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Vaccination against Strongyloides venezuelensis with homologue antigens using new immunomodulators

(2010) JOURNAL OF PARASITOLOGY. 93(3). p.643-647
Author
Organization
Abstract
Control of Strongyloides stercoralis infection is based on antihelminthic treatment. However, the development of an efficient vaccine is an important goal for more effectively managing this disease. Different Strongyloides spp. antigen preparations have been previously tested but without satisfactory results. In the present study, we evaluated whether the doxycholic acid extract of Strongyloides venezuelensis stage 3 larvae was able to protect CD1 mice against a homologue infection. Moreover, we included saponins from Quillaja saponaria (Qs) and immunomodulatory substances, i.e., Phlebodium pseudoaureum hydroalcoholic extract (PAL) or the amino alcohol AA0029, which has an aliphatic chain of 14 carbon atoms with a hydrophilic amino alcohol head, where the amino group is protected with a butoxycarbonyl group. The DOCSv antigen, together with the adjuvant components, was emulsified in a non-mineral water/oil emulsion. We performed duplicate experiments with each of the 2 immunomodulators. The DOCSv antigen used with Qs+PAL induced high levels of protection in terms of fecal egg count reduction (93.2-99.4%), 86-88% in adult worm recovery, and reduction in swelling of the small intestine. Mice vaccinated with the DOCSv antigen, together with Qs+AA0029, showed a 73.7-97.0% reduction in fecal egg count, with reduction in inflammation of the small intestine. High levels of IgG and IgG2a were observed in mice vaccinated with Qs+AA0029+DOCSv, indicating a Th1 immune response. Also, vaccinated mice recognized bands of 34, 39, 43, 95, and 170 kDa in a Western blot.
Keywords
PROTECTIVE IMMUNITY, BALB/C MICE, POLYPODIUM-LEUCOTOMOS EXTRACT, STERCORALIS, MODULATION, RESPONSES

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Citation

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MLA
Vlaminck, Johnny, Julio López-Abán, Ana Lucía Ruano, et al. “Vaccination Against Strongyloides Venezuelensis with Homologue Antigens Using New Immunomodulators.” JOURNAL OF PARASITOLOGY 93.3 (2010): 643–647. Print.
APA
Vlaminck, J., López-Abán, J., Ruano, A. L., del Olmo, E., & Muro, A. (2010). Vaccination against Strongyloides venezuelensis with homologue antigens using new immunomodulators. JOURNAL OF PARASITOLOGY, 93(3), 643–647.
Chicago author-date
Vlaminck, Johnny, Julio López-Abán, Ana Lucía Ruano, Ester del Olmo, and Antonio Muro. 2010. “Vaccination Against Strongyloides Venezuelensis with Homologue Antigens Using New Immunomodulators.” Journal of Parasitology 93 (3): 643–647.
Chicago author-date (all authors)
Vlaminck, Johnny, Julio López-Abán, Ana Lucía Ruano, Ester del Olmo, and Antonio Muro. 2010. “Vaccination Against Strongyloides Venezuelensis with Homologue Antigens Using New Immunomodulators.” Journal of Parasitology 93 (3): 643–647.
Vancouver
1.
Vlaminck J, López-Abán J, Ruano AL, del Olmo E, Muro A. Vaccination against Strongyloides venezuelensis with homologue antigens using new immunomodulators. JOURNAL OF PARASITOLOGY. 2010;93(3):643–7.
IEEE
[1]
J. Vlaminck, J. López-Abán, A. L. Ruano, E. del Olmo, and A. Muro, “Vaccination against Strongyloides venezuelensis with homologue antigens using new immunomodulators,” JOURNAL OF PARASITOLOGY, vol. 93, no. 3, pp. 643–647, 2010.
@article{1988164,
  abstract     = {Control of Strongyloides stercoralis infection is based on antihelminthic treatment. However, the development of an efficient vaccine is an important goal for more effectively managing this disease. Different Strongyloides spp. antigen preparations have been previously tested but without satisfactory results. In the present study, we evaluated whether the doxycholic acid extract of Strongyloides venezuelensis stage 3 larvae was able to protect CD1 mice against a homologue infection. Moreover, we included saponins from Quillaja saponaria (Qs) and immunomodulatory substances, i.e., Phlebodium pseudoaureum hydroalcoholic extract (PAL) or the amino alcohol AA0029, which has an aliphatic chain of 14 carbon atoms with a hydrophilic amino alcohol head, where the amino group is protected with a butoxycarbonyl group. The DOCSv antigen, together with the adjuvant components, was emulsified in a non-mineral water/oil emulsion. We performed duplicate experiments with each of the 2 immunomodulators. The DOCSv antigen used with Qs+PAL induced high levels of protection in terms of fecal egg count reduction (93.2-99.4%), 86-88% in adult worm recovery, and reduction in swelling of the small intestine. Mice vaccinated with the DOCSv antigen, together with Qs+AA0029, showed a 73.7-97.0% reduction in fecal egg count, with reduction in inflammation of the small intestine. High levels of IgG and IgG2a were observed in mice vaccinated with Qs+AA0029+DOCSv, indicating a Th1 immune response. Also, vaccinated mice recognized bands of 34, 39, 43, 95, and 170 kDa in a Western blot.},
  author       = {Vlaminck, Johnny and López-Abán, Julio and Ruano, Ana Lucía and del Olmo, Ester  and Muro, Antonio },
  issn         = {0022-3395},
  journal      = {JOURNAL OF PARASITOLOGY},
  keywords     = {PROTECTIVE IMMUNITY,BALB/C MICE,POLYPODIUM-LEUCOTOMOS EXTRACT,STERCORALIS,MODULATION,RESPONSES},
  language     = {eng},
  number       = {3},
  pages        = {643--647},
  title        = {Vaccination against Strongyloides venezuelensis with homologue antigens using new immunomodulators},
  url          = {http://dx.doi.org/10.1645/GE-2276.1},
  volume       = {93},
  year         = {2010},
}

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