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VEGF mediates commissural axon chemoattraction through its receptor Flk1

(2011) NEURON. 70(5). p.966-978
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Abstract
Growing axons are guided to their targets by attractive and repulsive cues. In the developing spinal cord, Netrin-1 and Shh guide commissural axons toward the midline. However, the combined inhibition of their activity in commissural axon turning assays does not completely abrogate turning toward floor plate tissue, suggesting that additional guidance cues are present. Here we show that the prototypic angiogenic factor VEGF is secreted by the floor plate and is a chemoattractant for commissural axons in vitro and in vivo. Inactivation of Vegf in the floor plate or of its receptor Flk1 in commissural neurons causes axon guidance defects, whereas Flk1 blockade inhibits turning of axons to VEGF in vitro. Similar to Shh and Netrin-1, VEGF-mediated commissural axon guidance requires the activity of Src family kinases. Our results identify VEGF and Flk1 as a novel ligand/receptor pair controlling connmissural axon guidance.
Keywords
NERVOUS-SYSTEM, FOCAL ADHESION KINASE, ENDOTHELIAL GROWTH-FACTOR, SONIC HEDGEHOG, NEURAL-TUBE, SIGNALING PATHWAY, FACIAL-NERVE, IN-VIVO, GUIDANCE, EXPRESSION

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Chicago
Ruiz de Almodovar, Carmen, Pierre J Fabre, Ellen Knevels, Cathy Coulon, Inmaculada Segura, Patrick CG Haddick, Liesbeth Aerts, et al. 2011. “VEGF Mediates Commissural Axon Chemoattraction Through Its Receptor Flk1.” Neuron 70 (5): 966–978.
APA
Ruiz de Almodovar, C., Fabre, P. J., Knevels, E., Coulon, C., Segura, I., Haddick, P. C., Aerts, L., et al. (2011). VEGF mediates commissural axon chemoattraction through its receptor Flk1. NEURON, 70(5), 966–978.
Vancouver
1.
Ruiz de Almodovar C, Fabre PJ, Knevels E, Coulon C, Segura I, Haddick PC, et al. VEGF mediates commissural axon chemoattraction through its receptor Flk1. NEURON. 2011;70(5):966–78.
MLA
Ruiz de Almodovar, Carmen, Pierre J Fabre, Ellen Knevels, et al. “VEGF Mediates Commissural Axon Chemoattraction Through Its Receptor Flk1.” NEURON 70.5 (2011): 966–978. Print.
@article{2003495,
  abstract     = {Growing axons are guided to their targets by attractive and repulsive cues. In the developing spinal cord, Netrin-1 and Shh guide commissural axons toward the midline. However, the combined inhibition of their activity in commissural axon turning assays does not completely abrogate turning toward floor plate tissue, suggesting that additional guidance cues are present. Here we show that the prototypic angiogenic factor VEGF is secreted by the floor plate and is a chemoattractant for commissural axons in vitro and in vivo. Inactivation of Vegf in the floor plate or of its receptor Flk1 in commissural neurons causes axon guidance defects, whereas Flk1 blockade inhibits turning of axons to VEGF in vitro. Similar to Shh and Netrin-1, VEGF-mediated commissural axon guidance requires the activity of Src family kinases. Our results identify VEGF and Flk1 as a novel ligand/receptor pair controlling connmissural axon guidance.},
  author       = {Ruiz de Almodovar, Carmen and Fabre, Pierre J and Knevels, Ellen and Coulon, Cathy and Segura, Inmaculada and Haddick, Patrick CG and Aerts, Liesbeth and Delattin, Nicolas and Strasser, Geraldine and Oh, Won-Jong and Lange, Christian and Vinckier, Stefan and Haigh, Jody and Fouquet, Coralie and Gu, Chengua and Alitalo, Kari and Castellani, Valerie and Tessier-Lavigne, Marc and Chedotal, Alain and Charron, Frederic and Carmeliet, Peter},
  issn         = {0896-6273},
  journal      = {NEURON},
  keyword      = {NERVOUS-SYSTEM,FOCAL ADHESION KINASE,ENDOTHELIAL GROWTH-FACTOR,SONIC HEDGEHOG,NEURAL-TUBE,SIGNALING PATHWAY,FACIAL-NERVE,IN-VIVO,GUIDANCE,EXPRESSION},
  language     = {eng},
  number       = {5},
  pages        = {966--978},
  title        = {VEGF mediates commissural axon chemoattraction through its receptor Flk1},
  url          = {http://dx.doi.org/10.1016/j.neuron.2011.04.014},
  volume       = {70},
  year         = {2011},
}

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