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Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures

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Abstract
Aim: To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. Methods and Results: Stationary-phase E. coli cells were inactivated at 52, 54 and 58 degrees C. The heat resistance is described as reduction in the inactivation rate, k(max), and/or an increase in the time for one decimal reduction, D, and/or an increase in the time for the fourth decimal reduction, t(4D). Conclusions: Resistance of E. coli changed - increased - at all temperatures under study. Generally, the addition of TMAO to the growth medium protected E. coli cells, leading to an increase in their heat resistance, i.e. reduced k(max) and increased D and t(4D) values are obtained. Significance and Impact of the Study: Additional knowledge on the reaction of E. coli to heat in the presence of the organic osmolyte TMAO at lethal temperatures is provided. This work contributes to an improved understanding of the level of the resistance of bacteria to heat in the presence of osmolytes.
Keywords
microbial stress, molecular chaperones, TMAO, osmolytes, inactivation, heat resistance, Escherichia coli K12, CHEMICAL CHAPERONES, INACTIVATION

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Chicago
Velliou, EG, E Van Derlinden, AM Cappuyns, D Aerts, E Nikolaidou, AH Geeraerd, Frank Devlieghere, and JF Van Impe. 2011. “Quantification of the Influence of trimethylamine-N-oxide (TMAO) on the Heat Resistance of Escherichia Coli K12 at Lethal Temperatures.” Letters in Applied Microbiology 52 (2): 116–122.
APA
Velliou, E., Van Derlinden, E., Cappuyns, A., Aerts, D., Nikolaidou, E., Geeraerd, A., Devlieghere, F., et al. (2011). Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures. LETTERS IN APPLIED MICROBIOLOGY, 52(2), 116–122.
Vancouver
1.
Velliou E, Van Derlinden E, Cappuyns A, Aerts D, Nikolaidou E, Geeraerd A, et al. Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures. LETTERS IN APPLIED MICROBIOLOGY. 2011;52(2):116–22.
MLA
Velliou, EG, E Van Derlinden, AM Cappuyns, et al. “Quantification of the Influence of trimethylamine-N-oxide (TMAO) on the Heat Resistance of Escherichia Coli K12 at Lethal Temperatures.” LETTERS IN APPLIED MICROBIOLOGY 52.2 (2011): 116–122. Print.
@article{1993025,
  abstract     = {Aim: To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures.
Methods and Results: Stationary-phase E. coli cells were inactivated at 52, 54 and 58 degrees C. The heat resistance is described as reduction in the inactivation rate, k(max), and/or an increase in the time for one decimal reduction, D, and/or an increase in the time for the fourth decimal reduction, t(4D).
Conclusions: Resistance of E. coli changed - increased - at all temperatures under study. Generally, the addition of TMAO to the growth medium protected E. coli cells, leading to an increase in their heat resistance, i.e. reduced k(max) and increased D and t(4D) values are obtained.
Significance and Impact of the Study: Additional knowledge on the reaction of E. coli to heat in the presence of the organic osmolyte TMAO at lethal temperatures is provided. This work contributes to an improved understanding of the level of the resistance of bacteria to heat in the presence of osmolytes.},
  author       = {Velliou, EG and Van Derlinden, E and Cappuyns, AM and Aerts, D and Nikolaidou, E and Geeraerd, AH and Devlieghere, Frank and Van Impe, JF},
  issn         = {0266-8254},
  journal      = {LETTERS IN APPLIED MICROBIOLOGY},
  keyword      = {microbial stress,molecular chaperones,TMAO,osmolytes,inactivation,heat resistance,Escherichia coli K12,CHEMICAL CHAPERONES,INACTIVATION},
  language     = {eng},
  number       = {2},
  pages        = {116--122},
  title        = {Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures},
  url          = {http://dx.doi.org/10.1111/j.1472-765X.2010.02974.x},
  volume       = {52},
  year         = {2011},
}

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