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Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania

Taranjit Kaur, Jatinder Singh, Michael A Huffman, Klára J Petrželková, Nancy S Taylor, ShiLu Xu, Floyd E Dewhirst, Bruce J Paster, Lies Debruyne UGent, Peter Vandamme UGent, et al. (2011) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 77(7). p.2366-2373
abstract
The transmission of simian immunodeficiency and Ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. In this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (Pan troglodytes) populations with different histories in relation to their proximity to humans. Campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research and tourism purposes at Mahale Mountains National Park) and 0/15 (0%) group 2 (not human habituated but propagated from an introduced population released from captivity over 30 years ago at Rubondo Island National Park) chimpanzees, respectively. Using 16S rRNA gene sequencing, all isolates were virtually identical (at most a single base difference), and the chimpanzee isolates were most closely related to Campylobacter helveticus and Campylobacter upsaliensis (94.7% and 95.9% similarity, respectively). Whole-cell protein profiling, amplified fragment length polymorphism analysis of genomic DNA, hsp60 sequence analysis, and determination of the mol% G+C content revealed two subgroups among the chimpanzee isolates. DNA-DNA hybridization experiments confirmed that both subgroups represented distinct genomic species. In the absence of differential biochemical characteristics and morphology and identical 16S rRNA gene sequences, we propose to classify all isolates into a single novel nomenspecies, Campylobacter troglodytis, with strain MIT 05-9149 as the type strain; strain MIT 05-9157 is suggested as the reference strain for the second C. troglodytis genomovar. Further studies are required to determine whether the organism is pathogenic to chimpanzees and whether this novel Campylobacter colonizes humans and causes enteric disease.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MAHALE MOUNTAINS, INTESTINAL PARASITES, GENETIC RELATEDNESS, MACACA-NEMESTRINA, NATIONAL-PARK, GREAT APES, IDENTIFICATION, JEJUNI, HELICOBACTER, UPSALIENSIS
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
77
issue
7
pages
2366 - 2373
Web of Science type
Article
Web of Science id
000288855500022
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.829 (2011)
JCR rank
29/157 (2011)
JCR quartile
1 (2011)
ISSN
0099-2240
DOI
10.1128/AEM.01840-09
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2006468
handle
http://hdl.handle.net/1854/LU-2006468
date created
2012-01-27 13:21:06
date last changed
2016-12-19 15:42:54
@article{2006468,
  abstract     = {The transmission of simian immunodeficiency and Ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. In this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (Pan troglodytes) populations with different histories in relation to their proximity to humans. Campylobacter spp. were detected by culture in 19/56 (34\%) group 1 (human habituated for research and tourism purposes at Mahale Mountains National Park) and 0/15 (0\%) group 2 (not human habituated but propagated from an introduced population released from captivity over 30 years ago at Rubondo Island National Park) chimpanzees, respectively. Using 16S rRNA gene sequencing, all isolates were virtually identical (at most a single base difference), and the chimpanzee isolates were most closely related to Campylobacter helveticus and Campylobacter upsaliensis (94.7\% and 95.9\% similarity, respectively). Whole-cell protein profiling, amplified fragment length polymorphism analysis of genomic DNA, hsp60 sequence analysis, and determination of the mol\% G+C content revealed two subgroups among the chimpanzee isolates. DNA-DNA hybridization experiments confirmed that both subgroups represented distinct genomic species. In the absence of differential biochemical characteristics and morphology and identical 16S rRNA gene sequences, we propose to classify all isolates into a single novel nomenspecies, Campylobacter troglodytis, with strain MIT 05-9149 as the type strain; strain MIT 05-9157 is suggested as the reference strain for the second C. troglodytis genomovar. Further studies are required to determine whether the organism is pathogenic to chimpanzees and whether this novel Campylobacter colonizes humans and causes enteric disease.},
  author       = {Kaur, Taranjit and Singh, Jatinder and Huffman, Michael A and Petr\v{z}elkov{\'a}, Kl{\'a}ra J and Taylor, Nancy S and Xu, ShiLu and Dewhirst, Floyd E and Paster, Bruce J and Debruyne, Lies and Vandamme, Peter and Fox, James G},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {MAHALE MOUNTAINS,INTESTINAL PARASITES,GENETIC RELATEDNESS,MACACA-NEMESTRINA,NATIONAL-PARK,GREAT APES,IDENTIFICATION,JEJUNI,HELICOBACTER,UPSALIENSIS},
  language     = {eng},
  number       = {7},
  pages        = {2366--2373},
  title        = {Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania},
  url          = {http://dx.doi.org/10.1128/AEM.01840-09},
  volume       = {77},
  year         = {2011},
}

Chicago
Kaur, Taranjit, Jatinder Singh, Michael A Huffman, Klára J Petrželková, Nancy S Taylor, ShiLu Xu, Floyd E Dewhirst, et al. 2011. “Campylobacter Troglodytis Sp. Nov., Isolated from Feces of Human-habituated Wild Chimpanzees (Pan Troglodytes Schweinfurthii) in Tanzania.” Applied and Environmental Microbiology 77 (7): 2366–2373.
APA
Kaur, T., Singh, J., Huffman, M. A., Petrželková, K. J., Taylor, N. S., Xu, S., Dewhirst, F. E., et al. (2011). Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(7), 2366–2373.
Vancouver
1.
Kaur T, Singh J, Huffman MA, Petrželková KJ, Taylor NS, Xu S, et al. Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2011;77(7):2366–73.
MLA
Kaur, Taranjit, Jatinder Singh, Michael A Huffman, et al. “Campylobacter Troglodytis Sp. Nov., Isolated from Feces of Human-habituated Wild Chimpanzees (Pan Troglodytes Schweinfurthii) in Tanzania.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.7 (2011): 2366–2373. Print.