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Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures

EG Velliou, E Van Derlinden, AM Cappuyns, D Aerts, E Nikolaidou, AH Geeraerd, Frank Devlieghere UGent and JF Van Impe (2011) LETTERS IN APPLIED MICROBIOLOGY. 52(2). p.116-122
abstract
Aim: To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. Methods and Results: Stationary-phase E. coli cells were inactivated at 52, 54 and 58 degrees C. The heat resistance is described as reduction in the inactivation rate, k(max), and/or an increase in the time for one decimal reduction, D, and/or an increase in the time for the fourth decimal reduction, t(4D). Conclusions: Resistance of E. coli changed - increased - at all temperatures under study. Generally, the addition of TMAO to the growth medium protected E. coli cells, leading to an increase in their heat resistance, i.e. reduced k(max) and increased D and t(4D) values are obtained. Significance and Impact of the Study: Additional knowledge on the reaction of E. coli to heat in the presence of the organic osmolyte TMAO at lethal temperatures is provided. This work contributes to an improved understanding of the level of the resistance of bacteria to heat in the presence of osmolytes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
microbial stress, molecular chaperones, TMAO, osmolytes, inactivation, heat resistance, Escherichia coli K12, CHEMICAL CHAPERONES, INACTIVATION
journal title
LETTERS IN APPLIED MICROBIOLOGY
Lett. Appl. Microbiol.
volume
52
issue
2
pages
116 - 122
Web of Science type
Article
Web of Science id
000286003400005
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
1.622 (2011)
JCR rank
101/157 (2011)
JCR quartile
3 (2011)
ISSN
0266-8254
DOI
10.1111/j.1472-765X.2010.02974.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1993025
handle
http://hdl.handle.net/1854/LU-1993025
date created
2012-01-18 13:17:30
date last changed
2016-12-19 15:42:53
@article{1993025,
  abstract     = {Aim: To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures.
Methods and Results: Stationary-phase E. coli cells were inactivated at 52, 54 and 58 degrees C. The heat resistance is described as reduction in the inactivation rate, k(max), and/or an increase in the time for one decimal reduction, D, and/or an increase in the time for the fourth decimal reduction, t(4D).
Conclusions: Resistance of E. coli changed - increased - at all temperatures under study. Generally, the addition of TMAO to the growth medium protected E. coli cells, leading to an increase in their heat resistance, i.e. reduced k(max) and increased D and t(4D) values are obtained.
Significance and Impact of the Study: Additional knowledge on the reaction of E. coli to heat in the presence of the organic osmolyte TMAO at lethal temperatures is provided. This work contributes to an improved understanding of the level of the resistance of bacteria to heat in the presence of osmolytes.},
  author       = {Velliou, EG and Van Derlinden, E and Cappuyns, AM and Aerts, D and Nikolaidou, E and Geeraerd, AH and Devlieghere, Frank and Van Impe, JF},
  issn         = {0266-8254},
  journal      = {LETTERS IN APPLIED MICROBIOLOGY},
  keyword      = {microbial stress,molecular chaperones,TMAO,osmolytes,inactivation,heat resistance,Escherichia coli K12,CHEMICAL CHAPERONES,INACTIVATION},
  language     = {eng},
  number       = {2},
  pages        = {116--122},
  title        = {Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures},
  url          = {http://dx.doi.org/10.1111/j.1472-765X.2010.02974.x},
  volume       = {52},
  year         = {2011},
}

Chicago
Velliou, EG, E Van Derlinden, AM Cappuyns, D Aerts, E Nikolaidou, AH Geeraerd, Frank Devlieghere, and JF Van Impe. 2011. “Quantification of the Influence of trimethylamine-N-oxide (TMAO) on the Heat Resistance of Escherichia Coli K12 at Lethal Temperatures.” Letters in Applied Microbiology 52 (2): 116–122.
APA
Velliou, E., Van Derlinden, E., Cappuyns, A., Aerts, D., Nikolaidou, E., Geeraerd, A., Devlieghere, F., et al. (2011). Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures. LETTERS IN APPLIED MICROBIOLOGY, 52(2), 116–122.
Vancouver
1.
Velliou E, Van Derlinden E, Cappuyns A, Aerts D, Nikolaidou E, Geeraerd A, et al. Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures. LETTERS IN APPLIED MICROBIOLOGY. 2011;52(2):116–22.
MLA
Velliou, EG, E Van Derlinden, AM Cappuyns, et al. “Quantification of the Influence of trimethylamine-N-oxide (TMAO) on the Heat Resistance of Escherichia Coli K12 at Lethal Temperatures.” LETTERS IN APPLIED MICROBIOLOGY 52.2 (2011): 116–122. Print.