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Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3)

Hendrik Waegeman (UGent) , Jo Maertens (UGent) , Joeri Beauprez (UGent) , Marjan De Mey (UGent) and Wim Soetaert (UGent)
(2012) BIOTECHNOLOGY LETTERS. 34(2). p.329-337
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO2 yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double knockout strain E. coli BL21 (DE3) DarcADiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose concentrations (10–15 g l-1), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared to in E. coli K12.
Keywords
Acetate, ArcA, BL21, Escherichia coli, IclR, Recombinant protein production, CATABOLITE REPRESSION MUTANTS, SUGAR MIXTURES, PHOSPHOTRANSFERASE SYSTEM, GLUCOSE-UTILIZATION, GLYOXYLATE SHUNT, GROWTH-RATES, ACETATE, FERMENTATION, K-12, EXPRESSION

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Chicago
Waegeman, Hendrik, Jo Maertens, Joeri Beauprez, Marjan De Mey, and Wim Soetaert. 2012. “Effect of iclR and arcA Deletions on Physiology and Metabolic Fluxes in Escherichia Coli BL21 (DE3).” Biotechnology Letters 34 (2): 329–337.
APA
Waegeman, H., Maertens, J., Beauprez, J., De Mey, M., & Soetaert, W. (2012). Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3). BIOTECHNOLOGY LETTERS, 34(2), 329–337.
Vancouver
1.
Waegeman H, Maertens J, Beauprez J, De Mey M, Soetaert W. Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3). BIOTECHNOLOGY LETTERS. 2012;34(2):329–37.
MLA
Waegeman, Hendrik, Jo Maertens, Joeri Beauprez, et al. “Effect of iclR and arcA Deletions on Physiology and Metabolic Fluxes in Escherichia Coli BL21 (DE3).” BIOTECHNOLOGY LETTERS 34.2 (2012): 329–337. Print.
@article{1997635,
  abstract     = {Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70\%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO2 yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double knockout strain E. coli BL21 (DE3) DarcADiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose concentrations (10--15 g l-1), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared to in E. coli K12.},
  author       = {Waegeman, Hendrik and Maertens, Jo and Beauprez, Joeri and De Mey, Marjan and Soetaert, Wim},
  issn         = {0141-5492},
  journal      = {BIOTECHNOLOGY LETTERS},
  keyword      = {Acetate,ArcA,BL21,Escherichia coli,IclR,Recombinant protein production,CATABOLITE REPRESSION MUTANTS,SUGAR MIXTURES,PHOSPHOTRANSFERASE SYSTEM,GLUCOSE-UTILIZATION,GLYOXYLATE SHUNT,GROWTH-RATES,ACETATE,FERMENTATION,K-12,EXPRESSION},
  language     = {eng},
  number       = {2},
  pages        = {329--337},
  title        = {Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3)},
  url          = {http://dx.doi.org/10.1007/s10529-011-0774-6},
  volume       = {34},
  year         = {2012},
}

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