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Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent

Sharen Provoost UGent, Tania Maes UGent, Guy Joos UGent and Kurt Tournoy UGent (2012) JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 129(2). p.483-491
abstract
Background: The inhalation of diesel exhaust particles (DEPs) is associated with increased sensitization toward inhaled allergens. Dendritic cells (DCs) are important mediators in immune regulation. We previously showed that the inhalation of DEPs increased the accumulation of DCs in the lung and enhanced the T(H)2 response in the mediastinal lymph node. Objective: We hypothesized that CC chemokine receptors CCR2, CCR5, and CCR6 critically mediate the DC recruitment upon exposure to DEPs and that these CC chemokine receptors are important in the DEP-induced TH2 response. Methods: We exposed CCR2 knockout, CCR5 knockout, CCR6 knockout, and wild-type mice to DEPs and examined the pulmonary monocyte and DC accumulation. By an adoptive transfer experiment, we assessed the direct involvement of CCR2 and CCR6 in the recruitment of blood monocytes toward the lung upon exposure to DEPs. We also examined the TH2 cytokine production in the mediastinal lymph nodes of DEP-exposed CCR2 knockout and CCR6 knockout mice. Results: We observed that the DEP-induced monocyte and monocyte-derived DC recruitment was completely abolished in CCR2 knockout mice. CCR6 knockout mice also showed impaired monocyte recruitment upon exposure to DEPs. In contrast, monocyte and DC recruitment was comparable between DEP-exposed wild-type and CCR5 knockout mice. The impaired monocyte-derived DC recruitment in DEP-exposed CCR2 knockout, not CCR6 knockout, mice resulted in an abolished TH2 response in the mediastinal lymph node. Conclusion: These data suggest that monocyte-derived DCs, recruited in a CCR2-dependent manner, are critical in inducing TH2 responses upon inhalation of DEPs.
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author
organization
alternative title
Monocyte-derived dendritic cell recruitment and allergic T(H)2 responses after exposure to diesel particles are CCR2 dependent
year
type
journalArticle (original)
publication status
published
subject
keyword
CCR5, CCR2, CCR6, dendritic cell, diesel exhaust particles, monocyte, INDUCED PULMONARY INFLAMMATION, CHEMOKINE RECEPTOR CCR2, AIRWAY INFLAMMATION, EXHAUST PARTICLES, EPITHELIAL-CELLS, INHALED ANTIGEN, BONE-MARROW, IN-VIVO, MICE, SUBSETS
journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
J. Allergy Clin. Immunol.
volume
129
issue
2
pages
483 - 491
Web of Science type
Article
Web of Science id
000299951700028
JCR category
ALLERGY
JCR impact factor
12.047 (2012)
JCR rank
1/23 (2012)
JCR quartile
1 (2012)
ISSN
0091-6749
DOI
10.1016/j.jaci.2011.07.051
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2002313
handle
http://hdl.handle.net/1854/LU-2002313
date created
2012-01-25 08:11:44
date last changed
2012-08-03 11:40:15
@article{2002313,
  abstract     = {Background: The inhalation of diesel exhaust particles (DEPs) is associated with increased sensitization toward inhaled allergens. Dendritic cells (DCs) are important mediators in immune regulation. We previously showed that the inhalation of DEPs increased the accumulation of DCs in the lung and enhanced the T(H)2 response in the mediastinal lymph node.
Objective: We hypothesized that CC chemokine receptors CCR2, CCR5, and CCR6 critically mediate the DC recruitment upon exposure to DEPs and that these CC chemokine receptors are important in the DEP-induced TH2 response.
Methods: We exposed CCR2 knockout, CCR5 knockout, CCR6 knockout, and wild-type mice to DEPs and examined the pulmonary monocyte and DC accumulation. By an adoptive transfer experiment, we assessed the direct involvement of CCR2 and CCR6 in the recruitment of blood monocytes toward the lung upon exposure to DEPs. We also examined the TH2 cytokine production in the mediastinal lymph nodes of DEP-exposed CCR2 knockout and CCR6 knockout mice.
Results: We observed that the DEP-induced monocyte and monocyte-derived DC recruitment was completely abolished in CCR2 knockout mice. CCR6 knockout mice also showed impaired monocyte recruitment upon exposure to DEPs. In contrast, monocyte and DC recruitment was comparable between DEP-exposed wild-type and CCR5 knockout mice. The impaired monocyte-derived DC recruitment in DEP-exposed CCR2 knockout, not CCR6 knockout, mice resulted in an abolished TH2 response in the mediastinal lymph node.
Conclusion: These data suggest that monocyte-derived DCs, recruited in a CCR2-dependent manner, are critical in inducing TH2 responses upon inhalation of DEPs.},
  author       = {Provoost, Sharen and Maes, Tania and Joos, Guy and Tournoy, Kurt},
  issn         = {0091-6749},
  journal      = {JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY},
  keyword      = {CCR5,CCR2,CCR6,dendritic cell,diesel exhaust particles,monocyte,INDUCED PULMONARY INFLAMMATION,CHEMOKINE RECEPTOR CCR2,AIRWAY INFLAMMATION,EXHAUST PARTICLES,EPITHELIAL-CELLS,INHALED ANTIGEN,BONE-MARROW,IN-VIVO,MICE,SUBSETS},
  language     = {eng},
  number       = {2},
  pages        = {483--491},
  title        = {Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent},
  url          = {http://dx.doi.org/10.1016/j.jaci.2011.07.051},
  volume       = {129},
  year         = {2012},
}

Chicago
Provoost, Sharen, Tania Maes, Guy Joos, and Kurt Tournoy. 2012. “Monocyte-derived Dendritic Cell Recruitment and Allergic TH2 Responses After Exposure to Diesel Particles Are CCR2 Dependent.” Journal of Allergy and Clinical Immunology 129 (2): 483–491.
APA
Provoost, Sharen, Maes, T., Joos, G., & Tournoy, K. (2012). Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 129(2), 483–491.
Vancouver
1.
Provoost S, Maes T, Joos G, Tournoy K. Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2012;129(2):483–91.
MLA
Provoost, Sharen, Tania Maes, Guy Joos, et al. “Monocyte-derived Dendritic Cell Recruitment and Allergic TH2 Responses After Exposure to Diesel Particles Are CCR2 Dependent.” JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 129.2 (2012): 483–491. Print.