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Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis

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Abstract
Cooperia oncophora total adult extracts were examined by Western blotting with sera from C. oncophora- and O. ostertagi-infected calves to determine species-specific antigens. It was shown that two antigens with apparent molecular weights of 14.2 and 14.9 kDa were only recognized by calves which received a Cooperia infection and not by Ostertagia mono-infected calves or parasite-naive animals. The partial purification of these two antigens was achieved by gel filtration and ion-exchange chromatography. An enzyme-linked immunosorbent assay (ELISA) was developed based on the fractions containing these two antigens and no cross-reactivity could be noticed with serum from Ostertagia mono-infected calves. In contrast, the ELISA with total worm extracts showed strong cross-reactivity with heterologous serum. It was concluded that the 14.2 and 14.9 kDa Cooperia adult antigens have diagnostic potential, at least to differentiate C. oncophora and O. ostertagi.
Keywords
CATTLE, COOPERIA-ONCOPHORA, OSTERTAGIA-OSTERTAGI, ELISA, ANTIGEN, SERODIAGNOSIS, OSTERTAGIA, PROTEINS, CALVES

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MLA
de Graaf, Dirk, et al. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 23, no. 1, 1993, pp. 141–44, doi:10.1016/0020-7519(93)90108-B.
APA
de Graaf, D., Berghen, P., Hilderson, H., De Cock, H., & Vercruysse, J. (1993). Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 23(1), 141–144. https://doi.org/10.1016/0020-7519(93)90108-B
Chicago author-date
Graaf, Dirk de, Paul Berghen, Hans Hilderson, Hilde De Cock, and Jozef Vercruysse. 1993. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY 23 (1): 141–44. https://doi.org/10.1016/0020-7519(93)90108-B.
Chicago author-date (all authors)
de Graaf, Dirk, Paul Berghen, Hans Hilderson, Hilde De Cock, and Jozef Vercruysse. 1993. “Identification and Purification of Cooperia Oncophora-Specific Antigens to Improve Serological Diagnosis.” INTERNATIONAL JOURNAL FOR PARASITOLOGY 23 (1): 141–144. doi:10.1016/0020-7519(93)90108-B.
Vancouver
1.
de Graaf D, Berghen P, Hilderson H, De Cock H, Vercruysse J. Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis. INTERNATIONAL JOURNAL FOR PARASITOLOGY. 1993;23(1):141–4.
IEEE
[1]
D. de Graaf, P. Berghen, H. Hilderson, H. De Cock, and J. Vercruysse, “Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis,” INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 23, no. 1, pp. 141–144, 1993.
@article{201246,
  abstract     = {{Cooperia oncophora total adult extracts were examined by Western blotting with sera from C. oncophora- and O. ostertagi-infected calves to determine species-specific antigens. It was shown that two antigens with apparent molecular weights of 14.2 and 14.9 kDa were only recognized by calves which received a Cooperia infection and not by Ostertagia mono-infected calves or parasite-naive animals. The partial purification of these two antigens was achieved by gel filtration and ion-exchange chromatography. An enzyme-linked immunosorbent assay (ELISA) was developed based on the fractions containing these two antigens and no cross-reactivity could be noticed with serum from Ostertagia mono-infected calves. In contrast, the ELISA with total worm extracts showed strong cross-reactivity with heterologous serum. It was concluded that the 14.2 and 14.9 kDa Cooperia adult antigens have diagnostic potential, at least to differentiate C. oncophora and O. ostertagi.}},
  author       = {{de Graaf, Dirk and Berghen, Paul and Hilderson, Hans and De Cock, Hilde and Vercruysse, Jozef}},
  issn         = {{0020-7519}},
  journal      = {{INTERNATIONAL JOURNAL FOR PARASITOLOGY}},
  keywords     = {{CATTLE,COOPERIA-ONCOPHORA,OSTERTAGIA-OSTERTAGI,ELISA,ANTIGEN,SERODIAGNOSIS,OSTERTAGIA,PROTEINS,CALVES}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{141--144}},
  title        = {{Identification and purification of Cooperia oncophora-specific antigens to improve serological diagnosis}},
  url          = {{http://dx.doi.org/10.1016/0020-7519(93)90108-B}},
  volume       = {{23}},
  year         = {{1993}},
}

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