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Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes

Hanne H Rasmussen, Jozef Van Damme UGent, Magda Puype UGent, Borbala Gesser, Julio E Celis and Joël Vandekerckhove (1992) ELECTROPHORESIS. 13(12). p.960-969
abstract
Microsequencing of proteins recovered from two-dimensional (2-D) gels is being used systematically to identify proteins in the master human keratinocyte 2-D gel database. To date, about 250 protein spots recorded in human 2-D gel databases have been microsequenced and, of these, 145 are recorded in the keratinocyte database under the entry partial amino acid sequence. Coomassie Brilliant Blue-stained protein spots cut from several (up to 40) dry gels were concentrated by elution-concentration gel electrophoresis, electroblotted onto PVDF membranes and digested in situ with trypsin. Eluting peptides were separated by reversed-phase HPLC, collected individually and sequenced. Computer search using the FASTA and TFASTA programs from Genetics Computer Group indicated that 110 of the microsequenced polypeptides shared significant similarity with proteins contained in the PIR, Mipsx or GenEMBL databases. Only 35 polypeptides corresponded to hitherto unknown proteins. Peptide sequences of all 145 proteins are listed together with their coordinates (apparent molecular weight and pI) in the keratinocyte database.
Please use this url to cite or link to this publication:
author
organization
alternative title
Microsequences of 145 proteins recorded in the 2-dimensional gel protein database of normal human epidermal keratinocytes
year
type
journalArticle (original)
publication status
published
subject
keyword
CELLS, LINK, DNA-SEQUENCE DATA
journal title
ELECTROPHORESIS
Electrophoresis
volume
13
issue
12
pages
960 - 969
Web of Science type
Article
ISSN
0173-0835
DOI
10.1002/elps.11501301199
language
English
UGent publication?
yes
classification
A1
id
200797
handle
http://hdl.handle.net/1854/LU-200797
date created
2004-01-14 13:42:00
date last changed
2016-12-19 15:40:34
@article{200797,
  abstract     = {Microsequencing of proteins recovered from two-dimensional (2-D) gels is being used systematically to identify proteins in the master human keratinocyte 2-D gel database. To date, about 250 protein spots recorded in human 2-D gel databases have been microsequenced and, of these, 145 are recorded in the keratinocyte database under the entry partial amino acid sequence. Coomassie Brilliant Blue-stained protein spots cut from several (up to 40) dry gels were concentrated by elution-concentration gel electrophoresis, electroblotted onto PVDF membranes and digested in situ with trypsin. Eluting peptides were separated by reversed-phase HPLC, collected individually and sequenced. Computer search using the FASTA and TFASTA programs from Genetics Computer Group indicated that 110 of the microsequenced polypeptides shared significant similarity with proteins contained in the PIR, Mipsx or GenEMBL databases. Only 35 polypeptides corresponded to hitherto unknown proteins. Peptide sequences of all 145 proteins are listed together with their coordinates (apparent molecular weight and pI) in the keratinocyte database.},
  author       = {Rasmussen, Hanne H and Van Damme, Jozef and Puype, Magda and Gesser, Borbala and Celis, Julio E and Vandekerckhove, Jo{\"e}l},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {CELLS,LINK,DNA-SEQUENCE DATA},
  language     = {eng},
  number       = {12},
  pages        = {960--969},
  title        = {Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes},
  url          = {http://dx.doi.org/10.1002/elps.11501301199},
  volume       = {13},
  year         = {1992},
}

Chicago
Rasmussen, Hanne H, Jozef Van Damme, Magda Puype, Borbala Gesser, Julio E Celis, and Joël Vandekerckhove. 1992. “Microsequences of 145 Proteins Recorded in the Two-dimensional Gel Protein Database of Normal Human Epidermal Keratinocytes.” Electrophoresis 13 (12): 960–969.
APA
Rasmussen, H. H., Van Damme, J., Puype, M., Gesser, B., Celis, J. E., & Vandekerckhove, J. (1992). Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes. ELECTROPHORESIS, 13(12), 960–969.
Vancouver
1.
Rasmussen HH, Van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J. Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes. ELECTROPHORESIS. 1992;13(12):960–9.
MLA
Rasmussen, Hanne H, Jozef Van Damme, Magda Puype, et al. “Microsequences of 145 Proteins Recorded in the Two-dimensional Gel Protein Database of Normal Human Epidermal Keratinocytes.” ELECTROPHORESIS 13.12 (1992): 960–969. Print.