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Kinetics of butyrate metabolism in the normal colon and in ulcerative colitis: the effects of substrate concentration and carnitine on the b-oxidation pathway

V De Preter, Karen Geboes UGent, V Bulteel, G Vandermeulen, P Suenaert, P Rutgeerts and K Verbeke (2011) ALIMENTARY PHARMACOLOGY & THERAPEUTICS. 34(5). p.526-532
abstract
Background : Butyrate, a colonic metabolite of carbohydrates, is considered as the major energy source for the colonic mucosa. An impaired butyrate metabolism has been reported in ulcerative colitis (UC), however, the cause still remains unknown. Aim : In the present study, we investigated whether higher butyrate concentrations could normalise the oxidation rate in UC. Furthermore, it was investigated whether carnitine could enhance the butyrate oxidation. Methods : Mucosal biopsies from a total of 26 UC patients and 25 controls were incubated with (14)C-labelled Na-butyrate and the produced (14)CO(2) was measured. First, the rate of oxidative metabolism was compared at three different concentrations of Na-butyrate (0.05 mM, 1 mM and 10 mM). Then, incubations of biopsies were performed with carnitine alone or combined with ATP. Results : Overall, butyrate oxidation in UC was significantly lower than that in controls. The maximum rate of butyrate oxidation was achieved in UC and control subjects from 1 mM onwards. Increasing the butyrate concentration to a level to be present in the colonic lumen, i.e. 10 mM, did not increase the rate of butyrate oxidation in UC to the rate observed in controls. Addition of carnitine alone or combined with ATP caused no effects. Conclusions : Saturation of butyrate kinetics was achieved from 1 mM in UC and control subjects. The rate of butyrate metabolism was significantly impaired in active ulcerative colitis. The addition of compounds interfering with the beta-oxidation pathway had no effect on the butyrate metabolism in UC.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MUCOSAL TISSUE STRIPS, CHAIN FATTY-ACIDS, IN-VIVO, INTESTINAL INFLAMMATION, RECTAL IRRIGATION, CONTROLLED-TRIAL, ENEMAS, COLONOCYTES, DEFICIENCY, LACTATE
journal title
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
Aliment. Pharmacol. Ther.
volume
34
issue
5
pages
526 - 532
Web of Science type
Article
Web of Science id
000293394800004
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
3.769 (2011)
JCR rank
49/259 (2011)
JCR quartile
1 (2011)
ISSN
0269-2813
DOI
10.1111/j.1365-2036.2011.04757.x
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2017061
handle
http://hdl.handle.net/1854/LU-2017061
date created
2012-02-02 19:09:12
date last changed
2016-12-19 15:42:50
@article{2017061,
  abstract     = {Background : Butyrate, a colonic metabolite of carbohydrates, is considered as the major energy source for the colonic mucosa. An impaired butyrate metabolism has been reported in ulcerative colitis (UC), however, the cause still remains unknown. 
Aim : In the present study, we investigated whether higher butyrate concentrations could normalise the oxidation rate in UC. Furthermore, it was investigated whether carnitine could enhance the butyrate oxidation. 
Methods : Mucosal biopsies from a total of 26 UC patients and 25 controls were incubated with (14)C-labelled Na-butyrate and the produced (14)CO(2) was measured. First, the rate of oxidative metabolism was compared at three different concentrations of Na-butyrate (0.05 mM, 1 mM and 10 mM). Then, incubations of biopsies were performed with carnitine alone or combined with ATP. 
Results : Overall, butyrate oxidation in UC was significantly lower than that in controls. The maximum rate of butyrate oxidation was achieved in UC and control subjects from 1 mM onwards. Increasing the butyrate concentration to a level to be present in the colonic lumen, i.e. 10 mM, did not increase the rate of butyrate oxidation in UC to the rate observed in controls. Addition of carnitine alone or combined with ATP caused no effects. 
Conclusions : Saturation of butyrate kinetics was achieved from 1 mM in UC and control subjects. The rate of butyrate metabolism was significantly impaired in active ulcerative colitis. The addition of compounds interfering with the beta-oxidation pathway had no effect on the butyrate metabolism in UC.},
  author       = {De Preter, V and Geboes, Karen and Bulteel, V and Vandermeulen, G and Suenaert, P and Rutgeerts, P and Verbeke, K},
  issn         = {0269-2813},
  journal      = {ALIMENTARY PHARMACOLOGY \& THERAPEUTICS},
  keyword      = {MUCOSAL TISSUE STRIPS,CHAIN FATTY-ACIDS,IN-VIVO,INTESTINAL INFLAMMATION,RECTAL IRRIGATION,CONTROLLED-TRIAL,ENEMAS,COLONOCYTES,DEFICIENCY,LACTATE},
  language     = {eng},
  number       = {5},
  pages        = {526--532},
  title        = {Kinetics of butyrate metabolism in the normal colon and in ulcerative colitis: the effects of substrate concentration and carnitine on the b-oxidation pathway},
  url          = {http://dx.doi.org/10.1111/j.1365-2036.2011.04757.x},
  volume       = {34},
  year         = {2011},
}

Chicago
De Preter, V, Karen Geboes, V Bulteel, G Vandermeulen, P Suenaert, P Rutgeerts, and K Verbeke. 2011. “Kinetics of Butyrate Metabolism in the Normal Colon and in Ulcerative Colitis: The Effects of Substrate Concentration and Carnitine on the B-oxidation Pathway.” Alimentary Pharmacology & Therapeutics 34 (5): 526–532.
APA
De Preter, V., Geboes, K., Bulteel, V., Vandermeulen, G., Suenaert, P., Rutgeerts, P., & Verbeke, K. (2011). Kinetics of butyrate metabolism in the normal colon and in ulcerative colitis: the effects of substrate concentration and carnitine on the b-oxidation pathway. ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 34(5), 526–532.
Vancouver
1.
De Preter V, Geboes K, Bulteel V, Vandermeulen G, Suenaert P, Rutgeerts P, et al. Kinetics of butyrate metabolism in the normal colon and in ulcerative colitis: the effects of substrate concentration and carnitine on the b-oxidation pathway. ALIMENTARY PHARMACOLOGY & THERAPEUTICS. 2011;34(5):526–32.
MLA
De Preter, V, Karen Geboes, V Bulteel, et al. “Kinetics of Butyrate Metabolism in the Normal Colon and in Ulcerative Colitis: The Effects of Substrate Concentration and Carnitine on the B-oxidation Pathway.” ALIMENTARY PHARMACOLOGY & THERAPEUTICS 34.5 (2011): 526–532. Print.