Advanced search
1 file | 94.88 KB

Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding

Maryam Atef Yekta (UGent) , Bruno Goddeeris (UGent) , Daisy Vanrompay (UGent) and Eric Cox (UGent)
Author
Organization
Abstract
Enterohaemorrhagic Escherichia coli O157:H7 are zoonotic pathogens associated with haemorrhagic colitis (HC) and the haemolytic uremic syndrome (HUS). Ruminants are the main reservoir of this organism and most outbreaks of E. coli O157:H7 infections are food borne. Food contamination by ruminant manure has been reported as the primary source of human infection, therefore inhibition of E. coli O157:H7 colonization and shedding in ruminants could control the risk of human exposure to this pathogen. In the present study a vaccine based on the translocon proteins EspA and EspB and the outer membrane adhesion factor intiminγ significantly reduced faecal shedding of E. coli O157:H7 by orally infected sheep. Protection correlates with serum antibody responses to the defined antigens and validates the targeting of these colonization factors. Preliminary studies on the mechanism of this immune response shows presence of antigen-specific IgG antibody secreting cells in the mucosae of the small intestine. Whereas vaccination has already been described in cattle, this is the first study describing a significant decrease in faecal shedding following systemic immunization of sheep.

Downloads

  • Immunization of sheep with a combination of intiming.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 94.88 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Atef Yekta, Maryam, Bruno Goddeeris, Daisy Vanrompay, and Eric Cox. 2011. “Immunization of Sheep with a Combination of Intiminγ, EspA and EspB Decreases Escherichia Coli O157:H7 Shedding.” In Vaccines and Adjuvants for Emerging Infectious Diseases, Abstracts.
APA
Atef Yekta, M., Goddeeris, B., Vanrompay, D., & Cox, E. (2011). Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding. Vaccines and Adjuvants for Emerging Infectious Diseases, Abstracts. Presented at the Vaccines and Adjuvants for Emerging Infectious Diseases (VAEID 2011).
Vancouver
1.
Atef Yekta M, Goddeeris B, Vanrompay D, Cox E. Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding. Vaccines and Adjuvants for Emerging Infectious Diseases, Abstracts. 2011.
MLA
Atef Yekta, Maryam, Bruno Goddeeris, Daisy Vanrompay, et al. “Immunization of Sheep with a Combination of Intiminγ, EspA and EspB Decreases Escherichia Coli O157:H7 Shedding.” Vaccines and Adjuvants for Emerging Infectious Diseases, Abstracts. 2011. Print.
@inproceedings{2007602,
  abstract     = {Enterohaemorrhagic Escherichia coli O157:H7 are zoonotic pathogens associated with haemorrhagic colitis (HC) and the haemolytic uremic syndrome (HUS). Ruminants are the main reservoir of this organism and most outbreaks of E. coli O157:H7 infections are food borne. Food contamination by ruminant manure has been reported as the primary source of human infection, therefore inhibition of E. coli O157:H7 colonization and shedding in ruminants could control the risk of human exposure to this pathogen. In the present study a vaccine based on the translocon proteins EspA and EspB and the outer membrane adhesion factor intiminγ significantly reduced faecal shedding of E. coli O157:H7 by orally infected sheep. Protection correlates with serum antibody responses to the defined antigens and validates the targeting of these colonization factors. Preliminary studies on the mechanism of this immune response shows presence of antigen-specific IgG antibody secreting cells in the mucosae of the small intestine. Whereas vaccination has already been described in cattle, this is the first study describing a significant decrease in faecal shedding following systemic immunization of sheep.},
  author       = {Atef Yekta, Maryam and Goddeeris, Bruno and Vanrompay, Daisy and Cox, Eric},
  booktitle    = {Vaccines and Adjuvants for Emerging Infectious Diseases, Abstracts},
  language     = {eng},
  location     = {Montego Bay, Jamaica},
  title        = {Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding},
  year         = {2011},
}