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Posttranscriptional gene silencing in transgenic sugarcane: dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome

Ivan Ingelbrecht, James E Irvine and T Erik Mirkov (1999) PLANT PHYSIOLOGY. 119(4). p.1187-1198
abstract
RNA-mediated, posttranscriptional gene silencing has been determined as the molecular mechanism underlying transgenic virus resistance in many plant virus-dicot host plant systems. In this paper we show that transgenic virus resistance in sugarcane (Saccharum spp. hybrid) is based on posttranscriptional gene silencing. The resistance is derived from an untranslatable form of the sorghum mosaic potyvirus strain SCH coat protein (CP) gene. Transgenic sugarcane plants challenged with sorghum mosaic potyvirus strain SCH had phenotypes that ranged from fully susceptible to completely resistant, and a recovery phenotype was also observed. Clones derived from the same transformation event or obtained after vegetative propagation could display different levels of virus resistance, suggesting the involvement of a quantitative component in the resistance response. Most resistant plants displayed low or undetectable steady-state CP transgene mRNA levels, although nuclear transcription rates were high. Increased DNA methylation was observed in the transcribed region of the CP transgenes in most of these plants. Collectively, these characteristics indicate that an RNA-mediated, homology-dependent mechanism is at the base of the virus resistance. This work extends posttranscriptional gene silencing and homology-dependent virus resistance, so far observed only in dicots, to an agronomically important, polyploid monocot.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HIGH-LEVEL EXPRESSION, PATHOGEN-DERIVED RESISTANCE, MOSAIC-VIRUS, PLANTS, RNA, SEQUENCE, SUPPRESSION, INFECTION, SENSE, BOMBARDMENT
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
119
issue
4
pages
1187 - 1198
Web of Science type
Article
Web of Science id
000079737900005
ISSN
0032-0889
DOI
10.1104/pp.119.4.1187
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2014535
handle
http://hdl.handle.net/1854/LU-2014535
date created
2012-02-01 11:53:49
date last changed
2016-12-19 15:45:06
@article{2014535,
  abstract     = {RNA-mediated, posttranscriptional gene silencing has been determined as the molecular mechanism underlying transgenic virus resistance in many plant virus-dicot host plant systems. In this paper we show that transgenic virus resistance in sugarcane (Saccharum spp. hybrid) is based on posttranscriptional gene silencing. The resistance is derived from an untranslatable form of the sorghum mosaic potyvirus strain SCH coat protein (CP) gene. Transgenic sugarcane plants challenged with sorghum mosaic potyvirus strain SCH had phenotypes that ranged from fully susceptible to completely resistant, and a recovery phenotype was also observed. Clones derived from the same transformation event or obtained after vegetative propagation could display different levels of virus resistance, suggesting the involvement of a quantitative component in the resistance response. Most resistant plants displayed low or undetectable steady-state CP transgene mRNA levels, although nuclear transcription rates were high. Increased DNA methylation was observed in the transcribed region of the CP transgenes in most of these plants. Collectively, these characteristics indicate that an RNA-mediated, homology-dependent mechanism is at the base of the virus resistance. This work extends posttranscriptional gene silencing and homology-dependent virus resistance, so far observed only in dicots, to an agronomically important, polyploid monocot.},
  author       = {Ingelbrecht, Ivan and Irvine, James E and Mirkov, T Erik},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {HIGH-LEVEL EXPRESSION,PATHOGEN-DERIVED RESISTANCE,MOSAIC-VIRUS,PLANTS,RNA,SEQUENCE,SUPPRESSION,INFECTION,SENSE,BOMBARDMENT},
  language     = {eng},
  number       = {4},
  pages        = {1187--1198},
  title        = {Posttranscriptional gene silencing in transgenic sugarcane: dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome},
  url          = {http://dx.doi.org/10.1104/pp.119.4.1187},
  volume       = {119},
  year         = {1999},
}

Chicago
Ingelbrecht, Ivan, James E Irvine, and T Erik Mirkov. 1999. “Posttranscriptional Gene Silencing in Transgenic Sugarcane: Dissection of Homology-dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome.” Plant Physiology 119 (4): 1187–1198.
APA
Ingelbrecht, I., Irvine, J. E., & Mirkov, T. E. (1999). Posttranscriptional gene silencing in transgenic sugarcane: dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. PLANT PHYSIOLOGY, 119(4), 1187–1198.
Vancouver
1.
Ingelbrecht I, Irvine JE, Mirkov TE. Posttranscriptional gene silencing in transgenic sugarcane: dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. PLANT PHYSIOLOGY. 1999;119(4):1187–98.
MLA
Ingelbrecht, Ivan, James E Irvine, and T Erik Mirkov. “Posttranscriptional Gene Silencing in Transgenic Sugarcane: Dissection of Homology-dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome.” PLANT PHYSIOLOGY 119.4 (1999): 1187–1198. Print.