Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis
- Author
- Dirk de Graaf (UGent) , Paul Berghen, Hans Hilderson, Hilde De Cock and Jozef Vercruysse (UGent)
- Organization
- Abstract
- Cooperia oncophora total adult extracts were examined by Western blotting with sera from C. oncophora- and O. ostertagi-infected calves to determine species-specific antigens. It was shown that two antigens with apparent molecular weights of 14.2 and 14.9 kDa were only recognized by calves which received a Cooperia infection and not by Ostertagia mono-infected calves or parasite-naive animals. The partial purification of these two antigens was achieved by gel filtration and ion-exchange chromatography. An enzyme-linked immunosorbent assay (ELISA) was developed based on the fractions containing these two antigens and no cross-reactivity could be noticed with serum from Ostertagia mono-infected calves. In contrast, the ELISA with total worm extracts showed strong cross-reactivity with heterologous serum. It was concluded that the 14.2 and 14.9 kDa Cooperia adult antigens have diagnostic potential, at least to differentiate C. oncophora and O. ostertagi.
- Keywords
- CATTLE, COOPERIA-ONCOPHORA, OSTERTAGIA-OSTERTAGI, ELISA, ANTIGEN, SERODIAGNOSIS, OSTERTAGIA, PROTEINS, CALVES
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-201246
- MLA
- de Graaf, Dirk, et al. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 23, no. 1, 1993, pp. 141–44, doi:10.1016/0020-7519(93)90108-B.
- APA
- de Graaf, D., Berghen, P., Hilderson, H., De Cock, H., & Vercruysse, J. (1993). Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 23(1), 141–144. https://doi.org/10.1016/0020-7519(93)90108-B
- Chicago author-date
- Graaf, Dirk de, Paul Berghen, Hans Hilderson, Hilde De Cock, and Jozef Vercruysse. 1993. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY 23 (1): 141–44. https://doi.org/10.1016/0020-7519(93)90108-B.
- Chicago author-date (all authors)
- de Graaf, Dirk, Paul Berghen, Hans Hilderson, Hilde De Cock, and Jozef Vercruysse. 1993. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY 23 (1): 141–144. doi:10.1016/0020-7519(93)90108-B.
- Vancouver
- 1.de Graaf D, Berghen P, Hilderson H, De Cock H, Vercruysse J. Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis. INTERNATIONAL JOURNAL FOR PARASITOLOGY. 1993;23(1):141–4.
- IEEE
- [1]D. de Graaf, P. Berghen, H. Hilderson, H. De Cock, and J. Vercruysse, “Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis,” INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 23, no. 1, pp. 141–144, 1993.
@article{201246,
abstract = {{Cooperia oncophora total adult extracts were examined by Western blotting with sera from C. oncophora- and O. ostertagi-infected calves to determine species-specific antigens. It was shown that two antigens with apparent molecular weights of 14.2 and 14.9 kDa were only recognized by calves which received a Cooperia infection and not by Ostertagia mono-infected calves or parasite-naive animals. The partial purification of these two antigens was achieved by gel filtration and ion-exchange chromatography. An enzyme-linked immunosorbent assay (ELISA) was developed based on the fractions containing these two antigens and no cross-reactivity could be noticed with serum from Ostertagia mono-infected calves. In contrast, the ELISA with total worm extracts showed strong cross-reactivity with heterologous serum. It was concluded that the 14.2 and 14.9 kDa Cooperia adult antigens have diagnostic potential, at least to differentiate C. oncophora and O. ostertagi.}},
author = {{de Graaf, Dirk and Berghen, Paul and Hilderson, Hans and De Cock, Hilde and Vercruysse, Jozef}},
issn = {{0020-7519}},
journal = {{INTERNATIONAL JOURNAL FOR PARASITOLOGY}},
keywords = {{CATTLE,COOPERIA-ONCOPHORA,OSTERTAGIA-OSTERTAGI,ELISA,ANTIGEN,SERODIAGNOSIS,OSTERTAGIA,PROTEINS,CALVES}},
language = {{eng}},
number = {{1}},
pages = {{141--144}},
title = {{Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis}},
url = {{http://dx.doi.org/10.1016/0020-7519(93)90108-B}},
volume = {{23}},
year = {{1993}},
}
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