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Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA

Ivan Ingelbrecht, CI Mandelbaum and TE Mirkov (1998) BIOTECHNIQUES. 25(3). p.420-425
abstract
A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h. The resulting blots give increased sensitivity over existing methods without affecting RNA integrity and can be used in both radioactive and nonradioactive detection procedures.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
BIOTECHNIQUES
Biotechniques
volume
25
issue
3
pages
420 - 425
Web of Science type
Article
Web of Science id
000075865900014
ISSN
0736-6205
language
English
UGent publication?
no
classification
A1
id
2014578
handle
http://hdl.handle.net/1854/LU-2014578
date created
2012-02-01 12:04:28
date last changed
2016-12-19 15:45:06
@article{2014578,
  abstract     = {A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h. The resulting blots give increased sensitivity over existing methods without affecting RNA integrity and can be used in both radioactive and nonradioactive detection procedures.},
  author       = {Ingelbrecht, Ivan and Mandelbaum, CI and Mirkov, TE},
  issn         = {0736-6205},
  journal      = {BIOTECHNIQUES},
  language     = {eng},
  number       = {3},
  pages        = {420--425},
  title        = {Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA},
  volume       = {25},
  year         = {1998},
}

Chicago
Ingelbrecht, Ivan, CI Mandelbaum, and TE Mirkov. 1998. “Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA.” Biotechniques 25 (3): 420–425.
APA
Ingelbrecht, I., Mandelbaum, C., & Mirkov, T. (1998). Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA. BIOTECHNIQUES, 25(3), 420–425.
Vancouver
1.
Ingelbrecht I, Mandelbaum C, Mirkov T. Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA. BIOTECHNIQUES. 1998;25(3):420–5.
MLA
Ingelbrecht, Ivan, CI Mandelbaum, and TE Mirkov. “Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA.” BIOTECHNIQUES 25.3 (1998): 420–425. Print.