Advanced search
1 file | 1.15 MB
Author
Organization
Abstract
We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 mu g/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.
Keywords
SERUM, KIDNEY, IDENTIFICATION, UROMODULIN, HUMAN-PREGNANCY URINE, HUMAN URINARY PROTEIN, TAMM-HORSFALL GLYCOPROTEIN, LEWIS CARCINOMA, LISTERIA MONOCYTOGENES, MACROPHAGES, SCID MICE, URINE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.15 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Fontan, Elisabeth, Emmanuel Briend, Hélène Saklani-Jusforgues, Jacques d’ Alayer, Joël Vandekerckhove, and Robert M Fauve. 1994. “A 92-kDa Human Immunostimulatory Protein.” Proceedings of the National Academy of Sciences of the United States of America 91 (18): 8353–8357.
APA
Fontan, E., Briend, E., Saklani-Jusforgues, H., d’ Alayer, J., Vandekerckhove, J., & Fauve, R. M. (1994). A 92-kDa human immunostimulatory protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91(18), 8353–8357.
Vancouver
1.
Fontan E, Briend E, Saklani-Jusforgues H, d’ Alayer J, Vandekerckhove J, Fauve RM. A 92-kDa human immunostimulatory protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1994;91(18):8353–7.
MLA
Fontan, Elisabeth, Emmanuel Briend, Hélène Saklani-Jusforgues, et al. “A 92-kDa Human Immunostimulatory Protein.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 91.18 (1994): 8353–8357. Print.
@article{199137,
  abstract     = {We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 mu g/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety.},
  author       = {Fontan, Elisabeth and Briend, Emmanuel and Saklani-Jusforgues, H{\'e}l{\`e}ne and d'Alayer, Jacques and Vandekerckhove, Jo{\"e}l and Fauve, Robert M},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {SERUM,KIDNEY,IDENTIFICATION,UROMODULIN,HUMAN-PREGNANCY URINE,HUMAN URINARY PROTEIN,TAMM-HORSFALL GLYCOPROTEIN,LEWIS CARCINOMA,LISTERIA MONOCYTOGENES,MACROPHAGES,SCID MICE,URINE},
  language     = {eng},
  number       = {18},
  pages        = {8353--8357},
  title        = {A 92-kDa human immunostimulatory protein},
  url          = {http://dx.doi.org/10.1073/pnas.91.18.8353},
  volume       = {91},
  year         = {1994},
}

Altmetric
View in Altmetric