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Improved solvent extraction procedure and HPLC-ELSD method for analysis of polar lipids from dairy materials

Trung Thien Le UGent, Mai Nguyen Tuyet UGent, Jelena Miocinovic UGent, Roeland Rombaut, John Van Camp UGent and Koen Dewettinck UGent (2011) Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts.
abstract
A normal phase HPLC-ELSD method which was improved from the method of Rombaut et al. 2005 (J. Dairy Sci. 88(2):482-488) for analysis of polar lipids (PLs) is presented. In the improved method, the mobile phase consisted of two lines; dichloromethane and a mixture of methanol and acetic acid/triethylamine buffer. Dichloromethane is less toxic than chloroform which was used in the old method. PLs of interest such as glycolipids, phospholipids and sphingomyelin were well separated with a total time for one analysis run of 22.5 min. Peak retention times and peak area were reproducible due to a good miscibility of mobile phases. The improved HPLC-ELSD method was applicable for both PLs from soy lecithin and dairy materials. Furthermore, a modified solvent extraction method of PLs from dairy matrices was adapted. The modified method offered higher extraction efficiency, consumed less time and in some cases saved solvent use.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
phospholipids, Polar lipids, HPLC, ELSD, MFGM, extraction
in
Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts
conference name
2nd Conference on Food Science and Technology : Mekong Delta, Vietnam (MekongFood 2) : Food safety and food quality in Souteast Asia : challenges for the next decade
conference location
Can Tho, Vietnam
conference start
2011-11-09
conference end
2011-11-12
language
English
UGent publication?
yes
classification
C3
additional info
uploaded document is poster version
copyright statement
I have retained and own the full copyright for this publication
id
2009821
handle
http://hdl.handle.net/1854/LU-2009821
date created
2012-01-31 14:52:54
date last changed
2012-02-07 10:51:14
@inproceedings{2009821,
  abstract     = {A normal phase HPLC-ELSD method which was improved from the method of Rombaut et al. 2005 (J. Dairy Sci. 88(2):482-488) for analysis of polar lipids (PLs) is presented. In the improved method, the mobile phase consisted of two lines; dichloromethane and a mixture of methanol and acetic acid/triethylamine buffer. Dichloromethane is less toxic than chloroform which was used in the old method. PLs of interest such as glycolipids, phospholipids and sphingomyelin were well separated with a total time for one analysis run of 22.5 min. Peak retention times and peak area were reproducible due to a good miscibility of mobile phases. The improved HPLC-ELSD method was applicable for both PLs from soy lecithin and dairy materials. Furthermore, a modified solvent extraction method of PLs from dairy matrices was adapted. The modified method offered higher extraction efficiency, consumed less time and in some cases saved solvent use.},
  author       = {Le, Trung Thien and Nguyen Tuyet, Mai and Miocinovic, Jelena and Rombaut, Roeland and Van Camp, John and Dewettinck, Koen},
  booktitle    = {Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts},
  keyword      = {phospholipids,Polar lipids,HPLC,ELSD,MFGM,extraction},
  language     = {eng},
  location     = {Can Tho, Vietnam},
  title        = {Improved solvent extraction procedure and HPLC-ELSD method for analysis of polar lipids from dairy materials},
  year         = {2011},
}

Chicago
Le, Trung Thien, Mai Nguyen Tuyet, Jelena Miocinovic, Roeland Rombaut, John Van Camp, and Koen Dewettinck. 2011. “Improved Solvent Extraction Procedure and HPLC-ELSD Method for Analysis of Polar Lipids from Dairy Materials.” In Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts.
APA
Le, T. T., Nguyen Tuyet, M., Miocinovic, J., Rombaut, R., Van Camp, J., & Dewettinck, K. (2011). Improved solvent extraction procedure and HPLC-ELSD method for analysis of polar lipids from dairy materials. Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts. Presented at the 2nd Conference on Food Science and Technology : Mekong Delta, Vietnam (MekongFood 2) : Food safety and food quality in Souteast Asia : challenges for the next decade.
Vancouver
1.
Le TT, Nguyen Tuyet M, Miocinovic J, Rombaut R, Van Camp J, Dewettinck K. Improved solvent extraction procedure and HPLC-ELSD method for analysis of polar lipids from dairy materials. Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts. 2011.
MLA
Le, Trung Thien, Mai Nguyen Tuyet, Jelena Miocinovic, et al. “Improved Solvent Extraction Procedure and HPLC-ELSD Method for Analysis of Polar Lipids from Dairy Materials.” Food Science and Technology : Mekong Delta, Vietnam, 2nd Conference, Abstracts. 2011. Print.