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Waterborne versus dietary zinc accumulation and toxicity in Daphnia magna: a synchrotron radiation based x-ray fluorescence imaging approach

Roel Evens UGent, Karel De Schamphelaere UGent, Björn De Samber UGent, Geert Silversmit UGent, Tom Schoonjans UGent, Bart Vekemans UGent, Lieve Balcaen UGent, Frank Vanhaecke UGent, I Szaloki and K Rickers, et al. (2012) ENVIRONMENTAL SCIENCE & TECHNOLOGY. 46(2). p.1178-1184
abstract
Recent studies have suggested that exposure of the freshwater invertebrate Daphnia magna to dietary Zn may selectively affect reproduction without an associated increase of whole body bioaccumulation of Zn. The aim of the current research was therefore to investigate the hypothesis that dietary Zn toxicity is the result of selective accumulation in tissues that are directly involved in reproduction. Since under field conditions simultaneous exposure to both waterborne and dietary Zn is likely to occur, it was also tested if accumulation and toxicity under combined waterborne and dietary Zn exposure is the result of interactive effects. To this purpose, D. magna was exposed during a 16-day reproduction assay to Zn following a 5 × 2 factorial design, comprising five waterborne concentrations (12, 65, 137, 207, and 281 μg Zn/L) and two dietary Zn levels (49.6 and 495.9 μg Zn/g dry wt.). Tissuespecific Zn distribution was quantified by synchrotron radiation based confocal X-ray fluorescence (XRF). It was observed that the occurrence of reproductive inhibition due to increasing waterborne Zn exposure (from 65 μg/L to 281 μg/L) was accompanied by a relative increase of the Zn burdens which was similar in all tissues considered (i.e., the carapax, eggs, thoracic appendages with gills and the cluster comprising gut epithelium, storage cells and ovaries). In contrast, the impairment of reproduction during dietary Zn exposure was accompanied by a clearly discernible Zn accumulation in the eggs only (at 65 μg/L of waterborne Zn). During simultaneous exposure, bioaccumulation and toxicity were the result of interaction, which implies that the tissue-specific bioaccumulation and toxicity following dietary Zn exposure are dependent on the Zn concentration in the water. Our findings emphasize that (i) effects of dietary Zn exposure should preferably not be investigated in isolation from waterborne Zn exposure, and that (ii) XRF enabled us to provide possible links between tissue-specific oaccumulation and reproductive effects of Zn.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXPOSURE, REPRODUCTIVE TOXICITY, CLADOCERA, CRUSTACEA, CADMIUM, CELLS, MODEL, LINKS
journal title
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Environ. Sci. Technol
volume
46
issue
2
pages
1178 - 1184
Web of Science type
Article
Web of Science id
000299136200078
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
5.257 (2012)
JCR rank
7/209 (2012)
JCR quartile
1 (2012)
ISSN
0013-936X
DOI
10.1021/es203140p
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1989220
handle
http://hdl.handle.net/1854/LU-1989220
date created
2012-01-17 11:15:38
date last changed
2012-10-03 16:03:29
@article{1989220,
  abstract     = {Recent studies have suggested that exposure of the freshwater invertebrate Daphnia magna to dietary Zn may selectively affect reproduction without an associated increase of whole body bioaccumulation of Zn. The aim of the current research was therefore to investigate the hypothesis that dietary Zn toxicity is the result of selective accumulation in tissues that are directly involved in reproduction. Since under field conditions simultaneous exposure to both waterborne and dietary Zn is likely to occur, it was also tested if accumulation and toxicity under combined waterborne and dietary Zn exposure is the result of interactive effects. To this purpose, D. magna was exposed during a 16-day reproduction assay to Zn following a 5 {\texttimes} 2 factorial design, comprising five waterborne concentrations (12, 65, 137, 207, and 281 \ensuremath{\mu}g Zn/L) and two dietary Zn levels (49.6 and 495.9 \ensuremath{\mu}g Zn/g dry wt.). Tissuespecific Zn distribution was quantified by synchrotron radiation based confocal X-ray fluorescence (XRF). It was observed that the occurrence of reproductive inhibition due to increasing waterborne Zn exposure (from 65 \ensuremath{\mu}g/L to 281 \ensuremath{\mu}g/L) was accompanied by a relative increase of the Zn burdens which was similar in all tissues considered (i.e., the carapax, eggs, thoracic appendages with gills and the cluster comprising gut epithelium, storage cells and ovaries). In contrast, the impairment of reproduction during dietary Zn exposure was accompanied by a clearly discernible Zn accumulation in the eggs only (at 65 \ensuremath{\mu}g/L of waterborne Zn). During simultaneous exposure, bioaccumulation and toxicity were the result of interaction, which implies that the tissue-specific bioaccumulation and toxicity following dietary Zn exposure are dependent on the Zn concentration in the water. Our findings emphasize that (i) effects of dietary Zn exposure should preferably not be investigated in isolation from waterborne Zn exposure, and that (ii) XRF enabled us to provide possible links between tissue-specific oaccumulation and reproductive effects of Zn.},
  author       = {Evens, Roel and De Schamphelaere, Karel and De Samber, Bj{\"o}rn and Silversmit, Geert and Schoonjans, Tom and Vekemans, Bart and Balcaen, Lieve and Vanhaecke, Frank and Szaloki, I and Rickers, K and Falkenberg, G and Vincze, Laszlo and Janssen, Colin},
  issn         = {0013-936X},
  journal      = {ENVIRONMENTAL SCIENCE \& TECHNOLOGY},
  keyword      = {EXPOSURE,REPRODUCTIVE TOXICITY,CLADOCERA,CRUSTACEA,CADMIUM,CELLS,MODEL,LINKS},
  language     = {eng},
  number       = {2},
  pages        = {1178--1184},
  title        = {Waterborne versus dietary zinc accumulation and toxicity in Daphnia magna: a synchrotron radiation based x-ray fluorescence imaging approach},
  url          = {http://dx.doi.org/10.1021/es203140p},
  volume       = {46},
  year         = {2012},
}

Chicago
Evens, Roel, Karel De Schamphelaere, Björn De Samber, Geert Silversmit, Tom Schoonjans, Bart Vekemans, Lieve Balcaen, et al. 2012. “Waterborne Versus Dietary Zinc Accumulation and Toxicity in Daphnia Magna: a Synchrotron Radiation Based X-ray Fluorescence Imaging Approach.” Environmental Science & Technology 46 (2): 1178–1184.
APA
Evens, R., De Schamphelaere, K., De Samber, B., Silversmit, G., Schoonjans, T., Vekemans, B., Balcaen, L., et al. (2012). Waterborne versus dietary zinc accumulation and toxicity in Daphnia magna: a synchrotron radiation based x-ray fluorescence imaging approach. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 46(2), 1178–1184.
Vancouver
1.
Evens R, De Schamphelaere K, De Samber B, Silversmit G, Schoonjans T, Vekemans B, et al. Waterborne versus dietary zinc accumulation and toxicity in Daphnia magna: a synchrotron radiation based x-ray fluorescence imaging approach. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 2012;46(2):1178–84.
MLA
Evens, Roel, Karel De Schamphelaere, Björn De Samber, et al. “Waterborne Versus Dietary Zinc Accumulation and Toxicity in Daphnia Magna: a Synchrotron Radiation Based X-ray Fluorescence Imaging Approach.” ENVIRONMENTAL SCIENCE & TECHNOLOGY 46.2 (2012): 1178–1184. Print.