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Reduced expression of maturation markers in bovine dendritic cells after incubation with Giardia duodenalis trophozoites

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Abstract
Giardia duodenalis (syn. G. intestinalis, G. lamblia) is an important intestinal parasite in a wide range of mammals, including farm animals, companion animals and humans. The role of dendritic cells in the immune response against G. duodenalis is poorly documented and has only been studied in the mouse, which is not a natural host for this parasite. The objective of this study was to investigate whether G. duodenalis trophozoites or their excretion/secretion (ES) products activate and mature dendritic cells from cattle in vitro. Bovine monocyte-derived dendritic cells (MoDCs) were incubated with different concentrations of G. duodenalis trophozoites. An effect of ES products was investigated by preventing direct contact between the trophozoites and the MoDCs, using 0.4μm cell culture inserts. After 18h of stimulation, expression of the maturation markers CD80, CD40 and MHCII was measured and ovalbumin uptake was monitored. A dose-dependent decrease of ovalbumin uptake was observed in MoDCs incubated with trophozoites (but not ES), suggesting functional maturation. However, none of the maturation markers were upregulated after incubation of MoDCs with Giardia trophozoites or ES. In order to investigate whether or not MoDCs can induce lymphocyte proliferation after incubation with Giardia trophozoites, a mixed lymphocyte reaction (MLR) will be performed with allogenic CD2+ lymphocytes. A selection of cytokines will be determined by ELISA in the culture supernatant of the stimulated MoDCs before and after incubation with CD2+ lymphocytes, to determine the phenotype of the immune response that is induced.
Keywords
Giardia duodenalis, bovine, dendritic cells

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Chicago
Grit, Grietje, Bert Devriendt, Thomas Geurden, Ely Bénéré, Jayne Hope, Peter Geldhof, Eric Cox, Jozef Vercruysse, Louis Maes, and Edwin Claerebout. 2011. “Reduced Expression of Maturation Markers in Bovine Dendritic Cells After Incubation with Giardia Duodenalis Trophozoites.” In European Macrophage and Dendritic Cell Society, 25th Annual Meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS).
APA
Grit, G., Devriendt, B., Geurden, T., Bénéré, E., Hope, J., Geldhof, P., Cox, E., et al. (2011). Reduced expression of maturation markers in bovine dendritic cells after incubation with Giardia duodenalis trophozoites. European Macrophage and Dendritic Cell Society, 25th Annual meeting, Abstracts. Presented at the 25th Annual EMDS meeting : Clinical and fundamental aspects of monocyte, macrophage and DC plasticity, European Macrophage and Dendritic cell Society (EMDS).
Vancouver
1.
Grit G, Devriendt B, Geurden T, Bénéré E, Hope J, Geldhof P, et al. Reduced expression of maturation markers in bovine dendritic cells after incubation with Giardia duodenalis trophozoites. European Macrophage and Dendritic Cell Society, 25th Annual meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS); 2011.
MLA
Grit, Grietje, Bert Devriendt, Thomas Geurden, et al. “Reduced Expression of Maturation Markers in Bovine Dendritic Cells After Incubation with Giardia Duodenalis Trophozoites.” European Macrophage and Dendritic Cell Society, 25th Annual Meeting, Abstracts. European Macrophage and Dendritic cell Society (EMDS), 2011. Print.
@inproceedings{1998095,
  abstract     = {Giardia duodenalis (syn. G. intestinalis, G. lamblia) is an important intestinal parasite in a wide range of mammals, including farm animals, companion animals and humans. The role of dendritic cells in the immune response against G. duodenalis is poorly documented and has only been studied in the mouse, which is not a natural host for this parasite. The objective of this study was to investigate whether G. duodenalis trophozoites or their excretion/secretion (ES) products activate and mature dendritic cells from cattle in vitro. Bovine monocyte-derived dendritic cells (MoDCs) were incubated with different concentrations of G. duodenalis trophozoites. An effect of ES products was investigated by preventing direct contact between the trophozoites and the MoDCs, using 0.4\ensuremath{\mu}m cell culture inserts. After 18h of stimulation, expression of the maturation markers CD80, CD40 and MHCII was measured and ovalbumin uptake was monitored. A dose-dependent decrease of ovalbumin uptake was observed in MoDCs incubated with trophozoites (but not ES), suggesting functional maturation. However, none of the maturation markers were upregulated after incubation of MoDCs with Giardia trophozoites or ES. In order to investigate whether or not MoDCs can induce lymphocyte proliferation after incubation with Giardia trophozoites, a mixed lymphocyte reaction (MLR) will be performed with allogenic CD2+ lymphocytes. A selection of cytokines will be determined by ELISA in the culture supernatant of the stimulated MoDCs before and after incubation with CD2+ lymphocytes, to determine the phenotype of the immune response that is induced.},
  author       = {Grit, Grietje and Devriendt, Bert and Geurden, Thomas and B{\'e}n{\'e}r{\'e}, Ely and Hope, Jayne and Geldhof, Peter and Cox, Eric and Vercruysse, Jozef and Maes, Louis and Claerebout, Edwin},
  booktitle    = {European Macrophage and Dendritic Cell Society, 25th Annual meeting, Abstracts},
  keyword      = {Giardia duodenalis,bovine,dendritic cells},
  language     = {eng},
  location     = {Brussels, Belgium},
  publisher    = {European Macrophage and Dendritic cell Society (EMDS)},
  title        = {Reduced expression of maturation markers in bovine dendritic cells after incubation with Giardia duodenalis trophozoites},
  year         = {2011},
}