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Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence

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Abstract
A fish pathogen Edwardsiella tarda LTB-4 produced various indole alkaloids, including indole, 2-(1H-indol-3-yl)ethanol, 4-di(1H-indol-3-yl) methylphenol, tri(1H-indol-3-yl) methane and 2-[2,2-bis(1H-indol-3-yl)] ethylphenylamine. Indole was the most abundant among these indole alkaloids. E. tarda LTB-4 produced indoles during its whole growth phase and maintained a high level (around 35.5 mu M) during the stationary phase. The relevant tryptophanase (TnaA) gene tnaA was cloned from LTB-4 and conditionally expressed in Escherichia coli; the recombinant TnaA catalysed L-tryptophan to indole. A tnaA in-frame deletion mutant Delta tnaA was constructed through double cross-over allelic exchange by means of the suicide vector pRE118; deletion of tnaA caused some phenotypic changes including decreased swarming and twitching motility, lipopolysaccharide production and multiple antibiotic resistances. Also, subtherapeutic doses of chloromycetin, carbenicillin and tetracyline could cause the decrease of bacterial growth, but greatly induce the production of indole by E. tarda. Most importantly, attenuated virulence of the Delta tnaA mutant to zebra fish by increasing the LD(50) for about 55-fold indicated that TnaA involved in the virulence of E. tarda.
Keywords
SENSING SIGNAL MOLECULES, PSEUDOMONAS-AERUGINOSA PAO1, ESCHERICHIA-COLI K-12, BIOFILM FORMATION, EXTRACELLULAR SIGNAL, SWARMING MOTILITY, VIBRIO-CHOLERAE, INDOLE, EXPRESSION, IDENTIFICATION

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Chicago
Han, Yin, Chun-Li Yang, Qian Yang, Zizhong Qi, Wenzheng Liu, Zhi-Hong Xu, Wei-Ming Zhu, Peter Bossier, and Xiao-Hua Zhang. 2011. “Mutation of Tryptophanase Gene tnaA in Edwardsiella Tarda Reduces Lipopolysaccharide Production, Antibiotic Resistance and Virulence.” Environmental Microbiology Reports 3 (5): 603–612.
APA
Han, Y., Yang, C.-L., Yang, Q., Qi, Z., Liu, W., Xu, Z.-H., Zhu, W.-M., et al. (2011). Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence. ENVIRONMENTAL MICROBIOLOGY REPORTS, 3(5), 603–612.
Vancouver
1.
Han Y, Yang C-L, Yang Q, Qi Z, Liu W, Xu Z-H, et al. Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence. ENVIRONMENTAL MICROBIOLOGY REPORTS. 2011;3(5):603–12.
MLA
Han, Yin, Chun-Li Yang, Qian Yang, et al. “Mutation of Tryptophanase Gene tnaA in Edwardsiella Tarda Reduces Lipopolysaccharide Production, Antibiotic Resistance and Virulence.” ENVIRONMENTAL MICROBIOLOGY REPORTS 3.5 (2011): 603–612. Print.
@article{1992206,
  abstract     = {A fish pathogen Edwardsiella tarda LTB-4 produced various indole alkaloids, including indole, 2-(1H-indol-3-yl)ethanol, 4-di(1H-indol-3-yl) methylphenol, tri(1H-indol-3-yl) methane and 2-[2,2-bis(1H-indol-3-yl)] ethylphenylamine. Indole was the most abundant among these indole alkaloids. E. tarda LTB-4 produced indoles during its whole growth phase and maintained a high level (around 35.5 mu M) during the stationary phase. The relevant tryptophanase (TnaA) gene tnaA was cloned from LTB-4 and conditionally expressed in Escherichia coli; the recombinant TnaA catalysed L-tryptophan to indole. A tnaA in-frame deletion mutant Delta tnaA was constructed through double cross-over allelic exchange by means of the suicide vector pRE118; deletion of tnaA caused some phenotypic changes including decreased swarming and twitching motility, lipopolysaccharide production and multiple antibiotic resistances. Also, subtherapeutic doses of chloromycetin, carbenicillin and tetracyline could cause the decrease of bacterial growth, but greatly induce the production of indole by E. tarda. Most importantly, attenuated virulence of the Delta tnaA mutant to zebra fish by increasing the LD(50) for about 55-fold indicated that TnaA involved in the virulence of E. tarda.},
  author       = {Han, Yin and Yang, Chun-Li and Yang, Qian and Qi, Zizhong and Liu, Wenzheng and Xu, Zhi-Hong and Zhu, Wei-Ming and Bossier, Peter and Zhang, Xiao-Hua},
  issn         = {1758-2229},
  journal      = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
  keyword      = {SENSING SIGNAL MOLECULES,PSEUDOMONAS-AERUGINOSA PAO1,ESCHERICHIA-COLI K-12,BIOFILM FORMATION,EXTRACELLULAR SIGNAL,SWARMING MOTILITY,VIBRIO-CHOLERAE,INDOLE,EXPRESSION,IDENTIFICATION},
  language     = {eng},
  number       = {5},
  pages        = {603--612},
  title        = {Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence},
  url          = {http://dx.doi.org/10.1111/j.1758-2229.2011.00269.x},
  volume       = {3},
  year         = {2011},
}

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