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Partial resolution of molecular species during liquid chromatography of soybean phospholipids and effect on quantitation by light scattering

(1992) CHROMATOGRAPHIA. 34(11-12). p.557-562
Author
Organization
Abstract
Using both synthetic phospholipids and soybean phosphatidylcholine (PC), it is shown that the retention of phospholipids during normal-phase chromatography is not only determined by the polar headgroup, but also by the fatty acid composition. Although fatty acid methyl ester determinations reveal that this effect only causes an enrichment of some fatty acids in subsequent fractions of the same phospholipid class, still a large influence on the peak shape is observed. As a further consequence, the evaporative light scattering detector output is greatly affected, so that accurate quantitation of phospholipid classes requires the availability of standards of the same origin.
Keywords
COLUMN LIQUID CHROMATOGRAPHY, SOYBEAN LECITHIN, PARTIAL RESOLUTION, LIGHT SCATTERING DETECTION, MASS DETECTOR, SEPARATION, GLYCOSPHINGOLIPIDS, OPTIMIZATION, LIPIDS, ESTERS, HPLC

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Chicago
Van Der Meeren, Paul, Jean Vanderdeelen, G Huyghebaert, and Leon Baert. 1992. “Partial Resolution of Molecular Species During Liquid Chromatography of Soybean Phospholipids and Effect on Quantitation by Light Scattering.” Chromatographia 34 (11-12): 557–562.
APA
Van Der Meeren, P., Vanderdeelen, J., Huyghebaert, G., & Baert, L. (1992). Partial resolution of molecular species during liquid chromatography of soybean phospholipids and effect on quantitation by light scattering. CHROMATOGRAPHIA, 34(11-12), 557–562.
Vancouver
1.
Van Der Meeren P, Vanderdeelen J, Huyghebaert G, Baert L. Partial resolution of molecular species during liquid chromatography of soybean phospholipids and effect on quantitation by light scattering. CHROMATOGRAPHIA. 1992;34(11-12):557–62.
MLA
Van Der Meeren, Paul, Jean Vanderdeelen, G Huyghebaert, et al. “Partial Resolution of Molecular Species During Liquid Chromatography of Soybean Phospholipids and Effect on Quantitation by Light Scattering.” CHROMATOGRAPHIA 34.11-12 (1992): 557–562. Print.
@article{200428,
  abstract     = {Using both synthetic phospholipids and soybean phosphatidylcholine (PC), it is shown that the retention of phospholipids during normal-phase chromatography is not only determined by the polar headgroup, but also by the fatty acid composition. Although fatty acid methyl ester determinations reveal that this effect only causes an enrichment of some fatty acids in subsequent fractions of the same phospholipid class, still a large influence on the peak shape is observed. As a further consequence, the evaporative light scattering detector output is greatly affected, so that accurate quantitation of phospholipid classes requires the availability of standards of the same origin.},
  author       = {Van Der Meeren, Paul and Vanderdeelen, Jean and Huyghebaert, G and Baert, Leon},
  issn         = {0009-5893},
  journal      = {CHROMATOGRAPHIA},
  keyword      = {COLUMN LIQUID CHROMATOGRAPHY,SOYBEAN LECITHIN,PARTIAL RESOLUTION,LIGHT SCATTERING DETECTION,MASS DETECTOR,SEPARATION,GLYCOSPHINGOLIPIDS,OPTIMIZATION,LIPIDS,ESTERS,HPLC},
  language     = {eng},
  number       = {11-12},
  pages        = {557--562},
  title        = {Partial resolution of molecular species during liquid chromatography of soybean phospholipids and effect on quantitation by light scattering},
  volume       = {34},
  year         = {1992},
}