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Salvage of oocytes from sterile genetically valuable cows, resulting in the birth of a calf

(1994) ANIMAL REPRODUCTION SCIENCE. 36(3-4). p.187-196
Author
Organization
Abstract
The objective of this study was to determine the possible application of in vitro fertilization techniques for calf production in incurable sterility patients or in involuntary cull cows. Ten sterile or fatally injured dams of high genetic value were submitted to in vitro fertilization procedures. Ovaries were recovered after slaughter in eight animals or after ovariectomy in two cows. A total of 261 oocytes were obtained and matured in vitro. Frozen-thawed sperm from eight different bulls of high breeding value (selected to match with one particular cow) was separated with Percoll and used for in vitro fertilization in the presence of heparin. Seventy four embryos started to cleave and 7 days post-insemination, 13 transferable embryos were obtained. Eleven embryos were transferred fresh into nine recipients and produced two pregnancies. One pregnant recipient died of hydro-allantois, the other delivered a live bull calf 288 days after in vitro fertilization.
Keywords
SLAUGHTERED COWS, EMBRYOS PRODUCED INVITRO, BOVINE OOCYTES, FERTILIZATION, OVARIES, CATTLE, CALVES

Citation

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Chicago
Van Soom, Ann, I Van Vlaenderen, AR Mahmoudzadeh, Maria Ysebaert, and Aart de Kruif. 1994. “Salvage of Oocytes from Sterile Genetically Valuable Cows, Resulting in the Birth of a Calf.” Animal Reproduction Science 36 (3-4): 187–196.
APA
Van Soom, A., Van Vlaenderen, I., Mahmoudzadeh, A., Ysebaert, M., & de Kruif, A. (1994). Salvage of oocytes from sterile genetically valuable cows, resulting in the birth of a calf. ANIMAL REPRODUCTION SCIENCE, 36(3-4), 187–196.
Vancouver
1.
Van Soom A, Van Vlaenderen I, Mahmoudzadeh A, Ysebaert M, de Kruif A. Salvage of oocytes from sterile genetically valuable cows, resulting in the birth of a calf. ANIMAL REPRODUCTION SCIENCE. 1994;36(3-4):187–96.
MLA
Van Soom, Ann, I Van Vlaenderen, AR Mahmoudzadeh, et al. “Salvage of Oocytes from Sterile Genetically Valuable Cows, Resulting in the Birth of a Calf.” ANIMAL REPRODUCTION SCIENCE 36.3-4 (1994): 187–196. Print.
@article{199699,
  abstract     = {The objective of this study was to determine the possible application of in vitro fertilization techniques for calf production in incurable sterility patients or in involuntary cull cows. Ten sterile or fatally injured dams of high genetic value were submitted to in vitro fertilization procedures. Ovaries were recovered after slaughter in eight animals or after ovariectomy in two cows. A total of 261 oocytes were obtained and matured in vitro. Frozen-thawed sperm from eight different bulls of high breeding value (selected to match with one particular cow) was separated with Percoll and used for in vitro fertilization in the presence of heparin. Seventy four embryos started to cleave and 7 days post-insemination, 13 transferable embryos were obtained. Eleven embryos were transferred fresh into nine recipients and produced two pregnancies. One pregnant recipient died of hydro-allantois, the other delivered a live bull calf 288 days after in vitro fertilization.},
  author       = {Van Soom, Ann and Van Vlaenderen, I and Mahmoudzadeh, AR and Ysebaert, Maria and de Kruif, Aart},
  issn         = {0378-4320},
  journal      = {ANIMAL REPRODUCTION SCIENCE},
  keyword      = {SLAUGHTERED COWS,EMBRYOS PRODUCED INVITRO,BOVINE OOCYTES,FERTILIZATION,OVARIES,CATTLE,CALVES},
  language     = {eng},
  number       = {3-4},
  pages        = {187--196},
  title        = {Salvage of oocytes from sterile genetically valuable cows, resulting in the birth of a calf},
  url          = {http://dx.doi.org/10.1016/0378-4320(94)90067-1},
  volume       = {36},
  year         = {1994},
}

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