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Catalase-negative peroxisomes in human embryonic liver

Marc Espeel (UGent) , N Brière, Dirk De Craemer (UGent) , E Jauniaux and Frank Roels (UGent)
(1993) CELL AND TISSUE RESEARCH. 272(1). p.89-92
Author
Organization
Abstract
Hepatic peroxisomes in human embryos with a menstrual age of 6 and 7 weeks have been examined via catalase cytochemistry. In the younger sample, the organelles show no catalase activity, their matrix being pale and coarsely reticular. In the 7-week specimen, the peroxisome population consists of catalase-positive and catalase-negative organelles. The latter have a morphology identical to that of the 6-week sample and represent 66% of the population. The positive organelles show a pronounced staining hetereogeneity. Together with the simultaneous presence of negative organelles, this might reflect the onset of catalase import into the peroxisomes during this period. Catalase heterogeneity excludes a continuous exchange of matrix contents; moreover, interconnections between peroxisomes have not been observed, and no cluster formation occurs. The data therefore also suggest that catalase is imported into individual, preexisting organelles in embryonic liver. The three peroxisomal beta-oxidation enzymes become detectable by immunocytochemistry only later during development. Morphological indications for a rapidly dividing population, such as elongated and/or tailed organelles, have not been observed. Morphometry has revealed that, in these early stages, the organelles are significantly smaller than the peroxisomes of fetal and adult human liver.
Keywords
ENZYMES, PEROXISOMES, OXIDASE, SIZE, SECTIONS, RAT-LIVER, LIVER, DEVELOPMENT, FETAL, HEPATOCELLULAR PEROXISOMES, CATALASE, BIOGENESIS, MAN

Citation

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Chicago
Espeel, Marc, N Brière, Dirk De Craemer, E Jauniaux, and Frank Roels. 1993. “Catalase-negative Peroxisomes in Human Embryonic Liver.” Cell and Tissue Research 272 (1): 89–92.
APA
Espeel, M., Brière, N., De Craemer, D., Jauniaux, E., & Roels, F. (1993). Catalase-negative peroxisomes in human embryonic liver. CELL AND TISSUE RESEARCH, 272(1), 89–92.
Vancouver
1.
Espeel M, Brière N, De Craemer D, Jauniaux E, Roels F. Catalase-negative peroxisomes in human embryonic liver. CELL AND TISSUE RESEARCH. 1993;272(1):89–92.
MLA
Espeel, Marc, N Brière, Dirk De Craemer, et al. “Catalase-negative Peroxisomes in Human Embryonic Liver.” CELL AND TISSUE RESEARCH 272.1 (1993): 89–92. Print.
@article{201196,
  abstract     = {Hepatic peroxisomes in human embryos with a menstrual age of 6 and 7 weeks have been examined via catalase cytochemistry. In the younger sample, the organelles show no catalase activity, their matrix being pale and coarsely reticular. In the 7-week specimen, the peroxisome population consists of catalase-positive and catalase-negative organelles. The latter have a morphology identical to that of the 6-week sample and represent 66\% of the population. The positive organelles show a pronounced staining hetereogeneity. Together with the simultaneous presence of negative organelles, this might reflect the onset of catalase import into the peroxisomes during this period. Catalase heterogeneity excludes a continuous exchange of matrix contents; moreover, interconnections between peroxisomes have not been observed, and no cluster formation occurs. The data therefore also suggest that catalase is imported into individual, preexisting organelles in embryonic liver. The three peroxisomal beta-oxidation enzymes become detectable by immunocytochemistry only later during development. Morphological indications for a rapidly dividing population, such as elongated and/or tailed organelles, have not been observed. Morphometry has revealed that, in these early stages, the organelles are significantly smaller than the peroxisomes of fetal and adult human liver.},
  author       = {Espeel, Marc and Bri{\`e}re, N and De Craemer, Dirk and Jauniaux, E and Roels, Frank},
  issn         = {0302-766X},
  journal      = {CELL AND TISSUE RESEARCH},
  keyword      = {ENZYMES,PEROXISOMES,OXIDASE,SIZE,SECTIONS,RAT-LIVER,LIVER,DEVELOPMENT,FETAL,HEPATOCELLULAR PEROXISOMES,CATALASE,BIOGENESIS,MAN},
  language     = {eng},
  number       = {1},
  pages        = {89--92},
  title        = {Catalase-negative peroxisomes in human embryonic liver},
  url          = {http://dx.doi.org/10.1007/BF00323574},
  volume       = {272},
  year         = {1993},
}

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