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The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells

LARA DERYCKE UGent, Christophe Stove UGent, Anne-Sophie Vercoutter-Edouart, Olivier De Wever UGent, Laurent Dollé, Nathalie Colpaert UGent, Herman Depypere UGent, Jean-Claude Michalski and Marc Bracke UGent (2011) INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 55(7-9). p.835-840
abstract
Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
aggregation, invasion, MCF-7, breast cancer, MAMMARY-CARCINOMA CELLS, MYH9, non-muscle myosin, MIGRATION, ISOFORMS, ADHESION, INVITRO, RHO, INVADOPODIA, PODOSOMES, INTEGRITY, DEFECTS
journal title
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
Int. J. Dev. Biol.
volume
55
issue
7-9
pages
835 - 840
Web of Science type
Article
Web of Science id
000299655900018
JCR category
DEVELOPMENTAL BIOLOGY
JCR impact factor
2.823 (2011)
JCR rank
19/40 (2011)
JCR quartile
2 (2011)
ISSN
0214-6282
DOI
10.1387/ijdb.1133361d
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2015032
handle
http://hdl.handle.net/1854/LU-2015032
date created
2012-02-01 15:41:10
date last changed
2012-02-24 15:21:56
@article{2015032,
  abstract     = {Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment.},
  author       = {DERYCKE, LARA and Stove, Christophe and Vercoutter-Edouart, Anne-Sophie and De Wever, Olivier and Doll{\'e}, Laurent and Colpaert, Nathalie and Depypere, Herman and Michalski, Jean-Claude and Bracke, Marc},
  issn         = {0214-6282},
  journal      = {INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY},
  keyword      = {aggregation,invasion,MCF-7,breast cancer,MAMMARY-CARCINOMA CELLS,MYH9,non-muscle myosin,MIGRATION,ISOFORMS,ADHESION,INVITRO,RHO,INVADOPODIA,PODOSOMES,INTEGRITY,DEFECTS},
  language     = {eng},
  number       = {7-9},
  pages        = {835--840},
  title        = {The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells},
  url          = {http://dx.doi.org/10.1387/ijdb.1133361d},
  volume       = {55},
  year         = {2011},
}

Chicago
DERYCKE, LARA, Christophe Stove, Anne-Sophie Vercoutter-Edouart, Olivier De Wever, Laurent Dollé, Nathalie Colpaert, Herman Depypere, Jean-Claude Michalski, and Marc Bracke. 2011. “The Role of Non-muscle Myosin IIA in Aggregation and Invasion of Human MCF-7 Breast Cancer Cells.” International Journal of Developmental Biology 55 (7-9): 835–840.
APA
DERYCKE, LARA, Stove, C., Vercoutter-Edouart, A.-S., De Wever, O., Dollé, L., Colpaert, N., Depypere, H., et al. (2011). The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 55(7-9), 835–840.
Vancouver
1.
DERYCKE L, Stove C, Vercoutter-Edouart A-S, De Wever O, Dollé L, Colpaert N, et al. The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2011;55(7-9):835–40.
MLA
DERYCKE, LARA, Christophe Stove, Anne-Sophie Vercoutter-Edouart, et al. “The Role of Non-muscle Myosin IIA in Aggregation and Invasion of Human MCF-7 Breast Cancer Cells.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 55.7-9 (2011): 835–840. Print.