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The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells

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Abstract
Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment.
Keywords
aggregation, invasion, MCF-7, breast cancer, MAMMARY-CARCINOMA CELLS, MYH9, non-muscle myosin, MIGRATION, ISOFORMS, ADHESION, INVITRO, RHO, INVADOPODIA, PODOSOMES, INTEGRITY, DEFECTS

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Chicago
Derycke, Lara, Christophe Stove, Anne-Sophie Vercoutter-Edouart, Olivier De Wever, Laurent Dollé, Nathalie Colpaert, Herman Depypere, Jean-Claude Michalski, and Marc Bracke. 2011. “The Role of Non-muscle Myosin IIA in Aggregation and Invasion of Human MCF-7 Breast Cancer Cells.” International Journal of Developmental Biology 55 (7-9): 835–840.
APA
Derycke, Lara, Stove, C., Vercoutter-Edouart, A.-S., De Wever, O., Dollé, L., Colpaert, N., Depypere, H., et al. (2011). The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 55(7-9), 835–840.
Vancouver
1.
Derycke L, Stove C, Vercoutter-Edouart A-S, De Wever O, Dollé L, Colpaert N, et al. The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2011;55(7-9):835–40.
MLA
Derycke, Lara, Christophe Stove, Anne-Sophie Vercoutter-Edouart, et al. “The Role of Non-muscle Myosin IIA in Aggregation and Invasion of Human MCF-7 Breast Cancer Cells.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 55.7-9 (2011): 835–840. Print.
@article{2015032,
  abstract     = {Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment.},
  author       = {Derycke, Lara and Stove, Christophe and Vercoutter-Edouart, Anne-Sophie and De Wever, Olivier and Doll{\'e}, Laurent and Colpaert, Nathalie and Depypere, Herman and Michalski, Jean-Claude and Bracke, Marc},
  issn         = {0214-6282},
  journal      = {INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY},
  keyword      = {aggregation,invasion,MCF-7,breast cancer,MAMMARY-CARCINOMA CELLS,MYH9,non-muscle myosin,MIGRATION,ISOFORMS,ADHESION,INVITRO,RHO,INVADOPODIA,PODOSOMES,INTEGRITY,DEFECTS},
  language     = {eng},
  number       = {7-9},
  pages        = {835--840},
  title        = {The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells},
  url          = {http://dx.doi.org/10.1387/ijdb.1133361d},
  volume       = {55},
  year         = {2011},
}

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