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Quorum sensing regulation of virulence gene expression in Vibrio harveyi in vitro and in vivo during infection of gnotobiotic brine shrimp larvae

Darshanee Ruwandeepika Hettipala Arachchige UGent, Patit Paban Bhowmick, Indrani Karunasagar, Peter Bossier UGent and Tom Defoirdt UGent (2011) ENVIRONMENTAL MICROBIOLOGY REPORTS. 3(5). p.597-602
abstract
In this study, we investigated quorum sensing regulation of virulence genes in Vibrio harveyi by determining their expression levels, both in vitro and in vivo during infection of gnotobiotic brine shrimp. The quorum sensing master regulator luxR and the vhp metalloprotease showed around threefold and fivefold higher expression levels in a luxO mutant with maximum quorum sensing activity than in a luxO mutant with minimum quorum sensing activity. There was no difference in expression of the vhh haemolysin gene between the two mutants. There was however more than 2.5-fold lower expression in an AI-2-negative mutant, suggesting that this gene is specifically regulated by AI-2 quorum sensing through a yet unknown signal transduction cascade. The in vivo expression data showed a peak in expression of the quorum sensing master regulator luxR and the vhp metalloprotease after 24 h of incubation in wild-type V. harveyi and the luxO mutant mimicking a maximally activated quorum sensing system, whereas the expression remained low in the luxO mutant mimicking a completely inactivated quorum sensing system. The vhh haemolysin gene showed a peak in expression after 24 h in the wild type and a constantly low expression in an AI-2-negative mutant.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARTEMIA-FRANCISCANA, STRUCTURAL IDENTIFICATION, LUXR GENE, LUMINESCENCE, AUTOINDUCER, SYSTEM, ANGUILLARUM, VULNIFICUS, PATHOGEN, HOMOLOG
journal title
ENVIRONMENTAL MICROBIOLOGY REPORTS
Environ. Microbiol. Rep.
volume
3
issue
5
pages
597 - 602
Web of Science type
Article
Web of Science id
000297148600013
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
3.232 (2011)
JCR rank
31/203 (2011)
JCR quartile
1 (2011)
ISSN
1758-2229
DOI
10.1111/j.1758-2229.2011.00268.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1993082
handle
http://hdl.handle.net/1854/LU-1993082
date created
2012-01-18 13:19:36
date last changed
2016-12-19 15:42:53
@article{1993082,
  abstract     = {In this study, we investigated quorum sensing regulation of virulence genes in Vibrio harveyi by determining their expression levels, both in vitro and in vivo during infection of gnotobiotic brine shrimp. The quorum sensing master regulator luxR and the vhp metalloprotease showed around threefold and fivefold higher expression levels in a luxO mutant with maximum quorum sensing activity than in a luxO mutant with minimum quorum sensing activity. There was no difference in expression of the vhh haemolysin gene between the two mutants. There was however more than 2.5-fold lower expression in an AI-2-negative mutant, suggesting that this gene is specifically regulated by AI-2 quorum sensing through a yet unknown signal transduction cascade. The in vivo expression data showed a peak in expression of the quorum sensing master regulator luxR and the vhp metalloprotease after 24 h of incubation in wild-type V. harveyi and the luxO mutant mimicking a maximally activated quorum sensing system, whereas the expression remained low in the luxO mutant mimicking a completely inactivated quorum sensing system. The vhh haemolysin gene showed a peak in expression after 24 h in the wild type and a constantly low expression in an AI-2-negative mutant.},
  author       = {Ruwandeepika Hettipala Arachchige, Darshanee and Bhowmick, Patit Paban and Karunasagar, Indrani and Bossier, Peter and Defoirdt, Tom},
  issn         = {1758-2229},
  journal      = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
  keyword      = {ARTEMIA-FRANCISCANA,STRUCTURAL IDENTIFICATION,LUXR GENE,LUMINESCENCE,AUTOINDUCER,SYSTEM,ANGUILLARUM,VULNIFICUS,PATHOGEN,HOMOLOG},
  language     = {eng},
  number       = {5},
  pages        = {597--602},
  title        = {Quorum sensing regulation of virulence gene expression in Vibrio harveyi in vitro and in vivo during infection of gnotobiotic brine shrimp larvae},
  url          = {http://dx.doi.org/10.1111/j.1758-2229.2011.00268.x},
  volume       = {3},
  year         = {2011},
}

Chicago
Ruwandeepika Hettipala Arachchige, Darshanee, Patit Paban Bhowmick, Indrani Karunasagar, Peter Bossier, and Tom Defoirdt. 2011. “Quorum Sensing Regulation of Virulence Gene Expression in Vibrio Harveyi in Vitro and in Vivo During Infection of Gnotobiotic Brine Shrimp Larvae.” Environmental Microbiology Reports 3 (5): 597–602.
APA
Ruwandeepika Hettipala Arachchige, D., Bhowmick, P. P., Karunasagar, I., Bossier, P., & Defoirdt, T. (2011). Quorum sensing regulation of virulence gene expression in Vibrio harveyi in vitro and in vivo during infection of gnotobiotic brine shrimp larvae. ENVIRONMENTAL MICROBIOLOGY REPORTS, 3(5), 597–602.
Vancouver
1.
Ruwandeepika Hettipala Arachchige D, Bhowmick PP, Karunasagar I, Bossier P, Defoirdt T. Quorum sensing regulation of virulence gene expression in Vibrio harveyi in vitro and in vivo during infection of gnotobiotic brine shrimp larvae. ENVIRONMENTAL MICROBIOLOGY REPORTS. 2011;3(5):597–602.
MLA
Ruwandeepika Hettipala Arachchige, Darshanee, Patit Paban Bhowmick, Indrani Karunasagar, et al. “Quorum Sensing Regulation of Virulence Gene Expression in Vibrio Harveyi in Vitro and in Vivo During Infection of Gnotobiotic Brine Shrimp Larvae.” ENVIRONMENTAL MICROBIOLOGY REPORTS 3.5 (2011): 597–602. Print.