterium Rectale Species from Ulcerative Colitis Patients in an in Vitro Dynamic Gut Model with Mucin Environment.” Fems Microbiology Ecology 79 (3): 685–696.
APA
Vermeiren, J., Van den Abbeele, P., Laukens, D., Vigsnæs, L. K., De Vos, M., Boon, N., & Van de Wiele, T. (2012). Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. FEMS MICROBIOLOGY ECOLOGY, 79(3), 685–696.
Vancouver
1.
Vermeiren J, Van den Abbeele P, Laukens D, Vigsnæs LK, De Vos M, Boon N, et al. Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. FEMS MICROBIOLOGY ECOLOGY. 2012;79(3):685–96.
MLA
Vermeiren, Joan, Pieter Van den Abbeele, Debby Laukens, et al. “Decreased Colonization of Fecal Clostridium coccoides/Eubacterium Rectale Species from Ulcerative Colitis Patients in an in Vitro Dynamic Gut Model with Mucin Environment.” FEMS MICROBIOLOGY ECOLOGY 79.3 (2012): 685–696. Print.
@article{2017155,
  abstract     = {The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.},
  author       = {Vermeiren, Joan and Van den Abbeele, Pieter and Laukens, Debby and Vigsn{\ae}s, Louise Kristine and De Vos, Martine and Boon, Nico and Van de Wiele, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {REAL-TIME PCR,MUCOSAL FLORA,LACTIC-ACID BACTERIA,SHIME,DIVERSITY,CROHNS-DISEASE,adhesion,inflammatory bowel disease,Clostridium,MAMC,INFLAMMATORY-BOWEL-DISEASE,dysbiosis,GRADIENT GEL-ELECTROPHORESIS,INTESTINAL MICROBIAL ECOSYSTEM,BUTYRATE-PRODUCING BACTERIA,GASTROINTESTINAL-TRACT},
  language     = {eng},
  number       = {3},
  pages        = {685--696},
  title        = {Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2011.01252.x},
  volume       = {79},
  year         = {2012},
}

Altmetric
View in Altmetric
Web of Science
Times cited: