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Multilevel analysis of nuclear dynamics in lamin perturbed fibroblasts

Winnok De Vos UGent, Frederik Houben, Jos Broers, Ron A Hoebe, Erik Manders UGent, Greg Joss, Frans Ramaekers and Patric Van Oostveldt UGent (2010) ImageJ, Proceedings. p.130-135
abstract
The nuclear lamina provides structural support to the nucleus and has a central role in defining nuclear organization. Defects in its filamentous constituents, the lamins, lead to a class of diseases collectively referred to as laminopathies. On the cellular level, lamin mutations affect the physical integrity of nuclei and nucleo-cytoskeletal interactions, resulting in increased susceptibility to mechanical stress and altered gene expression [1]. Most studies regarding the mechanical properties of the nucleus in laminopathic conditions are based on the induction of extracellular stress, such as strain or compression, and focus on nuclear integrity and/or nucleo-cytoskeletal interaction [2]. Far less is known about the role of nuclear organization and mobility under basal steady-state conditions. In this study, we quantitatively compared nuclear organization, nuclear deformation and chromatin mobility of fibroblasts from a Hutchinson-Gilford progeria patient with cells from a lamin A/C-deficient patient and wild-type dermal fibroblasts. To this end, we created a toolbox in imageJ for automatically analyzing both nuclear as well as subnuclear dynamics in living cells. Simultaneously, we developed a workflow for comparing cellular morphology and subcellular protein distribution in a high content fashion. We found that the absence of functional lamin A/C leads to increased nuclear plasticity on the hour and minute time scale but also to increased intranuclear mobility down to the seconds time scale. In contrast, progeria cells showed overall reduced nuclear dynamics. In addition, high content analysis revealed marked morphological and topological differences between different culture passages within a cell type and between different pathological variants of culture-age matched laminopathic cell types.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
high content analysis, nuclear shape, tracking, nuclear mobility
in
ImageJ, Proceedings
pages
130 - 135
conference name
ImageJ User and Developer Conference
conference location
Luxembourg, GD Luxembourg
conference start
2010-10-27
conference end
2010-10-29
ISBN
9782919941117
language
English
UGent publication?
yes
classification
C1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2000107
handle
http://hdl.handle.net/1854/LU-2000107
date created
2012-01-23 14:54:23
date last changed
2012-02-21 08:48:14
@inproceedings{2000107,
  abstract     = {The nuclear lamina provides structural support to the nucleus and has a central role in defining nuclear organization. Defects in its filamentous constituents, the lamins, lead to a class of diseases collectively referred to as laminopathies. On the cellular level, lamin mutations affect the physical integrity of nuclei and nucleo-cytoskeletal interactions, resulting in increased susceptibility to mechanical stress and altered gene expression [1]. Most studies regarding the mechanical properties of the nucleus in laminopathic conditions are based on the induction of extracellular stress, such as strain or compression, and focus on nuclear integrity and/or nucleo-cytoskeletal interaction [2]. Far less is known about the role of nuclear organization and mobility under basal steady-state conditions.
In this study, we quantitatively compared nuclear organization, nuclear deformation and chromatin mobility of fibroblasts from a Hutchinson-Gilford progeria patient with cells from a lamin A/C-deficient patient and wild-type dermal fibroblasts. To this end, we created a toolbox in imageJ for automatically analyzing both nuclear as well as subnuclear dynamics in living cells. Simultaneously, we developed a workflow for comparing cellular morphology and subcellular protein distribution in a high content fashion.
We found that the absence of functional lamin A/C leads to increased nuclear plasticity on the hour and minute time scale but also to increased intranuclear mobility down to the seconds time scale. In contrast, progeria cells showed overall reduced nuclear dynamics. In addition, high content analysis revealed marked morphological and topological differences between different culture passages within a cell type and between different pathological variants of culture-age matched laminopathic cell types.},
  author       = {De Vos, Winnok and Houben, Frederik and Broers, Jos  and Hoebe, Ron A and Manders, Erik and Joss, Greg and Ramaekers, Frans and Van Oostveldt, Patric},
  booktitle    = {ImageJ, Proceedings},
  isbn         = {9782919941117},
  keyword      = {high content analysis,nuclear shape,tracking,nuclear mobility},
  language     = {eng},
  location     = {Luxembourg, GD Luxembourg},
  pages        = {130--135},
  title        = {Multilevel analysis of nuclear dynamics in lamin perturbed fibroblasts},
  year         = {2010},
}

Chicago
De Vos, Winnok, Frederik Houben, Jos Broers, Ron A Hoebe, Erik Manders, Greg Joss, Frans Ramaekers, and Patric Van Oostveldt. 2010. “Multilevel Analysis of Nuclear Dynamics in Lamin Perturbed Fibroblasts.” In ImageJ, Proceedings, 130–135.
APA
De Vos, Winnok, Houben, F., Broers, J., Hoebe, R. A., Manders, E., Joss, G., Ramaekers, F., et al. (2010). Multilevel analysis of nuclear dynamics in lamin perturbed fibroblasts. ImageJ, Proceedings (pp. 130–135). Presented at the ImageJ User and Developer Conference.
Vancouver
1.
De Vos W, Houben F, Broers J, Hoebe RA, Manders E, Joss G, et al. Multilevel analysis of nuclear dynamics in lamin perturbed fibroblasts. ImageJ, Proceedings. 2010. p. 130–5.
MLA
De Vos, Winnok, Frederik Houben, Jos Broers, et al. “Multilevel Analysis of Nuclear Dynamics in Lamin Perturbed Fibroblasts.” ImageJ, Proceedings. 2010. 130–135. Print.