Ghent University Academic Bibliography

Advanced

Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3)

Hendrik Waegeman, Jo Maertens UGent, Joeri Beauprez, Marjan De Mey UGent and Wim Soetaert UGent (2012) BIOTECHNOLOGY LETTERS. 34(2). p.329-337
abstract
Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO2 yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double knockout strain E. coli BL21 (DE3) DarcADiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose concentrations (10–15 g l-1), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared to in E. coli K12.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Acetate, ArcA, BL21, Escherichia coli, IclR, Recombinant protein production, CATABOLITE REPRESSION MUTANTS, SUGAR MIXTURES, PHOSPHOTRANSFERASE SYSTEM, GLUCOSE-UTILIZATION, GLYOXYLATE SHUNT, GROWTH-RATES, ACETATE, FERMENTATION, K-12, EXPRESSION
journal title
BIOTECHNOLOGY LETTERS
Biotechnol. Lett.
volume
34
issue
2
pages
329 - 337
Web of Science type
Article
Web of Science id
000299510200021
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
1.853 (2012)
JCR rank
91/157 (2012)
JCR quartile
3 (2012)
ISSN
0141-5492
DOI
10.1007/s10529-011-0774-6
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1997635
handle
http://hdl.handle.net/1854/LU-1997635
date created
2012-01-20 09:08:06
date last changed
2016-12-19 15:42:54
@article{1997635,
  abstract     = {Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70\%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO2 yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double knockout strain E. coli BL21 (DE3) DarcADiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose concentrations (10--15 g l-1), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared to in E. coli K12.},
  author       = {Waegeman, Hendrik and Maertens, Jo and Beauprez, Joeri and De Mey, Marjan and Soetaert, Wim},
  issn         = {0141-5492},
  journal      = {BIOTECHNOLOGY LETTERS},
  keyword      = {Acetate,ArcA,BL21,Escherichia coli,IclR,Recombinant protein production,CATABOLITE REPRESSION MUTANTS,SUGAR MIXTURES,PHOSPHOTRANSFERASE SYSTEM,GLUCOSE-UTILIZATION,GLYOXYLATE SHUNT,GROWTH-RATES,ACETATE,FERMENTATION,K-12,EXPRESSION},
  language     = {eng},
  number       = {2},
  pages        = {329--337},
  title        = {Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3)},
  url          = {http://dx.doi.org/10.1007/s10529-011-0774-6},
  volume       = {34},
  year         = {2012},
}

Chicago
Waegeman, Hendrik, Jo Maertens, Joeri Beauprez, Marjan De Mey, and Wim Soetaert. 2012. “Effect of iclR and arcA Deletions on Physiology and Metabolic Fluxes in Escherichia Coli BL21 (DE3).” Biotechnology Letters 34 (2): 329–337.
APA
Waegeman, H., Maertens, J., Beauprez, J., De Mey, M., & Soetaert, W. (2012). Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3). BIOTECHNOLOGY LETTERS, 34(2), 329–337.
Vancouver
1.
Waegeman H, Maertens J, Beauprez J, De Mey M, Soetaert W. Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3). BIOTECHNOLOGY LETTERS. 2012;34(2):329–37.
MLA
Waegeman, Hendrik, Jo Maertens, Joeri Beauprez, et al. “Effect of iclR and arcA Deletions on Physiology and Metabolic Fluxes in Escherichia Coli BL21 (DE3).” BIOTECHNOLOGY LETTERS 34.2 (2012): 329–337. Print.