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Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community

Ebrahim Hossein Najdegerami UGent, Ngoc Tran Tiet UGent, Tom Defoirdt UGent, Massimo Marzorati UGent, Patrick Sorgeloos UGent, Nico Boon UGent and Peter Bossier UGent (2012) FEMS MICROBIOLOGY ECOLOGY. 79(1). p.25-33
abstract
Poly-beta-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog(TM) Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects.
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author
organization
alternative title
Effects of poly-ss-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community
year
type
journalArticle (original)
publication status
published
subject
keyword
DIETARY SUPPLEMENTATION, SUBSTRATE UTILIZATION, GROWTH-PERFORMANCE, X MORONE-SAXATILIS, MANNAN OLIGOSACCHARIDES MOS, PATHOGENIC VIBRIO-CAMPBELLII, GRADIENT GEL-ELECTROPHORESIS, microbial community, Siberian sturgeon, prebiotics, poly-β-hydroxybutyrate, ARTEMIA-FRANCISCANA, BREWERS-YEAST, PREBIOTICS
journal title
FEMS MICROBIOLOGY ECOLOGY
FEMS Microbiol. Ecol.
volume
79
issue
1
pages
25 - 33
Web of Science type
Article
Web of Science id
000298302600004
JCR category
MICROBIOLOGY
JCR impact factor
3.563 (2012)
JCR rank
31/116 (2012)
JCR quartile
2 (2012)
ISSN
0168-6496
DOI
10.1111/j.1574-6941.2011.01194.x
language
English
UGent publication?
yes
classification
A1
additional info
alternative title: as in Web of Science
copyright statement
I have transferred the copyright for this publication to the publisher
id
2005246
handle
http://hdl.handle.net/1854/LU-2005246
date created
2012-01-26 16:39:31
date last changed
2013-12-20 08:29:21
@article{2005246,
  abstract     = {Poly-beta-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2\% and 5\% PHB were investigated. Replacing 2\% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2\% PHB. DNA sequencing of the dominant bands in 2\% and 5\% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog(TM) Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2\% of Siberian sturgeon fingerling diet with PHB has beneficial effects.},
  author       = {Hossein Najdegerami, Ebrahim and Tiet, Ngoc Tran and Defoirdt, Tom and Marzorati, Massimo and Sorgeloos, Patrick and Boon, Nico and Bossier, Peter},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {DIETARY SUPPLEMENTATION,SUBSTRATE UTILIZATION,GROWTH-PERFORMANCE,X MORONE-SAXATILIS,MANNAN OLIGOSACCHARIDES MOS,PATHOGENIC VIBRIO-CAMPBELLII,GRADIENT GEL-ELECTROPHORESIS,microbial community,Siberian sturgeon,prebiotics,poly-\ensuremath{\beta}-hydroxybutyrate,ARTEMIA-FRANCISCANA,BREWERS-YEAST,PREBIOTICS},
  language     = {eng},
  number       = {1},
  pages        = {25--33},
  title        = {Effects of poly-\ensuremath{\beta}-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2011.01194.x},
  volume       = {79},
  year         = {2012},
}

Chicago
Hossein Najdegerami, Ebrahim, Ngoc Tran Tiet, Tom Defoirdt, Massimo Marzorati, Patrick Sorgeloos, Nico Boon, and Peter Bossier. 2012. “Effects of Poly-β-hydroxybutyrate (PHB) on Siberian Sturgeon (Acipenser Baerii) Fingerlings Performance and Its Gastrointestinal Tract Microbial Community.” Fems Microbiology Ecology 79 (1): 25–33.
APA
Hossein Najdegerami, E., Tiet, N. T., Defoirdt, T., Marzorati, M., Sorgeloos, P., Boon, N., & Bossier, P. (2012). Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community. FEMS MICROBIOLOGY ECOLOGY, 79(1), 25–33.
Vancouver
1.
Hossein Najdegerami E, Tiet NT, Defoirdt T, Marzorati M, Sorgeloos P, Boon N, et al. Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community. FEMS MICROBIOLOGY ECOLOGY. 2012;79(1):25–33.
MLA
Hossein Najdegerami, Ebrahim, Ngoc Tran Tiet, Tom Defoirdt, et al. “Effects of Poly-β-hydroxybutyrate (PHB) on Siberian Sturgeon (Acipenser Baerii) Fingerlings Performance and Its Gastrointestinal Tract Microbial Community.” FEMS MICROBIOLOGY ECOLOGY 79.1 (2012): 25–33. Print.