Ghent University Academic Bibliography

Advanced

Life without a cell membrane: challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta)

Joke Hollants UGent, Helen Decleyre UGent, Frédérik Leliaert UGent, Olivier De Clerck UGent and Anne Willems UGent (2011) BMC MICROBIOLOGY. 11.
abstract
Background : The siphonous green macroalga Bryopsis has some remarkable characteristics. Besides hosting a rich endophytic bacterial flora, Bryopsis also displays extraordinary wound repair and propagation mechanisms. This latter feature includes the formation of protoplasts which can survive in the absence of a cell membrane for several minutes before regenerating into new individuals. This transient 'life without a membrane' state, however, challenges the specificity of the endophytic bacterial communities present and raises the question whether these bacteria are generalists, which are repeatedly acquired from the environment, or if there is some specificity towards the Bryopsis host. Results : To answer this question, we examined the temporal stability and the uniqueness of endobiotic bacterial communities within Bryopsis samples from the Mexican west coast after prolonged cultivation. DGGE analysis revealed that Bryopsis endophytic bacterial communities are rather stable and clearly distinct from the epiphytic and surrounding cultivation water bacterial communities. Although these endogenous communities consist of both facultative and obligate bacteria, results suggest that Bryopsis owns some intrinsic mechanisms to selectively maintain and/or attract specific bacteria after repeated wounding events in culture. Conclusions : This suggests that Bryopsis algae seem to master transient stages of life without a cell membrane well as they harbor specific - and possibly ecological significant - endophytic bacteria.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHYLOGENETIC ANALYSIS, DISINTEGRATED CELLS, PROTOPLAST FORMATION, PLUMOSA CHLOROPHYTA, MARINE MACROALGAE, GREEN-ALGAE, REGENERATION, COMMUNITY, LECTIN, BRYOHEALIN
journal title
BMC MICROBIOLOGY
BMC Microbiol.
volume
11
article_number
255
pages
10 pages
Web of Science type
Article
Web of Science id
000298860800001
JCR category
MICROBIOLOGY
JCR impact factor
3.044 (2011)
JCR rank
43/111 (2011)
JCR quartile
2 (2011)
ISSN
1471-2180
DOI
10.1186/1471-2180-11-255
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2001747
handle
http://hdl.handle.net/1854/LU-2001747
date created
2012-01-24 14:41:41
date last changed
2012-02-23 17:24:45
@article{2001747,
  abstract     = {Background : The siphonous green macroalga Bryopsis has some remarkable characteristics. Besides hosting a rich endophytic bacterial flora, Bryopsis also displays extraordinary wound repair and propagation mechanisms. This latter feature includes the formation of protoplasts which can survive in the absence of a cell membrane for several minutes before regenerating into new individuals. This transient 'life without a membrane' state, however, challenges the specificity of the endophytic bacterial communities present and raises the question whether these bacteria are generalists, which are repeatedly acquired from the environment, or if there is some specificity towards the Bryopsis host.
Results : To answer this question, we examined the temporal stability and the uniqueness of endobiotic bacterial communities within Bryopsis samples from the Mexican west coast after prolonged cultivation. DGGE analysis revealed that Bryopsis endophytic bacterial communities are rather stable and clearly distinct from the epiphytic and surrounding cultivation water bacterial communities. Although these endogenous communities consist of both facultative and obligate bacteria, results suggest that Bryopsis owns some intrinsic mechanisms to selectively maintain and/or attract specific bacteria after repeated wounding events in culture.
Conclusions : This suggests that Bryopsis algae seem to master transient stages of life without a cell membrane well as they harbor specific - and possibly ecological significant - endophytic bacteria.},
  articleno    = {255},
  author       = {Hollants, Joke and Decleyre, Helen and Leliaert, Fr{\'e}d{\'e}rik and De Clerck, Olivier and Willems, Anne},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {PHYLOGENETIC ANALYSIS,DISINTEGRATED CELLS,PROTOPLAST FORMATION,PLUMOSA CHLOROPHYTA,MARINE MACROALGAE,GREEN-ALGAE,REGENERATION,COMMUNITY,LECTIN,BRYOHEALIN},
  language     = {eng},
  pages        = {10},
  title        = {Life without a cell membrane: challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta)},
  url          = {http://dx.doi.org/10.1186/1471-2180-11-255},
  volume       = {11},
  year         = {2011},
}

Chicago
Hollants, Joke, Helen Decleyre, Frédérik Leliaert, Olivier De Clerck, and Anne Willems. 2011. “Life Without a Cell Membrane: Challenging the Specificity of Bacterial Endophytes Within Bryopsis (Bryopsidales, Chlorophyta).” Bmc Microbiology 11.
APA
Hollants, J., Decleyre, H., Leliaert, F., De Clerck, O., & Willems, A. (2011). Life without a cell membrane: challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta). BMC MICROBIOLOGY, 11.
Vancouver
1.
Hollants J, Decleyre H, Leliaert F, De Clerck O, Willems A. Life without a cell membrane: challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta). BMC MICROBIOLOGY. 2011;11.
MLA
Hollants, Joke, Helen Decleyre, Frédérik Leliaert, et al. “Life Without a Cell Membrane: Challenging the Specificity of Bacterial Endophytes Within Bryopsis (Bryopsidales, Chlorophyta).” BMC MICROBIOLOGY 11 (2011): n. pag. Print.