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T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs

Stéphane De Craeye UGent, Malgorzata Jennes UGent, Delfien Verhelst UGent, Pierre Dorny UGent, Katelijne Dierick, Vesna Melkebeek UGent and Eric Cox UGent (2011) Toxoplasmosis, 11th International congress, Abstracts.
abstract
One of the major routes of a Toxoplasma gondii infection in humans is the consumption of raw or undercooked meat. In the present study, we compared the parasitic load induced by 2 different T. gondii strains in the tissues of experimentally infected 6 weeks old pigs. In the first experiment, pigs were orally infected with 3000 tissue cysts of IPB-Gangji strain. The pigs were euthanized 2 and 6 months after infection, and the following samples were tested by bio-assay and qPCR: brain, heart and several skeletal muscles. Two months after infection, all samples tested positive with both tests. Remarkably, after 6 month no cysts were detected in tenderloin and ham, while brain and heart tissue remained infectious. In the second experiment, pigs were infected orally with a low (700 cysts) and a high (6000) dose of T. gondii IPB-Gangji cysts and euthanized after 4 months. The parasitic load was much higher in the low dose group than in the high dose group, as determined by qPCR. In most animals various samples tested negative in both groups, with the exception of the intercostals muscles. Last experiment was repeated with a low and a high dose of the T. gondii IPB-LR strain. Here, all samples remained infectious with no significant difference in parasitic load between both groups. The parasitic load was higher in brain and heart tissue compared to the skeletal muscles. In bio-assay, numerous mice died from the inoculated samples from pigs infected with the IPB-Gangji strain. Ascites and lungs tested T. gondii positive by qPCR. When inoculated with samples from pigs infected with the IPB-LR strain, no mice died from acute T. gondii infection.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs
in
Toxoplasmosis, 11th International congress, Abstracts
conference name
11th International congress on Toxoplasmosis (TOXO-11)
conference location
Ottawa, ON, Canada
conference start
2011-06-25
conference end
2011-06-29
language
English
UGent publication?
yes
classification
C3
copyright statement
I have transferred the copyright for this publication to the publisher
id
2007862
handle
http://hdl.handle.net/1854/LU-2007862
date created
2012-01-30 15:16:47
date last changed
2012-03-22 11:02:28
@inproceedings{2007862,
  abstract     = {One of the major routes of a Toxoplasma gondii infection in humans is the consumption of raw or undercooked meat. In the present study, we compared the parasitic load induced by 2 different T. gondii strains in the tissues of experimentally infected 6 weeks old pigs.
In the first experiment, pigs were orally infected with 3000 tissue cysts of IPB-Gangji strain. The pigs were euthanized 2 and 6 months after infection, and the following samples were tested by bio-assay and qPCR: brain, heart and several skeletal muscles. Two months after infection, all samples tested positive with both tests. Remarkably, after 6 month no cysts were detected in tenderloin and ham, while brain and heart tissue remained infectious.
In the second experiment, pigs were infected orally with a low (700 cysts) and a high (6000) dose of T. gondii IPB-Gangji cysts and euthanized after 4 months. The parasitic load was much higher in the low dose group than in the high dose group, as determined by qPCR. In most animals various samples tested negative in both groups, with the exception of the intercostals muscles.
Last experiment was repeated with a low and a high dose of the T. gondii IPB-LR strain. Here, all samples remained infectious with no significant difference in parasitic load between both groups. The parasitic load was higher in brain and heart tissue compared to the skeletal muscles.
In bio-assay, numerous mice died from the inoculated samples from pigs infected with the IPB-Gangji strain. Ascites and lungs tested T. gondii positive by qPCR. When inoculated with samples from pigs infected with the IPB-LR strain, no mice died from acute T. gondii infection.},
  author       = {De Craeye, St{\'e}phane and Jennes, Malgorzata and Verhelst, Delfien and Dorny, Pierre and Dierick, Katelijne and Melkebeek, Vesna and Cox, Eric},
  booktitle    = {Toxoplasmosis, 11th International congress, Abstracts},
  keyword      = {T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs},
  language     = {eng},
  location     = {Ottawa, ON, Canada},
  title        = {T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs},
  year         = {2011},
}

Chicago
De Craeye, Stéphane, Malgorzata Jennes, Delfien Verhelst, Pierre Dorny, Katelijne Dierick, Vesna Melkebeek, and Eric Cox. 2011. “T. Gondii Strains and Their Dosage Influence the Parasitic Load in Tissues of Experimentally Infected Pigs.” In Toxoplasmosis, 11th International Congress, Abstracts.
APA
De Craeye, S., Jennes, M., Verhelst, D., Dorny, P., Dierick, K., Melkebeek, V., & Cox, E. (2011). T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs. Toxoplasmosis, 11th International congress, Abstracts. Presented at the 11th International congress on Toxoplasmosis (TOXO-11).
Vancouver
1.
De Craeye S, Jennes M, Verhelst D, Dorny P, Dierick K, Melkebeek V, et al. T. gondii strains and their dosage influence the parasitic load in tissues of experimentally infected pigs. Toxoplasmosis, 11th International congress, Abstracts. 2011.
MLA
De Craeye, Stéphane, Malgorzata Jennes, Delfien Verhelst, et al. “T. Gondii Strains and Their Dosage Influence the Parasitic Load in Tissues of Experimentally Infected Pigs.” Toxoplasmosis, 11th International Congress, Abstracts. 2011. Print.