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Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation

Ivan Ingelbrecht (UGent) , Helena Van Houdt (UGent) , Marc Van Montagu (UGent) and Anna Depicker (UGent)
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Abstract
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features-i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical moths CG and CXG.
Keywords
SYNTHASE GENE, PLANTS, CO-SUPPRESSION, EUKARYOTIC DNA, INACTIVATION, ASCOBOLUS-IMMERSUS, TRANSPOSABLE ELEMENT, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, CPG BINDING-PROTEIN, NICOTIANA TABACUM, TRANSGENIC PLANT, EPIGENETICS, COSUPPRESSION, CYTOSINE METHYLATION

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Citation

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MLA
Ingelbrecht, Ivan, Helena Van Houdt, Marc Van Montagu, et al. “Posttranscriptional Silencing of Reporter Transgenes in Tobacco Correlates with DNA Methylation.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 91.22 (1994): 10502–10506. Print.
APA
Ingelbrecht, I., Van Houdt, H., Van Montagu, M., & Depicker, A. (1994). Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91(22), 10502–10506.
Chicago author-date
Ingelbrecht, Ivan, Helena Van Houdt, Marc Van Montagu, and Anna Depicker. 1994. “Posttranscriptional Silencing of Reporter Transgenes in Tobacco Correlates with DNA Methylation.” Proceedings of the National Academy of Sciences of the United States of America 91 (22): 10502–10506.
Chicago author-date (all authors)
Ingelbrecht, Ivan, Helena Van Houdt, Marc Van Montagu, and Anna Depicker. 1994. “Posttranscriptional Silencing of Reporter Transgenes in Tobacco Correlates with DNA Methylation.” Proceedings of the National Academy of Sciences of the United States of America 91 (22): 10502–10506.
Vancouver
1.
Ingelbrecht I, Van Houdt H, Van Montagu M, Depicker A. Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1994;91(22):10502–6.
IEEE
[1]
I. Ingelbrecht, H. Van Houdt, M. Van Montagu, and A. Depicker, “Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 91, no. 22, pp. 10502–10506, 1994.
@article{199885,
  abstract     = {Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features-i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical moths CG and CXG.},
  author       = {Ingelbrecht, Ivan and Van Houdt, Helena and Van Montagu, Marc and Depicker, Anna},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {SYNTHASE GENE,PLANTS,CO-SUPPRESSION,EUKARYOTIC DNA,INACTIVATION,ASCOBOLUS-IMMERSUS,TRANSPOSABLE ELEMENT,ARABIDOPSIS-THALIANA,GENE-EXPRESSION,CPG BINDING-PROTEIN,NICOTIANA TABACUM,TRANSGENIC PLANT,EPIGENETICS,COSUPPRESSION,CYTOSINE METHYLATION},
  language     = {eng},
  number       = {22},
  pages        = {10502--10506},
  title        = {Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation},
  url          = {http://dx.doi.org/10.1073/pnas.91.22.10502},
  volume       = {91},
  year         = {1994},
}

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