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A common system controls the induction of very different genes: the class-A β-lactamase of Proteus vulgaris and the enterobacterial class-C β-lactamase

Martina Datz, Bernard Joris, Essam AM Azab, Moreno Galleni, Jozef Van Beeumen (UGent) , Jean-Marie Frère and Hans H Martin
(1994) EUROPEAN JOURNAL OF BIOCHEMISTRY. 226(1). p.149-157
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Abstract
Among the Enterobacteriaceae, Proteus vulgaris is exceptional in the inducible production of a 29-kDa beta-lactamase (cefuroximase) with an unusually high activity towards the beta-lactamase-stable oximino-cephalosporins (e.g. cefuroxime and cefotaxime). Sequencing of the corresponding gene, cumA, showed that the derived CumA beta-lactamase belonged to the molecular class A. The structural gene was under the direct control of gene cumR, which was transcribed backwards and whose initiation codon was 165 bp away from that of the beta-lactamase gene. This resembled the arrangement of structural and regulator genes ampC and ampR of the 39-kDa molecular-class-C beta-lactamase AmpC present in many enterobacteria. Moreover, cloned genes ampD and ampG for negative modulation and signal transduction of AmpC beta-lactamase induction, respectively, were also able to restore constitutively CumA overproducing and non-inducible P. vulgaris mutants to the inducible, wildtype phenotype. The results indicate that controls of the induction phenomena are equivalent for the CumA and AmpC beta-lactamase. Very different structural genes can thus be under the control of identical systems.
Keywords
AMINO-ACID-SEQUENCE, CITROBACTER-FREUNDII AMPR, ESCHERICHIA-COLI, CEPHALOSPORINASE PRODUCTION, ENZYMATIC AMPLIFICATION, EVOLUTIONARY ORIGIN, NUCLEOTIDE-SEQUENCE, KLEBSIELLA-OXYTOCA, KINETIC-PARAMETERS, CLONING VECTORS

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MLA
Datz, Martina, Bernard Joris, Essam AM Azab, et al. “A Common System Controls the Induction of Very Different Genes: The class-A Β-lactamase of Proteus Vulgaris and the Enterobacterial class-C Β-lactamase.” EUROPEAN JOURNAL OF BIOCHEMISTRY 226.1 (1994): 149–157. Print.
APA
Datz, M., Joris, B., Azab, E. A., Galleni, M., Van Beeumen, J., Frère, J.-M., & Martin, H. H. (1994). A common system controls the induction of very different genes: the class-A β-lactamase of Proteus vulgaris and the enterobacterial class-C β-lactamase. EUROPEAN JOURNAL OF BIOCHEMISTRY, 226(1), 149–157.
Chicago author-date
Datz, Martina, Bernard Joris, Essam AM Azab, Moreno Galleni, Jozef Van Beeumen, Jean-Marie Frère, and Hans H Martin. 1994. “A Common System Controls the Induction of Very Different Genes: The class-A Β-lactamase of Proteus Vulgaris and the Enterobacterial class-C Β-lactamase.” European Journal of Biochemistry 226 (1): 149–157.
Chicago author-date (all authors)
Datz, Martina, Bernard Joris, Essam AM Azab, Moreno Galleni, Jozef Van Beeumen, Jean-Marie Frère, and Hans H Martin. 1994. “A Common System Controls the Induction of Very Different Genes: The class-A Β-lactamase of Proteus Vulgaris and the Enterobacterial class-C Β-lactamase.” European Journal of Biochemistry 226 (1): 149–157.
Vancouver
1.
Datz M, Joris B, Azab EA, Galleni M, Van Beeumen J, Frère J-M, et al. A common system controls the induction of very different genes: the class-A β-lactamase of Proteus vulgaris and the enterobacterial class-C β-lactamase. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1994;226(1):149–57.
IEEE
[1]
M. Datz et al., “A common system controls the induction of very different genes: the class-A β-lactamase of Proteus vulgaris and the enterobacterial class-C β-lactamase,” EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 226, no. 1, pp. 149–157, 1994.
@article{200104,
  abstract     = {Among the Enterobacteriaceae, Proteus vulgaris is exceptional in the inducible production of a 29-kDa beta-lactamase (cefuroximase) with an unusually high activity towards the beta-lactamase-stable oximino-cephalosporins (e.g. cefuroxime and cefotaxime). Sequencing of the corresponding gene, cumA, showed that the derived CumA beta-lactamase belonged to the molecular class A. The structural gene was under the direct control of gene cumR, which was transcribed backwards and whose initiation codon was 165 bp away from that of the beta-lactamase gene. This resembled the arrangement of structural and regulator genes ampC and ampR of the 39-kDa molecular-class-C beta-lactamase AmpC present in many enterobacteria. Moreover, cloned genes ampD and ampG for negative modulation and signal transduction of AmpC beta-lactamase induction, respectively, were also able to restore constitutively CumA overproducing and non-inducible P. vulgaris mutants to the inducible, wildtype phenotype. The results indicate that controls of the induction phenomena are equivalent for the CumA and AmpC beta-lactamase. Very different structural genes can thus be under the control of identical systems.},
  author       = {Datz, Martina and Joris, Bernard and Azab, Essam AM and Galleni, Moreno and Van Beeumen, Jozef and Frère, Jean-Marie and Martin, Hans H},
  issn         = {0014-2956},
  journal      = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
  keywords     = {AMINO-ACID-SEQUENCE,CITROBACTER-FREUNDII AMPR,ESCHERICHIA-COLI,CEPHALOSPORINASE PRODUCTION,ENZYMATIC AMPLIFICATION,EVOLUTIONARY ORIGIN,NUCLEOTIDE-SEQUENCE,KLEBSIELLA-OXYTOCA,KINETIC-PARAMETERS,CLONING VECTORS},
  language     = {eng},
  number       = {1},
  pages        = {149--157},
  title        = {A common system controls the induction of very different genes: the class-A β-lactamase of Proteus vulgaris and the enterobacterial class-C β-lactamase},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.1994.0t149.x},
  volume       = {226},
  year         = {1994},
}
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