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Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment

Joan Vermeiren UGent, Pieter Van den Abbeele UGent, Debby Laukens UGent, Louise Kristine Vigsnæs, Martine De Vos UGent, Nico Boon UGent and Tom Vandewiele UGent (2012) FEMS MICROBIOLOGY ECOLOGY. 79(3). p.685-696
abstract
The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SHIME, DIVERSITY, CROHNS-DISEASE, inflammatory bowel disease, adhesion, MAMC, Clostridium, INFLAMMATORY-BOWEL-DISEASE, dysbiosis, INTESTINAL MICROBIAL ECOSYSTEM, GRADIENT GEL-ELECTROPHORESIS, REAL-TIME PCR, LACTIC-ACID BACTERIA, MUCOSAL FLORA, BUTYRATE-PRODUCING BACTERIA, GASTROINTESTINAL-TRACT
journal title
FEMS MICROBIOLOGY ECOLOGY
FEMS Microbiol. Ecol.
volume
79
issue
3
pages
685 - 696
Web of Science type
Article
Web of Science id
000299257300011
JCR category
MICROBIOLOGY
JCR impact factor
3.563 (2012)
JCR rank
31/116 (2012)
JCR quartile
2 (2012)
ISSN
0168-6496
DOI
10.1111/j.1574-6941.2011.01252.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2017155
handle
http://hdl.handle.net/1854/LU-2017155
date created
2012-02-02 19:34:33
date last changed
2013-05-31 09:49:12
@article{2017155,
  abstract     = {The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.},
  author       = {Vermeiren, Joan and Van den Abbeele, Pieter and Laukens, Debby and Vigsn{\ae}s, Louise Kristine and De Vos, Martine and Boon, Nico and Vandewiele, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {SHIME,DIVERSITY,CROHNS-DISEASE,inflammatory bowel disease,adhesion,MAMC,Clostridium,INFLAMMATORY-BOWEL-DISEASE,dysbiosis,INTESTINAL MICROBIAL ECOSYSTEM,GRADIENT GEL-ELECTROPHORESIS,REAL-TIME PCR,LACTIC-ACID BACTERIA,MUCOSAL FLORA,BUTYRATE-PRODUCING BACTERIA,GASTROINTESTINAL-TRACT},
  language     = {eng},
  number       = {3},
  pages        = {685--696},
  title        = {Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2011.01252.x},
  volume       = {79},
  year         = {2012},
}

Chicago
Vermeiren, Joan, Pieter Van den Abbeele, Debby Laukens, Louise Kristine Vigsnæs, Martine De Vos, Nico Boon, and Tom Vandewiele. 2012. “Decreased Colonization of Fecal Clostridium coccoides/Eubacterium Rectale Species from Ulcerative Colitis Patients in an in Vitro Dynamic Gut Model with Mucin Environment.” Fems Microbiology Ecology 79 (3): 685–696.
APA
Vermeiren, J., Van den Abbeele, P., Laukens, D., Vigsnæs, L. K., De Vos, M., Boon, N., & Vandewiele, T. (2012). Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. FEMS MICROBIOLOGY ECOLOGY, 79(3), 685–696.
Vancouver
1.
Vermeiren J, Van den Abbeele P, Laukens D, Vigsnæs LK, De Vos M, Boon N, et al. Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. FEMS MICROBIOLOGY ECOLOGY. 2012;79(3):685–96.
MLA
Vermeiren, Joan, Pieter Van den Abbeele, Debby Laukens, et al. “Decreased Colonization of Fecal Clostridium coccoides/Eubacterium Rectale Species from Ulcerative Colitis Patients in an in Vitro Dynamic Gut Model with Mucin Environment.” FEMS MICROBIOLOGY ECOLOGY 79.3 (2012): 685–696. Print.