Ghent University Academic Bibliography

Advanced

(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women

P Švec, I Sedláček, M Chrápavá and Peter Vandamme UGent (2011) FOLIA MICROBIOLOGICA. 56(1). p.80-83
abstract
A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)(5)-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)(5)-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L. jensenii strains were distributed over two fingerprint clusters. The majority of samples was dominated by one (GTG)(5)-PCR type. The rep-PCR fingerprinting using the (GTG)(5) primer allowed straightforward identification of many, but not all, isolates. This method has been shown to be a useful tool for fast screening and grouping of vaginal lactobacilli, but its combination with another identification method is needed to obtain reliable identification results. In addition, Lactobacillus acidophilus was not shown to be the most common inhabitant of the female genital tract as generally assumed.
Please use this url to cite or link to this publication:
author
organization
alternative title
(GTG)(5)-PCR fingerprinting of lactobacilli isolated from cervix of healthy women
year
type
journalArticle (original)
publication status
published
subject
keyword
MICROBIOTA, IDENTIFICATION, LACTIC-ACID BACTERIA, DIVERSITY
journal title
FOLIA MICROBIOLOGICA
Folia Microbiol.
volume
56
issue
1
pages
80 - 83
Web of Science type
Article
Web of Science id
000290451500014
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
0.677 (2011)
JCR rank
136/157 (2011)
JCR quartile
4 (2011)
ISSN
0015-5632
DOI
10.1007/s12223-011-0006-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2006481
handle
http://hdl.handle.net/1854/LU-2006481
date created
2012-01-27 13:21:06
date last changed
2016-12-19 15:45:06
@article{2006481,
  abstract     = {A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)(5)-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)(5)-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L. jensenii strains were distributed over two fingerprint clusters. The majority of samples was dominated by one (GTG)(5)-PCR type. The rep-PCR fingerprinting using the (GTG)(5) primer allowed straightforward identification of many, but not all, isolates. This method has been shown to be a useful tool for fast screening and grouping of vaginal lactobacilli, but its combination with another identification method is needed to obtain reliable identification results. In addition, Lactobacillus acidophilus was not shown to be the most common inhabitant of the female genital tract as generally assumed.},
  author       = {\v{S}vec, P and Sedl{\'a}\v{c}ek, I and Chr{\'a}pav{\'a}, M and Vandamme, Peter},
  issn         = {0015-5632},
  journal      = {FOLIA MICROBIOLOGICA},
  keyword      = {MICROBIOTA,IDENTIFICATION,LACTIC-ACID BACTERIA,DIVERSITY},
  language     = {eng},
  number       = {1},
  pages        = {80--83},
  title        = {(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women},
  url          = {http://dx.doi.org/10.1007/s12223-011-0006-4},
  volume       = {56},
  year         = {2011},
}

Chicago
Švec, P, I Sedláček, M Chrápavá, and Peter Vandamme. 2011. “(GTG)5-PCR Fingerprinting of Lactobacilli Isolated from Cervix of Healthy Women.” Folia Microbiologica 56 (1): 80–83.
APA
Švec, P, Sedláček, I., Chrápavá, M., & Vandamme, P. (2011). (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women. FOLIA MICROBIOLOGICA, 56(1), 80–83.
Vancouver
1.
Švec P, Sedláček I, Chrápavá M, Vandamme P. (GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women. FOLIA MICROBIOLOGICA. 2011;56(1):80–3.
MLA
Švec, P, I Sedláček, M Chrápavá, et al. “(GTG)5-PCR Fingerprinting of Lactobacilli Isolated from Cervix of Healthy Women.” FOLIA MICROBIOLOGICA 56.1 (2011): 80–83. Print.