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Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury

Thomas Volckaert UGent, Erik Dill, Alice Campbell, Caterina Tiozzo, Susan Majka, Saverio Bellusci and Stijn P De Langhe (2011) JOURNAL OF CLINICAL INVESTIGATION. 121(11). p.4409-4419
abstract
During lung development, parabronchial SMC (PSMC) progenitors in the distal mesenchyme secrete fibroblast growth factor 10 (Fgf10), which acts on distal epithelial progenitors to promote their proliferation. beta-catenin signaling within PSMC progenitors is essential for their maintenance, proliferation, and expression of Fgf10. Here, we report that this Wnt/Fgf10 embryonic signaling cascade is reactivated in mature PSMCs after naphthalene-induced injury to airway epithelium. Furthermore, we found that this paracrine Fgf10 action was essential for activating surviving variant Clara cells (the cells in the airway epithelium from which replacement epithelial cells originate) located at the bronchoalveolar duct junctions and adjacent to neuroendocrine bodies. After naphthalene injury, PSMCs secreted Fgf10 to activate Notch signaling and induce Snail expression in surviving variant Clara cells, which subsequently underwent a transient epithelial to mesenchymal transition to initiate the repair process. Epithelial Snail expression was important for regeneration after injury. We have therefore identified PSMCs as a stem cell niche for the variant Clara cells in the lung and established that paracrine Fgf10 signaling from the niche is critical for epithelial repair after naphthalene injury. These findings also have implications for understanding the misregulation of lung repair in asthma and cancer.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROGENITOR CELLS, BRANCHING MORPHOGENESIS, FGF10 EXPRESSION, MULTIPLE MESENCHYMAL LINEAGES, MAMMARY-GLAND, TRANSITIONS, DIFFERENTIATION, REGENERATION, DISEASE, MAINTENANCE
journal title
JOURNAL OF CLINICAL INVESTIGATION
J. Clin. Invest.
volume
121
issue
11
pages
4409 - 4419
Web of Science type
Article
Web of Science id
000296482700023
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
13.069 (2011)
JCR rank
4/109 (2011)
JCR quartile
1 (2011)
ISSN
0021-9738
DOI
10.1172/JCI58097
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2003590
handle
http://hdl.handle.net/1854/LU-2003590
date created
2012-01-25 17:46:25
date last changed
2012-06-26 14:32:33
@article{2003590,
  abstract     = {During lung development, parabronchial SMC (PSMC) progenitors in the distal mesenchyme secrete fibroblast growth factor 10 (Fgf10), which acts on distal epithelial progenitors to promote their proliferation. beta-catenin signaling within PSMC progenitors is essential for their maintenance, proliferation, and expression of Fgf10. Here, we report that this Wnt/Fgf10 embryonic signaling cascade is reactivated in mature PSMCs after naphthalene-induced injury to airway epithelium. Furthermore, we found that this paracrine Fgf10 action was essential for activating surviving variant Clara cells (the cells in the airway epithelium from which replacement epithelial cells originate) located at the bronchoalveolar duct junctions and adjacent to neuroendocrine bodies. After naphthalene injury, PSMCs secreted Fgf10 to activate Notch signaling and induce Snail expression in surviving variant Clara cells, which subsequently underwent a transient epithelial to mesenchymal transition to initiate the repair process. Epithelial Snail expression was important for regeneration after injury. We have therefore identified PSMCs as a stem cell niche for the variant Clara cells in the lung and established that paracrine Fgf10 signaling from the niche is critical for epithelial repair after naphthalene injury. These findings also have implications for understanding the misregulation of lung repair in asthma and cancer.},
  author       = {Volckaert, Thomas and Dill, Erik and Campbell, Alice and Tiozzo, Caterina and Majka, Susan and Bellusci, Saverio and De Langhe, Stijn P},
  issn         = {0021-9738},
  journal      = {JOURNAL OF CLINICAL INVESTIGATION},
  keyword      = {PROGENITOR CELLS,BRANCHING MORPHOGENESIS,FGF10 EXPRESSION,MULTIPLE MESENCHYMAL LINEAGES,MAMMARY-GLAND,TRANSITIONS,DIFFERENTIATION,REGENERATION,DISEASE,MAINTENANCE},
  language     = {eng},
  number       = {11},
  pages        = {4409--4419},
  title        = {Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury},
  url          = {http://dx.doi.org/10.1172/JCI58097},
  volume       = {121},
  year         = {2011},
}

Chicago
Volckaert, Thomas, Erik Dill, Alice Campbell, Caterina Tiozzo, Susan Majka, Saverio Bellusci, and Stijn P De Langhe. 2011. “Parabronchial Smooth Muscle Constitutes an Airway Epithelial Stem Cell Niche in the Mouse Lung After Injury.” Journal of Clinical Investigation 121 (11): 4409–4419.
APA
Volckaert, T., Dill, E., Campbell, A., Tiozzo, C., Majka, S., Bellusci, S., & De Langhe, S. P. (2011). Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. JOURNAL OF CLINICAL INVESTIGATION, 121(11), 4409–4419.
Vancouver
1.
Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, et al. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. JOURNAL OF CLINICAL INVESTIGATION. 2011;121(11):4409–19.
MLA
Volckaert, Thomas, Erik Dill, Alice Campbell, et al. “Parabronchial Smooth Muscle Constitutes an Airway Epithelial Stem Cell Niche in the Mouse Lung After Injury.” JOURNAL OF CLINICAL INVESTIGATION 121.11 (2011): 4409–4419. Print.